Envison hrp anti mouse igg

Manufactured by Agilent Technologies

Sourced in Denmark

The Envision+ HRP anti-mouse IgG is a secondary antibody reagent used in immunoassays. It is conjugated with horseradish peroxidase (HRP) enzyme, which can be used to detect and amplify the signal from primary antibodies that recognize mouse immunoglobulin G (IgG).

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HRP anti-mouse (6 citations) Envison™ + HRP (2 citations) Anti-TM (1 citations)

2 protocols using envison hrp anti mouse igg

Immunohistochemical Analysis of NODAL and α-SMA in TNBC

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NODAL and α-SMA staining in TNBC tissues was performed as previously described for NODAL [45] (link). Briefly, tissue sections were deparaffinized in xylene and hydrated in graded ethanol. Antigen retrieval was performed with citrate buffer pH 6.0, followed by peroxidase and serum-free protein blocking. After incubation with primary antibodies (Supplementary Table S8), slides were rinsed in TBS-T and treated with Envison+ HRP anti-mouse IgG (Dako, Glostrup, Denmark). Color was produced with 3,3′-Diaminobenzidine (DAB) substrate and counterstained with Mayer's haematoxylin. Samples were dehydrated in graded alcohol and cover slipped with permanent mounting medium.

Dieters-Castator D., Dantonio P.M., Piaseczny M., Zhang G., Liu J., Kuljanin M., Sherman S., Jewer M., Quesnel K., Kang E.Y., Köbel M., Siegers G.M., Leask A., Hess D., Lajoie G, & Postovit L.M. (2021). Embryonic protein NODAL regulates the breast tumor microenvironment by reprogramming cancer-derived secretomes. Neoplasia (New York, N.Y.), 23(4), 375-390.

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Immunohistochemistry for NODAL, CA9, and Stress Markers

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Formalin-fixed, paraffin-embedded tissue underwent deparaffinization in xylenes, hydration through an ethanol series, antigen retrieval with citrate buffer, and peroxidase and serum-free protein blocking. NODAL, CA9, p4E-BP1, 4E-BP1, or ATF4 specific antibodies (Supplementary Table 2) were applied. Slides were rinsed in TBS-T, and treated with Envison+ HRP anti-mouse IgG (Dako). Color was produced with DAB (brown) substrate and counterstained with Mayer’s haematoxylin. Samples were dehydrated in reagent grade alcohol and cover slipped with permanent mounting medium. Negative control reactions were conducted with mouse IgG, isotype controls used at the same concentration as the primary antibodies.

Jewer M., Lee L., Leibovitch M., Zhang G., Liu J., Findlay S.D., Vincent K.M., Tandoc K., Dieters-Castator D., Quail D.F., Dutta I., Coatham M., Xu Z., Puri A., Guan B.J., Hatzoglou M., Brumwell A., Uniacke J., Patsis C., Koromilas A., Schueler J., Siegers G.M., Topisirovic I, & Postovit L.M. (2020). Translational control of breast cancer plasticity. Nature Communications, 11, 2498.

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