Rtx 5

Chemicals including NEt₃, NBu₄Br, NBu₄Cl, NBu₄I, NBu₄PF₆, epoxides and CO₂ are commercially available and used directly without further purification. Gas chromatography (GC) was performed on a Shimadzu GC-2014 equipped with a capillary column (RTX-5, 30 m × 0.25 μm) using a flame ionization detector.

The compounds separated by TLC were identified using gas chromatograph. The purified methanolic crude extract was subjected to GC-MS analysis to identify the bioactive compounds. The GC–MS-MS analysis of the crude extracts was carried out in a Shimadzu GC-MS-QP 2010 Plus fitted with a RTX-5 (60 m × 0.25 mm × 0.25 µm) capillary column in JNU, New Delhi. The instrument was set to an initial temperature of 70 °C, and maintained at this temperature for 2 min. At the end of this period the oven temperature was rose up to 280 °C, at the rate of an increase of 5 °C/min, and maintained for 9 min. Injection port temperature was ensured as 260 °C and Helium flow rate as 1 mL/min. The ionization voltage was 70 eV. The samples were injected in split mode as 10:1. Mass spectral scan range was set at 45–450 (m/z). The identification of bioactive compounds present in the extracts was performed by comparing the mass spectra with data from NIST05 (National Institute of Standards and Technology, US), WILEY 8, and FFNSC1.3 (Flavour and Fragrance Natural and Synthetic Compounds) libraries. The name, molecular weight and structure of the components of the test material were ascertained.