Sercadis 30 sc

The intrinsic activity of SDHI molecules against A. alternata and K. variispora was determined in 15 and 10 isolates of A. alternata and K. variispora, respectively. The isolates had been collected during the 2017 survey, and had not been exposed to the selection pressure of SDHIs. SDHI molecules selected for the study were boscalid (Cantus 50 WG, BASF Hellas, Marousi, Greece), fluopyram (Luna Privilege 50 SC, Bayer CropScience Hellas, Athens, Greece), fluxapyroxad (Sercadis 30 SC, BASF Hellas), penthiopyrad (Fontelis 20 SC, Elanco Hellas, Chalandri, Greece), and adepidyn (A19649H 20 SC, experimental formulation kindly provided to us by Syngenta Hellas, Anthoussa, Greece). The assessment of isolates' sensitivity to these active ingredients was based on the inhibition of mycelial growth at different fungicide concentrations (0, 0.01, 0.05, 0.1, 0.5, 1, 5, and 10 μg ml -1 ) based on a protocol developed by Avenot et al. (2008) for A. alternata isolates from pistachio. The EC 50 value for each isolate was calculated by plotting the relative inhibition of mycelial growth against the Log 10 fungicide concentrations in three independent measurements. Calculations were performed using the program JMP (SAS Institute, Cary, NC).