Annexin-V FITC and PI double staining kit (Invitrogen, Grand Island, NY, USA) was used to analyze ZOL-induced cell apoptosis. After being cultured with medium containing ZOL at 0 μM, 1 μM, 10 μM, 100 μM, 200 μM, and 300 μM for 24 h, CT26 cells were collected and resuspended with 1 mL 1x binding buffer solution. After being fixed with 4% paraformaldehyde, 5 μL of annexin-V FITC and 500 μL of PI were added to each tube, mixed well, and incubated in dark for 15 min. The cells were finally resuspended with 400 μL of 1x binding buffer. The apoptotic cells were detected with flow cytometry (BD Biosciences, San Jose, CA, USA).
Annexin 5 fitc and pi double staining kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Annexin-V FITC and PI double staining kit is a laboratory reagent used for the detection and quantification of apoptosis in cell samples. The kit contains Annexin-V conjugated with the fluorescent dye FITC and the dye propidium iodide (PI). Annexin-V binds to phosphatidylserine, which is exposed on the surface of apoptotic cells, while PI stains the DNA of cells with compromised cell membranes. This allows for the identification and differentiation of viable, early apoptotic, and late apoptotic/necrotic cells using flow cytometry or fluorescence microscopy.
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2 protocols using annexin 5 fitc and pi double staining kit
1
Apoptosis Induction by Zoledronic Acid
2
Annexin V-FITC and PI Apoptosis Assay
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Annexin V-FITC and PI double staining kit (Invitrogen, Grand Island, NY, USA) was applied to analyze Spica Prunellae-induced cell apoptosis. After being cultured with medium containing the Spica Prunellae at 0, 10, 50, 100, 500, and 1000 mg/L for 24 h, A549 cells were collected and resuspended with 1 mL 1× binding buffer solution. After being fixed with 4% paraformaldehyde, 5 μL of annexin V-FITC and 500 μL of PI were added to each tube, mixed well, and incubated in dark for 15 min. The cells were finally resuspended with 400 μL 1× binding buffer. The apoptosis of cells was detected with flow cytometry (BD Biosciences, San Jose, CA, USA).
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