Mushrooms (white button and Portobello) were produced at the Centro Tecnológico de Investigación del Champiñón de La Rioja (CTICH), following local industry standard cultivation methods. Clean carpophores were cut into small pieces, freeze-dried, and ground into a dry powder that was sent to Research Diets (New Brunswick, NJ, USA). Pellets for three different diets were generated under the supervision of professional nutritionists: (i) a regular basic diet containing 15% fat (D11112201, Open Standard Diet, Research Diets), (ii) a basic diet supplemented with 5% (w/w) freeze-dried white button mushroom powder, and (iii) a basic diet supplemented with 5% (w/w) freeze-dried Portobello mushroom powder. Nutritional composition of the three diets is shown in Table 1 .
D11112201
Manufactured by Research Diets
Sourced in United States
The D11112201 is a specialized laboratory equipment designed for research purposes. It functions as an automated feed dispenser, capable of precisely measuring and distributing controlled portions of animal feed or other research materials. The device is engineered to ensure accurate and consistent delivery of the required substances, making it a valuable tool for scientific investigations and experimentation.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using d11112201
1
Mushroom Powder Dietary Supplementation
2
Liver Analysis of RAGE-Deficient Obese Mice
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We analysed the livers of mice previously used for another study on adipose tissue. Physiological parameters and methods are reported in Gaens et al.5 (link). Briefly, obese RAGE-deficient mice (RAGE−/− LeptrDb−/−) were generated by crossing obese, non-insulin dependent diabetic C57BLKS-LeptrDb (LeptrDb−/−) (Charles River, Maastricht, the Netherlands) with RAGE−/− mice (obtained from Heidelberg University) for several generations. All mice were fed ad libitum with a standard control diet (catalog #D11112201; Research Diets Inc., New Brunswick, NJ, USA). At 13 weeks of age, lean control C57BLKS (RAGE+/+LeptrDb+/+), obese (RAGE+/+LeptrDb−/−) and their littermate obese RAGE-deficient (RAGE−/− LeptrDb−/−) mice were sacrificed by CO2/O2 inhalation followed by exsanguination via cardiac puncture, plasma was collected and liver was removed and sectioned for following analysis. The animal protocol was approved by the Ethic Committee of Maastricht University. All animal experiments and methods were in compliance with the guidelines from the Directive 2010/63/EU of the European Parliament on the protection of animals used for scientific purposes and follow the ARRIVE guidelines.
3
Determining Dietary Iron Deficiency
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Two diets were purchased from Research Diets (New Brunswick, NJ, USA): Open Standard Diet with 15 kcal% Fat—D11112201, used as iron-balanced diet (Control diet), and the same type of diet with no added iron—D15071503 (Iron deficient diet). Ingredients of the two diets as specified by a manufacturer Research Diets Inc. (New Brunswick, NJ, USA) are presented in Table 1 . Before the experiment, samples of both diets were taken for the purpose of proximate analysis and mineral content. Proximate analysis was carried out in our laboratory by standard procedures [22 ]: dry matter on oven drying at 95–100 °C (AOAC Official method 934.01), crude protein by copper catalyst Kjeldahl method (AOAC Official method 984.13), crude fat to ether extract by extraction in petroleum ether (AOAC Official method 920.39), crude fibre by fritted glass crucible method (AOAC Official method 978.10) and crude ash (AOAC Official method 942.05). Macro and microelements (Ca, P, Mg, K, Na, Zn, Mn, Cu and Fe) were determined after ashing and preparation of an acid extract by atomic absorption spectrometry (1100B, PerkinElmer Inc., Waltham, MA, USA). Each sample was analysed in duplicate.
4
High-Fat Diet in C57BL/6J Mice
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Four-week-old female C57BL/6J mice were purchased at weaning from the Jackson Laboratory (Stock #000664, Bar Harbor, ME, USA). All mice were single-housed and maintained in a conventional animal facility at 21–22°C at a relative humidity of 50±10% on a 12-h light:dark cycle from time of weaning. Mice were fed a diet consisting of 60% kcal from fat, 20% kcal from protein, and 20% kcal from carbohydrate (#D12492, Research Diets, New Brunswick, NJ, USA) or 16% kcal from fat, 20% kcal from protein, and 64% kcal from carbohydrate (#D11112201, Research Diets, New Brunswick, NJ, USA) according to the study design. Animals were given ad libitum access to food (maintaining approximately 50 g of food in the hopper) and water unless indicated. Prior to treatment allocation or experimentation, each animal was weighed weekly and body temperature was recorded via infrared thermometry during weighing (Fluke 572-2 Infrared Thermometer; Fluke, Everett, WA, USA).
5
Trametinib Supplementation in Mice
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Details on animal methods, as well as histology, cell culture, analytical techniques, and statistics are available in the Supplemental Appendix . All experiments on mice were approved by the Institutional Animal Care and Use Committee of the Medical University of South Carolina. A total of 40 mice (1-5 per cage) were kept in a 12-hour light/dark cycle with food and water ad libitum. Control mice received an open-source diet (D11112201) produced by Research Diets. Starting at 2 months of age, experimental mice received the same base diet supplemented with trametinib (10 mg/kg of food). On average, 20-g animals consumed 3 g of food per day, amounting to 1 mg/kg/d of trametinib. No differences in food consumption were noted between groups. All data are presented as mean ± SD. Statistical analysis was performed using Prism 9 (GraphPad Prism Software). For normally distributed data, unpaired data were compared using Student’s t-test or 2-way analysis of variance (ANOVA) with the Bonferroni post hoc test for multiple pairwise comparisons. For nonparametric data, the Mann-Whitney U test was used. Survival was compared using the log-rank test. Values are P < 0.05 were considered to indicate statistical significance.
6
Probiotic Effects on tert-Butyl Hydroperoxide-Induced Oxidative Stress in Mice
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C57BL/6 female mice (5-weeks old) were purchased from Orient Bio Inc. (Gyeonggi-do, Korea) and housed under a 12/12 h light–dark cycle (7 a.m. to 7 p.m.) in a room under controlled conditions (22 ± 3 °C, 50 ± 20% relative humidity, 150–300 Lux). The mice were fed a commercial laboratory diet (D11112201; Research Diets Inc., New Brunswick, NJ, USA) and water ad libitum. All animal experimental protocols used in this study were approved (Approval No. P214054) by the Institutional Animal Care and Use Committee at the NDIC, Gyeonggi-do, Korea.
The treatments have been described previously [22 (link)]. All animals were randomly divided into five groups (n = 7 in each group) and orally administered (p.o.): (1) saline (normal control, NOR), (2) tert-butyl hydroperoxide (t-BHP), (3) Lc. lactis MG5125 (1 × 109 CFU/g/day in saline), (4) B. bifidum MG731 (1 × 109 CFU/g/day in saline), and (5) B. lactis MG741 (1 × 109 CFU/g/day in saline). On day 15, all animals except the NOR group were intraperitoneally (i.p.) injected with t-BHP (0.5 mmol/kg body weight), and the mice were sacrificed after 24 h. Blood was collected to obtain serum samples and stored at 80 °C until further experiments.
The treatments have been described previously [22 (link)]. All animals were randomly divided into five groups (n = 7 in each group) and orally administered (p.o.): (1) saline (normal control, NOR), (2) tert-butyl hydroperoxide (t-BHP), (3) Lc. lactis MG5125 (1 × 109 CFU/g/day in saline), (4) B. bifidum MG731 (1 × 109 CFU/g/day in saline), and (5) B. lactis MG741 (1 × 109 CFU/g/day in saline). On day 15, all animals except the NOR group were intraperitoneally (i.p.) injected with t-BHP (0.5 mmol/kg body weight), and the mice were sacrificed after 24 h. Blood was collected to obtain serum samples and stored at 80 °C until further experiments.
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