P jun s63

Manufactured by Cell Signaling Technology

The P-Jun S63 is a laboratory reagent produced by Cell Signaling Technology. It is an antibody that specifically detects the phosphorylation of the c-Jun protein at serine 63. This phosphorylation event is known to be involved in the regulation of c-Jun transcriptional activity, but the precise functional implications may vary depending on the specific biological context.

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P-JUN (10 citations)

2 protocols using p jun s63

LUAD Tissue and Cell Line Protocol

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Fresh resected human LUAD and matched adjacent noncancerous tissue were obtained after informed consent and approval by the institutional review board (16-1514, 16-107, and 12-245) at Memorial Sloan Kettering Cancer Center. Human LUAD cell lines, human lung fibroblast cells and a normal bronchial epithelial cell line (NL20) were obtained from American Type Culture Collection; Lenti-X 293T cells were purchased from Takara Bio. All cells were tested for mycoplasma. The primary antibodies used were BRMS1 (Abcam, ab134968), c-fos (Cell Signaling, #2250), c-Jun (Cell Signaling, #9165), V5-epitope (Abcam, ab15828), CEACAM6 (Santa Cruz, SC-59899), Src (Cell Signaling, #2109), p-Src Y416 (Cell Signaling, #59548), p-fos S32 (Cell Signaling, #5248), actin (Santa Cruz, sc-8432), p-Jun S63 (Cell Signaling, #2361), RAMP1 (Thermo Fisher, 10327-1-AP), and F5 (Thermo Fisher, PA5-103046). The reagents used—c-fos inhibitor T5224 (S8966) and Src inhibitor Saracatinib (AZD0530, S1006)—were purchased from Selleck Chemicals. Collagen Type IV (C6745), Collagen Type I solution (C3867), and TCN (T7760) were purchased from Millipore Sigma; puromycin (A1113803) and blasticidin (A1113903) were purchased from Thermo Fisher; CellTiter-Glo was obtained from Promega (G9241); and poly-HEMA solution was purchased from Sigma-Aldrich (P3932).

Liu Y., Chudgar N., Mastrogiacomo B., He D., Lankadasari M.B., Bapat S., Jones G.D., Sanchez-Vega F., Tan K.S., Schultz N., Mukherjee S., Offit K., Bao Y., Bott M.J., Rekhtman N., Adusumilli P.S., Li B.T., Mayo M.W, & Jones D.R. (2022). A germline SNP in BRMS1 predisposes patients with lung adenocarcinoma to metastasis and can be ameliorated by targeting c-fos. Science translational medicine, 14(665), eabo1050.

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Rigosertib and Cisplatin Cytotoxicity in HNSCC

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FaDu and Detroit 562 cell lines were purchased from American Type Culture Collection (ATCC, Manassas, VA) and cultured per ATCC recommendations. HPV(−) cell line UMSCC 1 and HPV(+) cell lines UMSCC 47 and UMSCC 104 were kindly provided by the laboratory of Dr. Thomas Carey, University of Michigan. Cells were maintained at 37°C in a humidified, 5% CO₂ atmosphere and cultured in Dulbecco's Modified Eagle's medium (DMEM), supplemented with 10% FBS, 1% penicillin and streptomycin, and MEM non-essential amino acids (Gibco™, Life Sciences Solutions Group, Thermo Fisher Scientific, Carlsbad, CA). Rigosertib (ON 01910.Na) and ON 01911.Na (an inactive control analog) (Figure 1) were provided by Onconova Therapeutics, Inc. (Newtown, PA). These compounds were dissolved in DMSO. Cisplatin was purchased from Sigma-Aldrich (St. Louis, MO). Antibodies to cleaved PARP, cleaved Caspase-3, cleaved Caspase-9, p-mTOR, p-Akt (Ser 473), Akt, p-MSK-1 (T581), p-Erk1/2, Erk1/2, JNK, p-JNK, p-ATF-2 (T71), ATF-2, p-Jun (S63) and COX IV were purchased from Cell Signaling Technology (Danvers, MA). Antibodies to Mcl-1, GAPDH, CDK2, CDK4, Cyt C, and Oct1 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Prasad A., Khudaynazar N., Tantravahi R.V., Gillum A.M, & Hoffman B.S. (2016). ON 01910.Na (rigosertib) inhibits PI3K/Akt pathway and activates oxidative stress signals in head and neck cancer cell lines. Oncotarget, 7(48), 79388-79400.

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