Anti mitofusin 2

Antibodies were obtained from the indicated suppliers (Appendix Fig S4): anti‐actin (Abcam), anti‐Bap31 (Abcam), anti‐calnexin (Abcam), anti‐DRP1 (Abcam), anti‐Fis1 (Merck Millipore), anti‐GRP75 (Abcam), anti‐IP3R (Abcam), anti‐LC3 (MBL, Nagoya, Aichi 460‐0008, Japan, Merck Millipore, Burlington, MA, USA), anti‐LRRK2 (Abcam), anti‐MARCH5 (Abcam), anti‐mitofusin 1 (Abcam), anti‐mitofusin 2 (Abcam), anti‐MULAN (United State Biological), anti‐Parkin (Abcam), anti‐PTPIP51 (Abcam), anti‐p62 (MBL), anti‐PERK (Abcam), anti‐VAPB (Abcam), anti‐VDAC1(Abcam), anti‐FLAG (Sigma‐Aldrich), anti‐HA (MBL), anti‐Myc (MBL), anti‐phosphoserine (Abcam), and anti‐V5 (Thermo Fisher). Transfected cells were incubated for 2 days, harvested, and then lysed in lysis buffer (50 mM Tris–HCl [pH 8.0], 150 mM NaCl, and 0.1% SDS) for immunoblotting or in TNE buffer (50 mM Tris–HCl, pH 7.4 150 mM NaCl, 5 mM EDTA, 1% NP‐40, 0.25% Na‐deoxycholate, and 1 mM NaF) for immunoprecipitation/immunoblotting. Immunoprecipitation/immunoblot analyses were performed using standard protocols. The specificity of anti‐phospho‐serine antibody to phosphorylated serine was confirmed by immunoblotting of E3 ubiquitin ligases extracted from transfected cells treated with or without phosphatase (Appendix Fig S5A).