Quantum fx ct

Manufactured by PerkinElmer

Sourced in United States

The Quantum FX μCT is a micro-computed tomography (μCT) system designed for high-resolution, non-destructive imaging of small samples. It utilizes advanced X-ray technology to capture detailed three-dimensional (3D) images of the internal structure of a wide range of materials and specimens.

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Lab products found in correlation

Analyzepro software, by AnalyzeDirect (2 mentions)

3 protocols using quantum fx ct

Quantifying Visceral and Subcutaneous Adiposity

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Both in prevention and reversion studies, micro-computed tomography (micro-CT) scans were performed to assess the volumes of the visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) depots.
Four mice from each group were randomly selected and anesthetized with 5% isoflurane. Transverse micro-CT images of the abdomen from L1 to L5 were obtained by the micro-CT scanner Quantum FX µCT (Perkin-Elmer, Hopkinton, MA, USA). Voltage was set at 50 kV and current was set at 200 μA and the images were captured over a 4.5 min interval. Analysis of micro-CT images was conducted with AnalyzePro software (AnalyzeDirect, Overland Park, KS, USA). Visceral and subcutaneous adipose tissue were segmented in the sagittal plane and tissue volumes were expressed relative to body mass [31 (link)]. Experimental data from micro-CT were provided by ATeN Center—Università di Palermo.

Terzo S., Caldara G.F., Ferrantelli V., Puleio R., Cassata G., Mulè F, & Amato A. (2018). Pistachio Consumption Prevents and Improves Lipid Dysmetabolism by Reducing the Lipid Metabolizing Gene Expression in Diet-Induced Obese Mice. Nutrients, 10(12), 1857.

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Low-dose X-ray Lung Function Imaging

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Low-dose x-ray-based in vivo lung function measurement (XLF) was performed as previously described by Dullin et al. 30 (link). Briefly, mice were anaesthetised with isoflurane and the anaesthesia was adjusted to keep the breathing rate to approximately 1.4 s per breathing cycle. Cinematic x-ray images (1024 images) were acquired continuously with a rate of 30 images per second with a QuantumFX µCT (Perkin Elmer) using the following parameters: FOV 20×20 mm², x-ray tube voltage 90 kVp and tube current 40 μA, resulting in an entrance dose of about 6 mGy. Data was analysed using the software XLF 30 (link). The dynamic parameter t_in[%] represents the relative inhalation time, meaning the ratio of inhalation time to total length of a breathing event.
For diaphragm motion analysis, the average and the standard deviation (SD) of the changes in brightness for each pixel over time was calculated from the acquired x-ray movies using FIJI (National Institutes of Health, Bethesda, USA) 31 (link). Thereby, moving areas at the interface of dark and bright regions between the lungs and soft tissues beneath the lung, which we defined as the diaphragm, show very high SDs compared to static areas 32 (link). The average height of the area with high SD was measured at six independent positions (three per side) and was used as a parameter representing the average diaphragm movement (s_dia).

Napp J., Markus M.A., Heck J.G., Dullin C., Möbius W., Gorpas D., Feldmann C, & Alves F. (2018). Therapeutic Fluorescent Hybrid Nanoparticles for Traceable Delivery of Glucocorticoids to Inflammatory Sites. Theranostics, 8(22), 6367-6383.

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Micro-CT Assessment of Adipose Tissue Volumes

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Micro-computed tomography (micro-CT) scans were performed to assess the volumes of the visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT). A representative sample of rats from each group were randomly selected at T1 and anesthetized with 5% isoflurane. Transverse micro-CT images of the abdomen from L1 to L5 were obtained by the micro-CT scanner Quantum FX µCT (Perkin-Elmer, Hopkinton, MA, USA) in the animal facility of ATeN Center—University of Palermo. Voltage was set at 50 kV and current was set at 200 μA and the images were captured over a 4.5 min interval. Analysis of micro-CT images was conducted with AnalyzePro software (AnalyzeDirect, Overland Park, KS, USA). Visceral and subcutaneous adipose tissue was segmented in the sagittal plane and measurements of volume obtained with the Region of Interest mode. Tissue volumes are expressed relative to body mass. Procedures were performed following standardised protocols [45 (link)].

Di Majo D., Sardo P., Giglia G., Di Liberto V., Zummo F.P., Zizzo M.G., Caldara G.F., Rappa F., Intili G., van Dijk R.M., Gallo D., Ferraro G, & Gambino G. (2022). Correlation of Metabolic Syndrome with Redox Homeostasis Biomarkers: Evidence from High-Fat Diet Model in Wistar Rats. Antioxidants, 12(1), 89.

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