For this study we harvested 8 mm diameter cartilage cores of 1–2 mm thickness from human femoral heads, femoral condyles and tibial plateau removed at surgery. Non-pathological cartilage was obtained from femoral neck fracture patients or vasculopathic patients following transfemoral amputation where there was no history or radiological evidence of arthritis. Osteoarthritic cartilage came from patients undergoing hip or knee replacement for primary osteoarthritis. The tissue samples were vacuum packed for transportation and for ease of manipulation and tissue preservation. A layer of subchondral bone of up to 1 mm was left attached to the core to ensure that the entire thickness of cartilage was being analysed; previous experiments confirmed that this had negligible influence on the results because NMR signal from the subchondral bone is negligible compared to that of cartilage. The cores were placed in NMR tubes with fluorinert (FC-70, Sigma) to avoid evaporation during Fast Field-Cycling NMR analyses. Samples that could not be analysed directly were stored at 4 °C for up to 5 days. This study was approved by the local North of Scotland Ethics Committee (NoSREC, www.nres.npsa.nhs.uk ).
Fc 70
Manufactured by Merck Group
Sourced in Germany
FC-70 is a specialized laboratory equipment used for various applications in research and analytical settings. It serves as a high-performance fluorination reagent, enabling targeted fluorination reactions. The core function of FC-70 is to facilitate the introduction of fluorine atoms into organic compounds, a process crucial for the development of new materials, pharmaceuticals, and other compounds with unique properties.
Automatically generated - may contain errors
Lab products found in correlation
Cerium nitrate ce no3 3, by Merck Group (1 mentions)
3 octanol, by Merck Group (1 mentions)
Fc 40, by Merck Group (1 mentions)
1 2 propanediol, by Merck Group (1 mentions)
Oxalic acid h2c2o4, by Merck Group (1 mentions)
Hexadecane, by Merck Group (1 mentions)
Sylgard 184, by Dow (1 mentions)
Ludox sm, by Merck Group (1 mentions)
Hellmanex solution, by Hellma (1 mentions)
3 protocols using fc 70
1
Harvesting and Analyzing Human Cartilage
2
Microfluidic Synthesis with OSTE+ Polymers
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Off-stoichiometry thiol-ene-epoxy (OSTE+) pre-polymers (OSTEMER 322) are purchased from Mercene Labs. The negative molds and flats were prepared by mixing the PDMS elastomer base with its curing agent (Sylgard 184, DOW). The carrier phases FC-40, FC-70, FC-3283, perfluo-rodecaline, 3-octanol, hexadecane, 1,2-propanediol and light mineral oil were acquired from Sigma-Aldrich. Span 80 (sorbitan monooleate) was acquired from CRODA and used in combination with hexadecane and light mineral oil. The gold nanoparticles suspension was obtained from a classical reverse Turkevitch synthesis (see ESI Section 1 † ). Cerium nitrate (Ce(NO 3 ) 3 ) and oxalic acid (H 2 C 2 O 4 ) were purchased from Sigma-Aldrich and used without further purification to prepare aqueous solutions at 0.10 m Ce(NO 3 ) 3 and 0.15 m H 2 C 2 O 4 .
3
Silane-Based Superhydrophobic Surface Fabrication
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Materials (Tridecafluoro-1,1,2,2-tetrahydrooctyl)-1-trichlorosilane (97%), FC-70 (97%, C15F33N), 1-butyl-2,3-dimethylimidazolium bis(trifluoromethanesulfonyl)imide C 11 H 17 F 6 N 3 O 4 S 2 ethylene glycol, hexadecane, and silica nanoparticles (LUDOX SM) were purchased from Sigma-Aldrich, Germany. All chemicals were used without further purification. Microscope glass slides (Menzel, Germany) with a thickness of 170 mm were cleaned with a 2% Hellmanex solution (Hellma, Germany).
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