Vero ccl 81 cell line

The human pancreatic cancer cell lines PANC-1 (CRL-1469), AsPC-1 (CRL-1682), BxPC-3 (CRL-1687) and MIA PaCa-2 (CRL-1420) cell lines as well as the Vero (CCL-81) cell line derived from the kidney of a normal adult African green monkey were obtained from ATCC (Manassas, VA), grown, aliquotted and maintained in liquid nitrogen. Aliquots of AsPC-1, PANC-1, Vero and BxPC-3 were thawed and grown in RPMI-1640 (ATCC, Manassas, VA) supplemented with 10% Fetal Bovine Serum (Hyclone, Logan, UT), 0.11mg/ml Sodium pyruvate, 4.5g/L D-glucose, 18mM HEPES Buffer, 100U/ml penicillin G sodium, 100μg/ml streptomycin sulfate, 0.25μg/ml amphotericin B, 2mM L-glutamine and 50μg/ml gentamicin (Complete RPMI). Sodium pyruvate and glucose were purchased from Sigma, St Louis, MO. All other supplements were purchased from Gibco, Carlsbad, CA. The MIA PaCa-2 cell line was grown in DMEM media (ATCC, Manassas, VA) supplemented with 5% horse sera (ATCC, Manassas, VA), 10% Fetal Bovine Serum (Hyclone, Logan, UT), 100U/ml penicillin G sodium, 100μg/ml streptomycin sulfate, 0.25μg/ml amphotericin B and 50μg/ml gentamicin. Cells were maintained in a humidified incubator at 37°C and 5% CO₂.