GC cell lines (AGS, NCI-N87, KatoIII) were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). AGS-EBV, an EBV-infected GC cell line,30 (link) was a gift from Dr Shannon C Kenney (Department of Oncology and Medicine, McArdle Laboratory for Cancer Research at the University of Wisconsin, Madison, WI, USA). MKN28, MKN45, SNU16, SNU620, SNU638 and SNU719 cell lines were obtained from the Korean Cell Line Bank (Seoul, Korea). YCCEL1 was a gift from Sun Young Rha at Yonsei Cancer Center, Yonsei University College of Medicine, Seoul, Korea. BGC823, MGC803 and the immortalized normal human gastric epithelial cell line GES-1 were gifts from Oncology Hospital, Beijing University, Beijing, China. Cells were cultured in RPMI 1640, Dulbecco's modified Eagle's medium or McCoy medium (Gibco BRL, Rockville, MD, USA) supplemented with 10% fetal bovine serum (Gibco BRL).
Mkn45
Manufactured by Korean Cell Line Bank
Sourced in United States
The MKN45 is a laboratory equipment product designed for cell culture applications. It provides a controlled environment for the growth and maintenance of cells. The core function of the MKN45 is to provide a controlled temperature, humidity, and atmospheric conditions necessary for cell cultivation.
Automatically generated - may contain errors
Lab products found in correlation
Snu 638, by Korean Cell Line Bank (17 mentions)
Mkn28, by Korean Cell Line Bank (15 mentions)
Snu 216, by Korean Cell Line Bank (14 mentions)
Kato 3, by Korean Cell Line Bank (13 mentions)
Snu 668, by Korean Cell Line Bank (13 mentions)
Snu620, by Korean Cell Line Bank (11 mentions)
Snu 719, by Korean Cell Line Bank (11 mentions)
Mkn74, by Korean Cell Line Bank (11 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (11 mentions)
Snu 484, by Korean Cell Line Bank (10 mentions)
Snu 1, by Korean Cell Line Bank (10 mentions)
Nci n87, by Korean Cell Line Bank (10 mentions)
Snu 601, by Korean Cell Line Bank (9 mentions)
Snu 16, by Korean Cell Line Bank (8 mentions)
Snu 5, by Korean Cell Line Bank (7 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (5 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Rpmi 1640, by Thermo Fisher Scientific (4 mentions)
Fetal bovine serum (fbs), by Welgene (3 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions)
41 protocols using mkn45
1
Gastric Cancer Cell Lines Cultivation
2
Acquisition of GC cell lines
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We purchased the GC cell lines MKN-1 (KCLB No. 80101) and MKN-45 (KCLB No. 80103) from the Korean Cell Line Bank (Seoul, Republic of Korea). Further details are provided in Additional file 1 .
3
Gastric Cancer Tissue Collection
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Total 180 gastric tissues including 100 primary carcinomas, 4 adenomas, 6 hamartomas, 6 hyperplastic polyps, and 64 normal gastric tissues were obtained were obtained from 100 gastric cancer patients and 80 noncancer patients by surgical resection in the Kyung Hee University Medical Center (Seoul, Korea). Signed informed consent was obtained from each patient. Tissue specimens were snap-frozen in liquid N2 and stored at −70 °C until used. Tissue slices were subjected to histopathological review and tumor specimens composed of at least 70% carcinoma cells and adjacent tissues found not to contain tumor cells were chosen for molecular analysis. Fourteen human gastric cancer cell lines (SNU5, SNU16, SNU216, SNU484, SNU601, SNU620, SNU638, SNU719, MKN1, MKN28, MKN45, MKN74, AGS, and KATO-III) were obtained from Korea Cell Line Bank (Seoul, Korea) or American Type Culture Collection (Rockville, MD).
4
Gastric Cancer Cell Line Maintenance
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Human gastric carcinoma cell lines SNU-1 (KCLB no.00001.1), SNU-216, (KCLB no.00216), SNU-601 (KCLB no.00601), MKN-45 (KCLB no. 80103), and AGS (KCLB no.21739) were obtained from the Korean Cell Line Bank (KCLB) of Seoul National University (Seoul, South Korea). Mycoplasma testing was performed for all cell lines used, and mycoplasma were eliminated using MC-210 (WakenBtech Co., Ltd). Cells were maintained in DMEM (Thermo Fisher Scientific, Inc.) containing 10% FBS, sodium bicarbonate (Sigma-Aldrich; Merck KGaA), sodium pyruvate (Gibco; Thermo Fisher Scientific, Inc.), and antibiotics (50 U/ml penicillin and 50 µg/ml streptomycin; Gibco; Thermo Fisher Scientific, Inc.) in a 5% CO2 humidified incubator at 37°C. The culture medium was refreshed every 2–3 days. Mitogen-activated protein kinase (MAPK) inhibitor PD98059 and phosphoinositide 3-kinase (PI3K) inhibitor LY294002 were obtained from Sigma-Aldrich; Merck KGaA. To block the EphA2 function of gastric cancer cells, a novel EphA2 receptor inhibitor ALW-II-41-27 (MedChem Express) was used. For all in vitro studies, ALW-II-41-27 was dissolved in 0.01% DMSO and then diluted to a final concentration of 1 µM.
5
Gastric and Lung Cancer Cell Lines
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Five gastric cancer cell lines (SNU5, SNU620, SNU638, Hs746T, and MKN45) were obtained from the Korean Cell Line Bank. Normal immortalized cell lines (HFE145) were obtained from Dr. Ashktorab (Howard University, Washington, DC, USA). Two non-small cell lung cancer (NSCLC) cell lines, EBC-1 and H1993, were purchased from the Japanese Research Resources Bank and the American Type Culture Collection, respectively. All cell lines were authenticated by short tandem repeat analyses in Korea Genome Information Institute and were checked for mycoplasma using the e-Myco VALID Mycoplasma PCR Detection Kit (#25245; iNtRon Biotechnology, Inc., Seongnam-si, Korea).
6
Cultivation of Human Gastric Cancer Cell Lines
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All human gastric cancer cell lines (AGS, SNU16, SNU216, SNU638, MKN45, and MKN74) were purchased from the Korean Cell Line Bank (KCLB; Seoul, Republic of Korea) and cultured in RPMI-1640 (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% penicillin/streptomycin (Gibco). Cells were grown at 37 °C in a 5% CO2 incubator, under humidified conditions.
7
Culturing Human Cancer Cell Lines
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The human gastric cancer cell lines, MKN-45 and SNU-638, and colorectal cancer cell lines, SW620, SNU-C5, and LoVo, were obtained from the Korean Cell Line Bank (Seoul, Korea) and were cultured in a 37 °C incubator with 5% CO2. Cells were grown in RPMI 1640 with 10% heat-inactivated fetal bovine serum, 100 U/mL penicillin and 100 μg/mL streptomycin. Culture medium was replaced approximately every 48 h.
8
Cell Line Cultivation for Cancer Research
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9
Culturing Cancer Cell Lines
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Six cancer cell lines, human breast adenocarcinoma (MDA-MB-231), human follicular thyroid carcinoma (FTC133), human fibrosarcoma (HT1080), human gastric adenocarcinoma (MKN45), human prostate adenocarcinoma (PC3), and human lung adenocarcinoma (A549), were obtained from the Korean Cell Line Bank (Seoul, Korea). All cells were grown in Dulbecco’s modified Eagle medium (high glucose; WelGENE Inc., Daegu, Korea) or RPMI-1640 medium (WelGENE Inc.) supplemented with 10% FBS (WelGENE Inc.) and 1% penicillin/streptomycin at 37 °C and 5% fully humidified CO2.
10
Characterization of Human Gastric Cancer Cell Lines
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Human gastric cancer cell lines (SNU-1, -5, -16, 216, -484, -601, -620, -638, -668, -719, AGS, MKN45, KATO-III, and N87) were purchased from the Korean Cell Line Bank (Seoul, Korea). The identities of the cell lines were authenticated by DNA fingerprinting analysis [19 (link)]. All cell lines were banked and passaged for less than 6 months before use. The cells were cultured in RPMI 1640 medium (Thermo Fisher Scientific Inc., Waltham, MA) supplemented with 10% fetal bovine serum (Welgene, Daegu, Korea) and 10 μg/mL gentamicin (Cellgro, Manassas, VA) at 37°C and 5% CO2.
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