Equal amounts of protein (30 μg) were separated by electrophoresis in NuPAGE Bis-Tris Gels (Life Technologies) and transferred to nitrocellulose membranes. The membranes were hybridized with various primary antibodies (APP 6E10, 1/500, Covance; PS1, 1/1000, Millipore; APP C-ter, 1/500, Millipore; Actin, 1/2000, Abcam; GAPDH, 1/1000 Abcam; Total Tau, 1/1000, Santa Cruz; PSD-95, Invitrogen, 1/2000; Synaptophysin, Santa Cruz, 1/200; GAD65, Abcam, 1/2000; GLT-1, 1/1000, Frontier Science; GLAST, 1/1000, Frontier Science; NeuN, Millipore, 1/1000). Various secondary antibodies was also used (ECL Anti-rabbit Horseradish Peroxidase linked, 1/2000, GE Healthcare; ECL Anti-mouse Horseradish Peroxidase linked, 1/2000, GE Healthcare; ECL Anti-rat Horseradish Peroxidase linked, 1/2000, GE Healthcare).
App c ter
Manufactured by Merck Group
The APP C-ter is a laboratory equipment product developed by Merck Group. It is designed to perform a specific function within the laboratory setting. The core function of the APP C-ter is to facilitate a particular aspect of laboratory operations. No further details or interpretations are provided, as doing so would go beyond the scope of a concise, factual, and unbiased description.
Automatically generated - may contain errors
Lab products found in correlation
Nupage bis tris gel, by Thermo Fisher Scientific (2 mentions)
App 6e10, by Fortrea (2 mentions)
Gapdh, by Abcam (2 mentions)
Synaptophysin, by Santa Cruz Biotechnology (1 mentions)
Total tau, by Santa Cruz Biotechnology (1 mentions)
Psd 95, by Thermo Fisher Scientific (1 mentions)
Gad65, by Abcam (1 mentions)
Ecl anti rabbit horseradish peroxidase linked, by GE Healthcare (1 mentions)
Neuronal nuclei (neun), by Merck Group (1 mentions)
Eclipse ti microscope, by Leica (1 mentions)
4g8 biotin, by Fortrea (1 mentions)
3 protocols using app c ter
1
Western Blot Analysis of Neurological Proteins
2
Immunostaining of Cryosectioned Samples
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Cryosections were washed with 0.25 % Triton in PBS and saturated by incubation (0.25 % Triton in PBS/5 % goat serum). They were then incubated with primary antibodies (APP C-ter, 1/500, Millipore; NeuN-Biotin, 1/1000, Millipore; 4G8-Biotin, 1/1000, Covance). Images were taken with a Nikon Eclipse Ti Microscope or a Leica TCS SP8 confocal microscope and analyzed with ImageJ software (NIH).
3
Alzheimer's Disease Molecular Markers
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Equal amounts of protein (30 µg) were separated by electrophoresis in NuPAGE Bis-Tris Gels (Life Technologies) and transferred to nitrocellulose membranes. The membranes were hybridized with various primary antibodies: APP 6E10 (1/500, Covance), PS1 (1/1000, Millipore), APP C-ter (1/500, Millipore), GAPDH (1/1000, Abcam), P-tau Thr 212 (1/1000, Invitrogen), P-tau Thr 422 (1/1000, Invitrogen), P-CamkII Thr 286 (1/1000, Abcam). Various secondary antibodies were also used: ECL horseradish peroxidase linked antirabbit, ECL horseradish peroxidase linked Anti-mouse, ECL horseradish peroxidase linked anti-rat (all 1/2000, GE Healthcare).
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