Monosodium glutamate (G1626, Sigma) was dissolved in sterile isotonic saline (0.9% saline). 17β-estradiol (E-2758, Sigma) was dissolved in ethanol and diluted to 10% in sterile saline immediately before administration. Genistein (253493, J&K Scientific, Beijing, China) was dissolved in 5% dimethyl sulfoxide and 95% polyethylene glycol 400.
Genistein
Manufactured by J&K Scientific
Sourced in China
Genistein is a natural isoflavone compound commonly found in soybeans and other legumes. It functions as a tyrosine kinase inhibitor, which can affect various cellular processes. The core function of genistein is to serve as a research tool for studying its potential biological activities.
Automatically generated - may contain errors
Lab products found in correlation
Monosodium glutamate g1626, by Merck Group (1 mentions)
17β estradiol e 2758, by Merck Group (1 mentions)
Rabbit anti cd31 antibody, by Abcam (1 mentions)
Anti cd44 fitc, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 647 conjugated goat anti rabbit igg, by Abcam (1 mentions)
Solutol hs 15, by BASF (1 mentions)
Doxorubicin hydrochloride, by Meilun (1 mentions)
Carboxymethylcellulose sodium salt, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Carbamazepine, by J&K Scientific (1 mentions)
Male sprague dawley rats, by Shanghai Laboratory Animal Center (1 mentions)
3 protocols using genistein
1
Preparation of Bioactive Compounds
2
Comprehensive Biomaterial Characterization for Cell Studies
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CS was purchased from Aladdin biochemical technology Co., Ltd. (Shanghai, China); Doxorubicin hydrochloride was purchased from Meilun Biotechnology (Dalian, China); BSA (Albumin Bovine V) was purchased from BioFroxx (Einhausen, Germany); Soybean oil for injection was purchased from Yuanye Bio-Technology Co., Ltd. (Shanghai, China); Solutol HS15 was purchased from BASF (Ludwigshafen, Germany); 3-(4,5-Dimethyl-2-thiazolyl)−2,5-diphenyl-2H-tetrazolium bromide (MTT), 4,6-diamidino-2-phenylindole (DAPI), monensin sodium and genistein were purchased from J&K Scientific Ltd.; Mouse antibodies including anti-CD44, FITC anti-CD44 and Rhodamine-labeled secondary antibodies were purchased from Affymetrix eBioscience (San Diego, USA). rabbit anti-CD31 antibody and Alexa Fluor 647 conjugated goat anti-rabbit IgG were purchased from Abcam (Cambridge, UK). Other reagents were analytical or HPLC grade and purchased commercially.
3
Celecoxib Pharmacokinetic Evaluation
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Celecoxib was purchased from Perfermker (Shanghai, China); genistein and carbamazepine (IS) were purchased from J&K Chemical (Beijing, China); and carboxy methylcellulose sodium salt (CMC), acetonitrile (ACN), formic acid, and other MS-using chemicals were purchased from Sigma-Aldrich Company (Shanghai, China). The primary standard stock solution of celecoxib (1 mg/ml) and IS (500 ng/ml) used in in vitro experiments were prepared by dissolving in methanol. Celecoxib and genistein used in the in vivo experiment were dissolved by 5% CMC.
The male Sprague-Dawley (SD) rats (240 ± 30 g) were purchased from Shanghai Laboratory Animal Center (SLAC). Before starting experiments, they were raised for three weeks in laboratory to minimize the influence of transportation process.
The male Sprague-Dawley (SD) rats (240 ± 30 g) were purchased from Shanghai Laboratory Animal Center (SLAC). Before starting experiments, they were raised for three weeks in laboratory to minimize the influence of transportation process.
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