Upa elisa kit

Manufactured by Abcam

Sourced in United Kingdom

The UPA ELISA kit is a quantitative enzyme-linked immunosorbent assay (ELISA) designed to measure the levels of urokinase-type plasminogen activator (uPA) in biological samples. This kit provides a reliable and standardized method for the detection and quantification of uPA, which is an important serine protease involved in various physiological and pathological processes.

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Lab products found in correlation

Sta r evolution, by Diagnostica Stago (2 mentions) Asserachrom free tfpi enzyme immunoassay, by Diagnostica Stago (2 mentions) Zymutest pai 1 activity, by Hyphen Biomed (2 mentions) Cellquest pro software 5, by BD (1 mentions) Facscan, by BD (1 mentions)

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ELISAs (10 citations) Mouse uPA ELISA Kit (2 citations)

3 protocols using upa elisa kit

Coagulation and Fibrinolysis Profiles in Warfarin-Treated Families

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Several coagulation and fibrinolytic parameters, not belonging to the classical thrombophilia screening, were measured in 10 family members with plasma available (3 cases and 7 controls). The 3 cases were on warfarin whereas none of the controls were on anticoagulant treatment at the time of the sampling.
Activated partial thrombin time (aPTT) and prothrombin time (PT), fibrinogen, factor II, factor V and factor X were measured in plasma with automated coagulometers (STA-Revolution, Diagnostica Stago, Asnières, France) using established commercial assays. Free tissue factor pathway inhibitor (f-TFPI) plasma levels were measured with the Asserachrom Free TFPI enzyme immunoassay from Diagnostica Stago (Asnieres, France) and plasma PAI-1 activity with the Zymutest PAI-1 activity from Hyphen Biomed (Neuville-sur-Oise, France). Urokinase (uPA) plasma levels were measured with the uPA ELISA kit (Abcam, Cambrige, UK). The euglobulin clot lysis time (ECLT) assay has been performed as previously described [29 (link)].

Morange P.E., Peiretti F., Gourhant L., Proust C., Soukarieh O., Pulcrano-Nicolas A.S., Saripella G.V., Stefanucci L., Lacroix R., Ibrahim-Kosta M., Lemarié C.A., Frontini M., Alessi M.C., Trégouët D.A, & Couturaud F. (2021). A rare coding mutation in the MAST2 gene causes venous thrombosis in a French family with unexplained thrombophilia: The Breizh MAST2 Arg89Gln variant. PLoS Genetics, 17(1), e1009284.

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Extracellular uPA and uPAR Detection

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The serum-free supernatant from the EC-109, MCF-7 and SK-BR-3 cell cultures was collected and centrifuged at 850 × g for 10 min at 4°C (ThermoHeraeus Multifuge X1R; Thermo Fisher Scientific, Inc.) for detection of uPA (three samples from each cell line). Triple wells were used in each cell line. An ELISA was performed to determine the level of uPA secreted from the cells using an uPA ELISA kit (catalog no., ab108917; Abcam, Cambridge, MA, USA) The assay was performed according to the manufacturer's instructions. The extracellular level of uPAR was determined using a FACscan flow cytometer (BD Biosciences, San Jose, CA, USA) (3 samples in each cell line). The procedure is briefly described, as follows: 1×10⁶ EC-109, MCF-7 and SK-BR-3 cells were incubated with an anti-uPAR antibody (10 µg per 1×10⁶ cells) (catalog no., sc-13522; Santa Cruz Biotechnologies, Dallas, USA) for 2 h at 4°C. The cells were washed three times with PBS containing 0.05% Tween-20 to remove the unbound antibody and then incubated with a fluorescein isothiocyanate-conjugated secondary antibody (dilution, 1:500; catalog no., ab6785; Abcam) for 30 min at room temperature. The normal mouse IgG (cat. no., sc-2025; Santa Cruz) were used as the controls. The flow cytometry results were analyzed using a FACscan instrument (CellQuest Pro Software 5.1; BD Biosciences).

Zhou H., Wang H., Yu G., Wang Z., Zheng X., Duan H, & Sun J. (2017). Synergistic inhibitory effects of an engineered antibody-like molecule ATF-Fc and trastuzumab on tumor growth and invasion in a human breast cancer xenograft mouse model. Oncology Letters, 14(5), 5189-5196.

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Coagulation and Fibrinolytic Factors in Warfarin-Treated Families

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Morange P., Peiretti F., Gourhant L., Proust C., Sapirella G., Pulcrano-Nicolas A., Stefanucci L., Lacroix R., Ibrahim‐Kosta M., Lemarié C.A., Frontini M., Alessi M., Trégouët D., & Couturaud F. (2020). A rare coding mutation in the MAST2 gene causes venous thrombosis in a French family with unexplained thrombophilia: The Breizh MAST2 Arg89Gln variant.

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