Antibody against caspase 3

Dulbecco’s modified Eagles medium with Hams F-12 nutrient mixture (DMEM/F12, 1:1) was purchased from Hyclone (South Logan, UT, USA). Fetal bovine serum (FBS) was obtained from ZETA Life (Menlo Park, CA, USA). The antibody against β-actin was purchased from Tianjin Sungene Biotechnology Inc. (Tianjin, China). The antibodies against ATF-6, P-IRE1α, EIF2S1, P-EIF2S1, ATF-4, GRP78, and LC3I/II were purchased from Abcam (Cambridge, MA, USA). The antibody against caspase-3 was obtained from Abclonal (Wuhan, China). Antibodies against poly (ADP-ribose) polymerase 1 (PARP-1) and Bax were purchased from Proteintech (Wuhan, China). Likewise, 3-Methyladenine (3-MA), chloroquine (CQ), and 4-phenylbutyric acid (4-PBA) were obtained from Sigma-Aldrich (St. Louis, MO, USA). The LDH cytotoxicity assay kit was purchased from Promega (Madison, WI, USA).

The Sf9 cells were cultured in Sf-900 II SFM medium (Gibco, 10902088, Grand Island, NY, USA) supplemented with 6% FBS (GIBCO, Grand Island, NY, USA), at 28 ℃. RIPA buffer (#9806S), and antibodies against Bax (#2772), Bcl-2 (#15071), caspase-9 (#9508), cytochrome c (#11940), and β-actin (#8457) were obtained from Cell Signaling Technology (Beverly, MA, USA). Acridine orange (#A8120) was purchased from Solarbio Life Science (Beijing, China). Antibody against Bcl-XL (#abs131907) was purchased from Absin Bioscience (Shanghai, China). Antibody against Caspase-3 (#A2156) was purchased from ABclonal (Wuhan, China). Goat anti-mouse IgG-HRP conjugate (#sc-2005) or anti-rabbit (#sc-2357) was purchased from Santa Cruz (Santa Cruz, TX, USA). DCFH-DA (#S0033) and Rhodamine123 (#C2007) were purchased from Beyotime Biotechnology (Nanjing, China). LysoSensor™ Green DND-189 (#40767ES50) was purchased from Yeasen Biotechnology (Shanghai, China).