Rmv 707b 30 mhz probe

CADcre (VE-cadherin-driven Cre mice backcrossed into the C57BL/6 background, N = 6) and ECK4KO (floxed Klf4 mated to CADcre, N = 9), male, 5–7 months old after bone marrow transplantation for 8 weeks were used for this experiment. Mice were anesthetized with Avertin (0.25 mg/g, i.p.) and restricted on a temperature-controlled small animal table to maintain physiological body temperature during experiment, a jugular vein catheter was used for continuous acetylcholine infusion. A Vevo 770 High-Resolution Imaging System equipped with an RMV-707B 30-MHz probe (VisualSonics) was used for echocardiography. M-mode sampling was used through the upper abdominal aorta long axis, images were recorded under baseline and then after continuous acetylcholine infusion (2 μg/kg/min, 10 μl/min, Sigma-Aldrich, St Louis, MO) for the indicated time intervals. The abdominal inner diameter at end-systolic and end-diastolic were analyzed through abdominal aorta M-mode image.

Mice were anesthetized with Avertin (0.25 mg/g i.p.), and transthoracic echocardiography was performed using a Vevo 770 High-Resolution Imaging System equipped with an RMV-707B 30-MHz probe (VisualSonics). Standard M-mode sampling was used at the ascending aorta, and artery diameter at systole and diastole determined using the system software. Distensibility was calculated using the following formula: distensibility = (systolic diameter−diastolic diameter)/diastolic diameter × 100%. Control (C57BL/6 J) at 3 and 10–14 months old and EC-KLF4 Tg male mice at 3 and 10–12 months old were used for vessel compliance analysis by echocardiogram of ascending aorta. Experiments were performed in a nonblinded manner.