Gemcitabine hydrochloride was purchased from AK Scientific (Union City, CA). Dodecanol (DC), triethyl-amine (TEA), 1-ethyl-3-(3- (dimethylamino)propyl) carbodiimide (EDC), hydroxybenzotriazole (HOBT), 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), benzyl bromide, 2,2-bis(hydroxymethyl) propionic acid, and methoxy poly(ethylene glycol) (mPEG, Mn = 5000, PDI = 1.03) were purchased from Sigma-Aldrich (St. Louis, MO) and used without further purification. Matrigel matrix basement membrane was procured from Corning (Chicago, IL). All other chemicals were of analytical grade and purchased from Sigma-Aldrich (St. Louis, MO).
Methoxy poly ethylene glycol mpeg
Manufactured by Merck Group
Sourced in United States
Methoxy poly(ethylene glycol) (mPEG) is a synthetic polymer that consists of a methoxy group attached to a polyethylene glycol (PEG) chain. It is a colorless, viscous liquid with a molecular weight range typically between 2,000 and 40,000 g/mol. mPEG is used as a versatile reagent in various applications, including drug delivery, protein modification, and surface modification of biomaterials.
Automatically generated - may contain errors
Lab products found in correlation
2 2 bis hydroxymethyl propionic acid, by Merck Group (2 mentions)
Benzyl bromide, by Merck Group (1 mentions)
1 8 diazabicyclo 5.4.0 undec 7 ene dbu, by Merck Group (1 mentions)
Matrigel basement membrane matrix, by Corning (1 mentions)
ε caprolactone cl, by Merck Group (1 mentions)
1 1 1 3 3 3 hexafluoro 2 propanol hfip, by Merck Group (1 mentions)
Sn oct 2, by Merck Group (1 mentions)
Dexamethasone 21 phosphate disodium salt dex, by Merck Group (1 mentions)
Ethyl acetate, by Merck Group (1 mentions)
Sodium azide, by Merck Group (1 mentions)
Sodium hydride, by Merck Group (1 mentions)
Copper 2 acetate monohydrate, by Merck Group (1 mentions)
Propargyl bromide, by Merck Group (1 mentions)
Doxorubicin hydrochloride, by Merck Group (1 mentions)
Avance 3 400 spectrometer, by Bruker (1 mentions)
N n diisopropylcarbodiimide dic, by GL Biochem (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Tetrahydrofuran, by Thermo Fisher Scientific (1 mentions)
Pepsin, by Merck Group (1 mentions)
Methyl acrylate, by Merck Group (1 mentions)
14 protocols using methoxy poly ethylene glycol mpeg
1
Gemcitabine Conjugated Polymer Synthesis
2
Synthesis and Characterization of MPEG-PLA-CL Scaffolds
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The initiator methoxy poly(ethyleneglycol) (MPEG, average molecular weight Mn = 750 g/mol) and the catalyst Sn(Oct)2 were purchased from Sigma Aldrich (St. Louis, MO, USA) and used without further purification. Lactide (LA; Boehringer Ingelheim, Blanquefort, France) was recrystallized twice in ethyl acetate, and ε-caprolactone (CL; Aldrich, Milwaukee, WI, USA) was distilled over CaH2 under reduced pressure. 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP, >99%) was obtained from Fluka (Sigma-Aldrich, MO, USA) as an electrospinning solvent. Collagenase from Clostridium histolyticum (C7657, >1200 CDU/mg) was purchased from Sigma Aldrich. Dexamethasone 21-phosphate disodium salt (Dex) and silver-sulfadiazine (AgS) were purchased from Sigma-Aldrich (St Louis, MO, USA). Other materials were used without further purification.
3
Gemcitabine and Vismodegib Conjugation Protocol
4
Azido-Functionalized Coumarin-PEG Conjugation
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The following materials
were used to carry out the different syntheses: 4-bromomethyl-7-methoxycoumarin
(97%, Sigma Aldrich), sodium azide (>99.5%, Sigma Aldrich), methoxy
polyethylene glycol (mPEG, Mn = 2000 g/mol, Sigma
Aldrich), propargyl bromide (80% in toluene, Sigma Aldrich), sodium
hydride (NaH, 60% in mineral oil, Sigma Aldrich), ethyl acetate (anhydrous,
99.8%, Sigma Aldrich), and carbon dioxide (industrial grade ≥99%,
Carburos Metálicos). The catalyst used to carry out the cycloaddition
reaction was copper(II) acetate monohydrate (Sigma Aldrich).
were used to carry out the different syntheses: 4-bromomethyl-7-methoxycoumarin
(97%, Sigma Aldrich), sodium azide (>99.5%, Sigma Aldrich), methoxy
polyethylene glycol (mPEG, Mn = 2000 g/mol, Sigma
Aldrich), propargyl bromide (80% in toluene, Sigma Aldrich), sodium
hydride (NaH, 60% in mineral oil, Sigma Aldrich), ethyl acetate (anhydrous,
99.8%, Sigma Aldrich), and carbon dioxide (industrial grade ≥99%,
Carburos Metálicos). The catalyst used to carry out the cycloaddition
reaction was copper(II) acetate monohydrate (Sigma Aldrich).
5
Synthesis and Purification of Doxorubicin-Loaded Polymers
6
Camptothecin-Loaded Amphiphilic Nanomedicine
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Methoxy poly(ethylene glycol) (mPEG, MW = 350, 500, 2000) was purchased from Sigma-Aldrich without further purification. Camptothecin (CPT) was purchased from Sichuan Jiangyuan Natural Products Co. (Sichuan, China). α-Cyclodextrin (α-CD) was purchased from Aladdin Reagent Co., Ltd. (Shanghai, China). N,N'-Diisopropylcarbodiimide (DIC) was purchased from GL Biochem. Ltd. (Shanghai, China). All other reagents and solvents used in the study were analytical grade and obtained from commercial sources. 1H and 13C NMR were measured on a Bruker AVANCE III-400 spectrometers (Bruker Co., Karlsruhe, Germany).
7
Synthesis and Characterization of mPEG-Pepsin Conjugate
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Methoxy polyethylene glycol (mPEG, molecular weight = 5000 Da) and 4-nitrophenyl chloroformate (NPC) were purchased from Sigma Aldrich (St. Louis, MO, USA). Ethylene diamine (EDA), methyl acrylate (MA) and pepsin (PS) were obtained from Merck (Darmstadt, Germany). Methanol and tetrahydrofuran (THF) were purchased from Fisher Scientific (NJ, USA). Dialysis membrane with molecular weight cut-off (MWCO) of 3.5 kDa was bought from Spectra/Por® (Texas City, TX, USA). Lamivudine 99.5% (3TC, LOT:R088C0) and zidovudine 99.6% (AZT, LOT:R052L0) were U.S.P grade.
For cell culture, Dulbecco’s modified Eagle’s medium (DMEM), penicillin-streptomycin solution (10,000 U/mL), fetal bovine serum (FBS) were purchased from Sigma-Aldrich (Singapore). Other chemicals and solvents were used without further purification.
For cell culture, Dulbecco’s modified Eagle’s medium (DMEM), penicillin-streptomycin solution (10,000 U/mL), fetal bovine serum (FBS) were purchased from Sigma-Aldrich (Singapore). Other chemicals and solvents were used without further purification.
8
Preparation of Docetaxel-Loaded PLA-PEG Nanoparticles
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D,L -lactide (3,6-dimethyl-1,4-dioxane-2,5-dione, 99%,) was purchased from Corbion (Gorinchem, The Netherlands) and recrystallized in butyl acetate (Component Reactive, Moscow, Russia) before use. Methoxy poly(ethylene glycol) (mPEG) with molar mass of 5 kDa was purchased from Sigma-Aldrich (St. Louis, MO, USA) and dried at 150 °C under vacuum for 1 h before use. Stannous (II) 2-ethylhaxanoate (SnOct2) (Sigma-Aldrich, St. Louis, MO, USA) and poly(vinyl alcohol) (PVA) (87–90% hydrolyzed) with molar mass of 30–70 kDa (Sigma-Aldrich, St. Louis, MO, USA) were used as received. Docetaxel trihydrate (DTX) (99%) was purchased from Qilu Pharmaceutical Co., Ltd. (Jinan, Shandong, China) and used as received. Cryoprotectants D(-)-mannitol (Honeywell Burdick & Jackson, Seelze, Germany), mPEG with molar mass of 2 kDa (Sigma-Aldrich, St. Louis, MO, USA), and PEG with molar mass of 10 kDa (Sigma-Aldrich, St. Louis, MO, USA) were used as received. All organic solvents (Component Reactive, Moscow, Russia) were of analytical grade and used without further purification. Double distilled water was used for all experiments.
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9
Synthesis and Characterization of MPEG
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Methoxy polyethylene glycol (MPEG, analytical grade, Mn = 350, 550, 750, 2000, and 5000 g/mol), triethylamine (TEA), methanol (MeOH), n-hexane, tetrahydrofuran (THF), chloroform (CHCl3), deuterated chloroform (CDCl3), terephthaloyl chloride (TPC; 203.02 g/mol), and isophthaloyl chloride (IPC, 203.02 g/mol) were obtained from Sigma-Aldrich (Milwaukee, WI, US).
10
Synthesis and Characterization of Silver Nanoparticles
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Methoxypolyethylene glycol (mPEG) with molecular weight 2000 or 5000 and 2,4,6-trichloro-s-triazine (cyanuric chloride) were purchased from Sigma-Aldrich. The AgNO3 salt was purchased from Merck. All of the chemicals used were analytical grade and were used without further purification. A Fourier transform infrared (FT-IR) spectrometer (Nicolet, NEXUS-670); a UV-vis spectrophotometer (Shimadzu UV-1208 model); and an X-ray diffractometer (XRD) (X’Pert PRO, PANalytical BV, Almelo, the Netherlands) using CuKα radiation were used for the characterization of AgNPs.39 (link) Transmission electron microscopy (TEM), equipped with energy dispersive X-ray (EDX) analysis, was used to study the size, morphology, and composition of the AgNPs. Histograms of the size distribution of the AgNPs were calculated from the TEM images by measuring the diameters of at least 50 particles.39 (link)
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