Met cys deficient dulbecco s modified eagle s medium dmem

Lipofectamine 2000, Lipofectamine 3000, and 4–12% NuPage gel were purchased from Invitrogen (Carlsbad, CA, USA). Protein A-Agarose was from Santa Cruz Biotechnology (Dallas, TX, USA). Guinea pig anti-insulin was from Merck Millipore (Billerica, MA, USA). Rabbit anti-Human proinsulin was from Biogot (Nanjing, China). Rabbit anti-Myc antibody was from Immunology Consultants Laboratories (Portland, OR, USA). Mouse anti-Hsp90 and anti-GFP antibody were from Sungene Biotech (Tianjin, China). Goat anti-guinea pig IgG Alexa Fluor 488 and goat anti-rabbit IgG Alexa Fluor 555 was bought from Invitrogen (Carlsbad, CA, USA). Enhanced chemiluminescence Western blotting substrate was from Millipore (Billerica, MA, USA). Trans³⁵S label and pure ³⁵S-methionine were from PerkinElmer (Waltham, MA, USA). Met/Cys-deficient Dulbecco’s modified Eagle’s medium (DMEM) was from Invitrogen (Thermo Fisher, Waltham, MA, USA).