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100cxii

Manufactured by JEOL
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Sourced in Japan, United Kingdom, United States

The JEOL-100CXII is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of materials at the nanoscale. It features a 100 kV electron beam and advanced optical systems for optimized performance. The JEOL-100CXII provides users with the capability to visualize and characterize a wide range of samples with precision and accuracy.

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Lab products found in correlation

Lx112 resin, by Ladd Research Industries (5 mentions) Ultracut e microtome, by Reichert Technologies (3 mentions) Ultramicrotome, by Leica (2 mentions) Reichert ultracut e, by Leica (2 mentions) Nano zs90, by Malvern Panalytical (2 mentions) Megaview 3 digital camera, by Olympus (1 mentions) Formvar coated 200 mesh copper grid, by Electron Microscopy Sciences (1 mentions) Cryoplunge 3, by Ametek (1 mentions) Jem 2100 electron microscope, by JEOL (1 mentions) Ultrascan 4000 ccd camera, by Ametek (1 mentions) Eclipse e200 microscope, by Nikon (1 mentions) C4742 95, by Hamamatsu Photonics (1 mentions) Coolpix 8400, by Nikon (1 mentions) Durcupan, by Merck Group (1 mentions) Reichert ultracut s ultramicrotome, by Leica (1 mentions) Nanodrop spectrometer, by Thermo Fisher Scientific (1 mentions) Em uc7, by Leica (1 mentions) Lc 2010aht, by Shimadzu (1 mentions) Diamond knife, by Electron Microscopy Sciences (1 mentions) Reichert supernova, by Leica (1 mentions)

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JEOL 100CXII microscope (4 citations)

86 protocols using 100cxii

1

Scanning Electron Microscopy of Pollen Grains

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The morphological features of the pollen grains were observed using SEM (Jeol 100 CXII). The method for sampling was the same as that performed in the experiment on pollen vitality. The pollen grains from the same cultivar were mixed. For each cultivar, 3 visual fields were observed (× 2000), and in each field, 5 pollen grains were measured (the pollen grains were well arranged for ease of measurement). The pollen grains were platinized with an ion sputtering equipment (ETD-2000) for SEM, and no less than 20 pollen grains for each cultivar were examined. The shriveled pollen grains with great deformities under the scanning electron microscope were defined deformed. Under the microscope (× 200), five visual fields were randomly selected, and no less than 100 pollen grains in each field were observed.
Deng Q., Li J., Gao C., Cheng J., Deng X., Jiang D., Li L, & Yan P. (2020). New perspective for evaluating the main Camellia oleifera cultivars in China. Scientific Reports, 10, 20676.
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2

Ultrastructural Analysis of Earthworm Tissues

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Tissue localization was evaluated on semithin components, and ultrathin parts were created as needed. Leica AG ultramicrotome was used to cut ultrathin pieces (50–80 nm) that were stained with uranyl acetate and lead citrate. TEM (JEOL, 100 CXII) at 80 kV was used to earthworm parts from different groups. Electron micrographs were acquired, reconstructed, and evaluated to study the selected semithin regions using Photoshop software.
Salem S.H., El-Maraghy S.S., Abdel-Mallek A.Y., Abdel-Rahman M.A., Hassanein E.H., Al-Bedak O.A, & El-Aziz F.E. (2022). The antimicrobial, antibiofilm, and wound healing properties of ethyl acetate crude extract of an endophytic fungus Paecilomyces sp. (AUMC 15510) in earthworm model. Scientific Reports, 12, 19239.
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3

Intracellular TEM Analysis of EGFP-H1299 Cells

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Intracellular transmission electron microscopy (TEM): EGFP-expressing H1299 cells were seeded in six-well plates at a density of 400,000 cells/well and cultured in antibiotic-free media. Cells were treated 24 hours post-seeding with 200 μL transfection solutions (Opti-MEM, 200 nM siRNA and 200 μg/mL SNP) and incubated at 37°C, 5% CO2 and 100% relative humidity. Cells were trypsinized 24 hours post-transfection and pelleted by centrifugation (200 RCF) at 4°C. Samples were fixed using 2.5% formaldehyde/glutaraldehyde in DPBS, stained with 1% osmium tetroxide in DPBS, dehydrated through a graded series of ethanol concentrations and embedded in Spurr resin. Samples were sectioned to a thickness of ~90 nm using an RMC MYX ultramicrotome and placed onto a 200-mesh formvar coated copper grid. Samples were additionally stained with uranyl acetate and lead citrate. Images were acquired using a JEOL 100CXII transmission electron microscope operating at an accelerating voltage of 100 keV and equipped with an Olympus MegaView III digital camera. Energy dispersive X-ray spectroscopy (EDS) analysis was performed on JEOL 2200FS transmission electron microscope operating at an accelerating voltage of 200 keV.
Vocelle D., Chesniak O.M., Malefyt A.P., Comiskey G., Adu-Berchie K., Smith MR I.I.I., Chan C, & Walton S.P. (2016). Dextran functionalization enhances nanoparticle-mediated siRNA delivery and silencing. Technology, 4(1), 42.
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4

Characterizing SNP Nanoparticles by TEM

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SNPs were dispersed in acetic acid (pH 4.75) at a concentration of 0.5 mg/ml and incubated at RT. After 16 hours, the samples were centrifuged and washed three times with DPBS. Samples were prepared for imaging by placing 5 μL of the sample onto a 200-mesh formvar coated copper grid and air dried overnight. Images were acquired using a JEOL 100CXII transmission electron microscope operating at an accelerating voltage of 100 keV and equipped with an Olympus MegaView III digital camera.
Vocelle D., Chesniak O.M., Malefyt A.P., Comiskey G., Adu-Berchie K., Smith MR I.I.I., Chan C, & Walton S.P. (2016). Dextran functionalization enhances nanoparticle-mediated siRNA delivery and silencing. Technology, 4(1), 42.
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5

Ultrastructure Visualization of Organelles

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Isolated organelles were fixed in 2.5% glutaraldehyde in 100 mM sodium cacodylate, pH 7.43 maintained isomolar by addition of 0.25 M sucrose. Samples were post-fixed in 1% osmium tetroxide in 100 mM sodium cacodylate, pH 7.43, followed by 1% uranyl acetate. After ethanol dehydration and embedment in LX112 resin (LADD Research Industries, 21210), ultrathin sections were cut on a Reichert Ultracut E and were stained with uranyl acetate followed by lead citrate. All grids were viewed on a JEOL 100CX II transmission electron microscope at 80 kV.
Caballero B., Bourdenx M., Luengo E., Diaz A., Sohn P.D., Chen X., Wang C., Juste Y.R., Wegmann S., Patel B., Young Z.T., Kuo S.Y., Rodriguez-Navarro J.A., Shao H., Lopez M.G., Karch C.M., Goate A.M., Gestwicki J.E., Hyman B.T., Gan L, & Cuervo A.M. (2021). Acetylated tau inhibits chaperone-mediated autophagy and promotes tau pathology propagation in mice. Nature Communications, 12, 2238.
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6

Visualization of T6SS in Francisella

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Fractions of T6SS purified as described above from cultures of wild-type F. novicida or F. novicida expressing IglA-GFP were diluted 100-fold with TBS and applied to glow discharged formvar coated nickel grids. After 5 min, the grids were washed with TBS, blocked for 30 min with 20 mM HEPES, 0.15 M NaCl containing 1% BSA (HBS-BSA), and stained with rabbit anti-GFP (1:1000) for 60 min in the HBS-BSA. The grids were washed with HBS; stained for 60 min with 5 nm protein A gold in HBS-BSA (University of Utrecht); washed with HBS; negatively stained with 2% uranyl acetate; and examined by TEM using a JEOL 100 CXII.
Clemens D.L., Ge P., Lee B.Y., Horwitz M.A, & Zhou Z.H. (2015). Atomic Structure and Mutagenesis of T6SS Reveals Interlaced Array Essential to Function. Cell, 160(5), 940-951.
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7

TEM Imaging of Transethosomal Vesicles

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TEM (100 CX II; JEOL, Tokyo, Japan) was used for imaging of the TRE vesicles. Transethosomal dispersion (selected formulation) was diluted tenfold utilizing distilled water, then a drop was placed on a copper grid with a 300-mesh carbon coating and allowed to sit for a minute to allow some of the vesicles to stick to the carbon substrate. A piece of filter paper was used to remove extra dispersion, and the grid was then rinsed twice in deionized water for 3–5 seconds. The sample was then examined under a microscope utilizing 10–100× magnification and an accelerating voltage of 100 kV.
Hassan A.S., Hofni A., Abourehab M.A, & Abdel-Rahman I.A. (2023). Ginger Extract–Loaded Transethosomes for Effective Transdermal Permeation and Anti-Inflammation in Rat Model. International Journal of Nanomedicine, 18, 1259-1280.
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8

Exosome Visualization via Negative Staining

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Negative staining technique was employed to visualize the Exos. An enriched exosomal suspension in filtered DPBS (Dulbecco's phosphate-buffered saline) was dispensed on carbon-coated electron microscopy grids on parafilm and left to absorb for 10 min at room temperature then transferred to a drop of Uranyless® solution for 1 min and left to air dry. Excess stain was blotted away. Imaging was performed using a JEOL 100CX II transmission electron microscope (TEM) at 100 kV.
Biagioni A., Laurenzana A., Menicacci B., Peppicelli S., Andreucci E., Bianchini F., Guasti D., Paoli P., Serratì S., Mocali A., Calorini L., Del Rosso M., Fibbi G., Chillà A, & Margheri F. (2020). uPAR-expressing melanoma exosomes promote angiogenesis by VE-Cadherin, EGFR and uPAR overexpression and rise of ERK1,2 signaling in endothelial cells. Cellular and Molecular Life Sciences, 78(6), 3057-3072.
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9

Electron Microscopy Sample Preparation

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Tissues (1 mm3) were fixed in 2.5% glutaraldehyde in 100 mmol/L sodium cacodylate, pH7.4, and postfixed in 1% osmium tetroxide followed by 1% uranyl acetate. After ethanol dehydration, the tissues were embedded in LX112 resin (LADD Research Industries). Ultrathin sections were stained with uranyl acetate followed by lead citrate. All grids were viewed on a JEOL 100CX II transmission electron microscope at 80 kV.
Chen K., Yuan R., Zhang Y., Geng S, & Li L. (2017). Tollip Deficiency Alters Atherosclerosis and Steatosis by Disrupting Lipophagy. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 6(4), e004078.
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10

KSHV Infection Kinetics in HMVEC-d Cells

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HMVEC-d cells grown in 6 cm petri-dishes were washed three to four times with medium without serum. The cells were infected with purified KSHV (20 DNA copies/cell) at 37°C for 5 and 10 min in serum free medium. After each time point, cells were washed, fixed immediately with 2% paraformaldehyde and 2.5% gluteraldehyde in 0.1M Cacodylate buffer at RT by adding gently from the edge of the dish and kept for 20 to 30 minutes at RT. These were postfixed in 1% osmium tetroxide, dehydrated in a graded ethanol series, and embedded in 812 resin. Thin sections were made and visualized under a JEOL 100CXII transmission electron microscope.
Bandyopadhyay C., Valiya-Veettil M., Dutta D., Chakraborty S, & Chandran B. (2014). CIB1 Synergizes with EphrinA2 to Regulate Kaposi's Sarcoma-Associated Herpesvirus Macropinocytic Entry in Human Microvascular Dermal Endothelial Cells. PLoS Pathogens, 10(2), e1003941.
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