HIV RNA levels were measured in CVL supernatants using the Abbott m2000 HIV-1 RealTime System (Abbott Molecular, Des Plaines, IL) with LLOD of 40 copies/ml. CMV DNA was detected in CVL cell pellets using the artus CMV TM PCR kit (Qiagen, Inc, Valencia, CA) on the Qiagen Rotor Gene instrument with a LLOD of 300 IU/mL. The amplification target was a 105 base pair region of the immediate-early exon 4 gene of the CMV genome. HSV was detected in CVL cell pellets using a homogenous kinetic PCR designed to amplify a conserved sequence within the polymerase gene (pol) of HSV-1 and HSV-2 (Emory Center For AIDS Research).
M2000 hiv 1 realtime system
Manufactured by Abbott
The Abbott m2000 HIV-1 RealTime System is a laboratory instrument designed for the detection and quantification of HIV-1 RNA in human plasma and serum samples. The system utilizes real-time PCR technology to perform automated nucleic acid extraction, amplification, and detection.
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Lab products found in correlation
2 protocols using m2000 hiv 1 realtime system
1
Quantification of HIV, CMV, and HSV in CVL
2
Quantifying HIV-1 RNA in Cervical Fluid
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