Dulbecco modified eagles medium

The antibodies for KLF16 and PPARγ was purchased from Abcam (Cambridge, MA). Insulin, T3, indomethacin, isobutylmethylxanthine (IBMX) and dexamethasone were purchased from Sigma Aldrich (St. Louis, MO). Dulbecco’ Modified Eagles’ Medium (DMEM), fetal bovine serum (FBS), and penicillin/streptomycin were purchased from Life Technologies (Grand Island, NY)

Tricarballylic acid (TCA), 1,10-decanediol, stannous 2-ethylhexanoate, Triethyl amine (TEA), 2,2-dimethoxy-2-phenylacetophenone (DMPA) and Polyvinylpyrrolidone (PVP), absolute Ethanol, 4-dimethylamino Pyridine (DMAP), and phenolphthalein were all purchased from Sigma-Aldrich Chemie GmbH, Taufkirchen, Germany. Acryloyl chloride (ACRL) Lichrosolv^® Acetone, Acetic anhydride, Pyridine, Hydrochloric acid (37%), and Chloroform were purchased from Merck, Kenilworth, NJ, USA. All chemicals were used as received without any further purification. Fetal bovine serum, l-Glutamine 200 mM (100×), 2-Mercaptoethanol (50 mM), and the Live/Dead Calcein-AM^® Viability/Cytotoxicity assay kit for mammalian cells, Dulbecco modified eagle’s medium (DMEM), and the Gibco^® F-12 nutrient mixture were purchased from Life Technologies Co., Paisley, UK. Penicillin-streptomycin and Dulbecco’s phosphate buffered saline (DPBS) were purchased from Sigma-Aldrich Chemie GmbH, Taufkirchen, Germany; DAPI (4′,6-diamidino-2-phenylindole) for nucleic acid staining was purchased from Thermo-Fisher scientific, Waltham, MA, USA. Lonza Trypsin/EDTA (10×) was purchased from SLS life sciences Co., Warwick, UK. They were all used without further purification.

SPEA SAg gene with accession number (KY594414) was synthesised by Geneart GmbH (Regensburg, Germany) and previously cloned and expressed in E. coli. SPEA-based peptides were synthesized and supplied by GenScript (Piscataway, NJ, USA). MDA-MB-468 cell line was obtained from ATCC collections, MDA-MB-468 (ATCC^® HTB-132™). Fetal bovine serum, L-Glutamine 200 mM (100×), and Dulbecco modified eagle’s medium (DMEM) were purchased from Life Technologies Co., Paisley, UK. Penicillin-streptomycin and Dulbecco’s phosphate buffered saline (DPBS) were purchased from Sigma-Aldrich Chemie GmbH, Taufkirchen, Germany; DAPI (40′,6-diamidino-2-phenylindole) for nucleic acid staining was purchased from Thermo-Fisher Scientific, Waltham, MA, USA. Lonza Trypsin/EDTA (10×) was purchased from SLS life sciences Co., Warwick, UK. Custom ProcartaPlex Multiplex Panel targeting IL-1β, IL-2, IL-6, IFNγ, TNFα, and IL-10 was purchased from Thermo-Fisher Scientific. Human anti-CD3-APC IgG1 and anti-CD25-FITC were purchased from ImmunoTools (Friesoythe, Germany). Anti-EGFR antibody [EP38Y] and Goat Anti-Rabbit IgG H&L (FITC) were from Abcam (Cambridge, UK).