Female BALB/c mice (4–6 weeks of age) were purchased from The Jackson Laboratory and housed in pathogen-free conditions on a 12-hour dark/12-hour light cycle at 22 ± 3°C with access to food and water ad libitum. H9C2-derived T. cruzi Y strain tissue culture trypomastigotes to be used for infections of BALB/c mice were first passaged through female SCID mice (The Jackson Laboratory) bloodstream trypomastigotes. SCID mice were infected by i.p. injection of 2 × 103 tissue culture trypomastigotes in 0.2 mL PBS and peripheral blood parasitemia was monitored by analysis of saphenous venous blood (3 μL). When SCID mice parasitemia reached 1 × 108 trypomastigotes/mL, infected blood from SCID mice was harvested and adjusted to 2 × 104 bloodstream trypomastigotes/mL by the addition of PBS. BALB/c mice were then infected with 2 × 103 bloodstream trypomastigotes by i.p. injection. Parasitemia of Y strain–infected mice was determined and animals were euthanized after the final time point.
Female scid mice
Manufactured by Jackson ImmunoResearch
Sourced in Montenegro
Female SCID mice are a laboratory animal model characterized by a severe combined immunodeficiency. They lack functional T and B lymphocytes, making them useful for research applications that require a model with a compromised immune system.
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3 protocols using female scid mice
1
Murine Trypanosoma cruzi Infection Model
2
SCID Mice Experiment Protocol
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Female SCID mice were purchased from the Jackson Laboratory (Bar harbor, ME), female FVB/N mice and BALB/c wild-type mice were purchased from Charles River Laboratories (Wilmington, MA). All the animals used in this study were 8–12 weeks old. At least five mice per experiment were used for this study. Mice were housed in the Kansas City Veteran Administration Medical Center (KCVAMC) animal care facility. Mice were fed commercial mouse diet food (Envigo, Indianapolis, IN) with a 12-hour light-dark cycle under pathogen-free conditions. Experimental animals were carefully monitored throughout the course of the experiment and if they showed signs of distress including, but not limited to, reduced food or water intake, reduced activities, hunched posture, weight loss, vocalization, irritability or lack of grooming, they were euthanized. Euthanasia was performed using CO2 inhalation followed by cervical dislocation.
3
Metastatic Colon Cancer in SCID Mice
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Female SCID mice (strain no: 001303, RRID:IMSR-JAX:001303; 6 weeks) purchased from Jackson Laboratory were used in these studies, and all protocols were approved by the University of Pittsburgh Committee on Animal Care and Use Committee, and experiments were carried out in adherence to the NIH Guidelines for the Use of Laboratory Animals. Animals were raised in plastic cages under specific pathogen-free conditions. Animals were fed a standard diet and had free access to water in an animal facility of the University of Pittsburgh. Mice were anesthetized with isoflurane. SW480-shiNOS2 or SW480-shControl cells (2 × 106 cells/mouse) in PBS, were injected into spleen or inner canthus. The mice were sacrificed 4-week post-injections. The organs were collected, frozen with OCT, and 5-μm sections cut for hematoxylin and eosin (H&E) stains. Established metastatic colon and rectal tumors in lymph nodes, stomachs, and other organs as previously described (18 (link)).
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