Dihydrocatalpol (DCAT), melittoside (MEL), genipin (GE), geniposide (GEN), geniposidic acid (GPA), rehmannioside A (RA), and rehmannioside D (RD) were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Catalpol (CAT), aucubin (AU), ajugol (AJU), and rehmannioside C (RC) were purchased from Sichuan Victory Biological Technology Co., Ltd. (Sichuan, China). Corticosterone (CORT) was purchased from Cayman Chemical company (Ann Arbor, MI, USA). Fluoxetine (FLX) was a positive drug purchased from MedChemExpress (Monmouth Junction, NJ, USA). RPMI medium 1640 was obtained from Gibco (Grand Island, NY, USA). Fetal bovine serum (FBS) was obtained from Zhejiang Tianhang Bio-Technology Co., Ltd. (Zhejiang, China). HPLC-grade acetonitrile and methanol were supplied by Fisher Chemical (Waltham, MA, USA). Formic acid was supplied by Fisher Scientific (Waltham, MA, USA). Ultrapure water was purified with Arium® Pro Ultrapure Water Systems (Sartorius Stedim Biotech, Göttingen, Germany).
Geniposide
Manufactured by Yuanye Bio-Technology
Sourced in China
Geniposide is a chemical compound extracted from the fruit of the Gardenia jasminoides plant. It is a key ingredient used in various laboratory applications. Geniposide serves as a standard reference material for analytical and quality control purposes in research and development settings.
Automatically generated - may contain errors
Lab products found in correlation
Geniposidic acid, by Yuanye Bio-Technology (3 mentions)
Formic acid, by Thermo Fisher Scientific (2 mentions)
Genipin, by Yuanye Bio-Technology (2 mentions)
Genipin 1 gentiobioside, by Yuanye Bio-Technology (2 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Arium pro ultrapure water system, by Sartorius (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Hplc grade acetonitrile, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Zhejiang Tianhang Biotechnology (1 mentions)
Waters 2695 hplc instrument, by Waters Corporation (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Gsh kit, by Nanjing Jiancheng (1 mentions)
Dapi staining solution, by Solarbio (1 mentions)
Crocetin, by Yuanye Bio-Technology (1 mentions)
Chlorogenic acid, by Yuanye Bio-Technology (1 mentions)
Hyperoside, by Yuanye Bio-Technology (1 mentions)
Corticosterone, by Tokyo Chemical Industry (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
6 protocols using geniposide
1
Bioactive Compounds Evaluation Protocol
2
HPLC Analysis of Geniposide and Genipin
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Sterilized geniposide and genipin standards (Yuanye Biological Technology Co., Ltd., 2 mg/mL) were prepared in 10 mL volumetric flasks, respectively. The liquid chromatography experiment was performed under the following conditions: C18 chromatographic column (2.1 mm × 50 mm, 1.7 μm, Elite HPLC, Dalian, Liaoning, China); the column temperature should be maintained at 25 °C; the mobile phase was 35–65% methanol-water; the flow rate was 1 mL/min; the detection wavelength was 238 nm; and the volume of the cell treatment was 2 μL (geniposide group, 2 mg/mL; geniposide and LcS mixture group).
3
HPLC Analysis of Iridoid Glycosides
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The HPLC separation was performed using a Waters WATO54275-C18 column (250x4.6 mm, 5 µm, Waters China, Ltd.) installed on a Waters 2695 HPLC instrument (Waters Corporation). The mobile phase consisted of 0.1% phosphoric acid (A) and acetonitrile (B) with a gradient elution: 0-15 min, 4-25% B; 15-20 min, 25-28% B; 20-40 min, 28-38% B; 40-41 min, 95% B; 41-51 min, 95-4% B. The flow rate was 1 ml/min, and the injection volume was 10 µl. The temperature of the column was set at 25˚C. Standard compounds, including geniposidic acid, deacetylasperulosidic acid, genipin-1-gentiobioside, geniposide, crocin I and II with >98% purity were purchased from Shanghai Yuanye Bio-Technology Co., Ltd.
4
Hepatoprotective Compounds Evaluation
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Standard compounds geniposide, gardenoside, genipin-1-gentiobioside, shanzhiside, chlorogenicacid, crocin-1, crocin-2, crocetin, geniposidic acid, and genipin with over 98% purity were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Cell Counting Kit-8 purchased from EnoGene technology Co., Ltd. (Nanjing, China). Annexin V-FITC/PI apoptosis detection kit purchased from BestBio technology Co., Ltd. (Shanghai, China). DAPI staining solution purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). TNF-α,IL-6, and NO enzyme-linked immuno sorbent assay (ELISA) kits (R&D, USA) were purchased from Changchun Bestgene Biotechnology Co., Ltd. (Changchun, China). ALT, AST, ALP, MDA, SOD, and GSH kits were obtained from Nanjing JianCheng Institute of Biological Engineering (Nanjing, China). Acetonitrile and Formic acid (Fisher, Fair Lawn, NJ, USA) were of HPLC grade.
5
Quantitative Analysis of Hyperoside and Geniposide
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The reference standards of hyperoside (≥ 98% pure) and geniposide (≥ 98% pure, internal standard) were purchased from Shanghai Yuanye Bio-Technology Co. Ltd. (Shanghai, China); their chemical structures are shown in Fig. 1 . Methanol of HPLC grade were obtained from Merck KGaA (Darmstadt, Germany). Acetic acid glacialof HPLC grade was obtained from Tedia company (USA). Ultrapure water was purified by an GenPure UV-TOC/UF xCAD plus (Thermo Electrion LED GmbH, Germany). Other reagents and chemicals were of analytical grade.![]()
Chemical structures of hyperoside (
6
Evaluation of Neuroprotective Compounds
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Corticosterone was obtained from Tokyo Chemical Industry (TCI) Development Co., Ltd. (Shanghai); Fetal bovine serum (FBS) was obtained from Biological Industries; LDH Assay kit was purchased from Nanjing Jiancheng Bioengineering Institute; Annexin V-FITC/PI kit was purchased from Jiangsu Kaiji Biotechnology Co., Ltd. and MTT was obtained from Sigma-Aldrich.
Fluoxetine hydrochloride capsules (batch number: 5548C) were purchased from Lilly Suzhou Pharmaceutical Co., Ltd; Genipin 1-gentiobiside (batch number: B20637, purity ≥98%) was obtained from Shanghai Yuanye Biotechnology Co., Ltd. Geniposide (batch number: MUST-17020401, purity ≥98%), naringin (batch number: MUST-17040102, purity ≥98%), hesperidin (batch number: MUST-17032502, purity ≥98%), neohesperidin (batch number: MUST-17040707, purity ≥98%), honokiol (batch number: MUST-17020205, purity ≥98%) and magnolol (batch number: MUST- 18032102, purity ≥98%) were purchased from Chengdu Munster Biotechnology Co., Ltd. Methanol and acetonitrile are purchased from Merck. The ultrapure water for was prepared by a Milli-Q purification instrument. All other chemical reagents are of analytical grade.
Fluoxetine hydrochloride capsules (batch number: 5548C) were purchased from Lilly Suzhou Pharmaceutical Co., Ltd; Genipin 1-gentiobiside (batch number: B20637, purity ≥98%) was obtained from Shanghai Yuanye Biotechnology Co., Ltd. Geniposide (batch number: MUST-17020401, purity ≥98%), naringin (batch number: MUST-17040102, purity ≥98%), hesperidin (batch number: MUST-17032502, purity ≥98%), neohesperidin (batch number: MUST-17040707, purity ≥98%), honokiol (batch number: MUST-17020205, purity ≥98%) and magnolol (batch number: MUST- 18032102, purity ≥98%) were purchased from Chengdu Munster Biotechnology Co., Ltd. Methanol and acetonitrile are purchased from Merck. The ultrapure water for was prepared by a Milli-Q purification instrument. All other chemical reagents are of analytical grade.
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