DNA was extracted from individual guts following the protocol described in Baldo et al. (2015) (link). The region V3–V4 (~464 bp) of 16S rRNA was amplified with non-barcoded primers with TruthSeq adapters. Amplicons from individual specimens were barcoded and pooled (Supplementary Information ). The final library was sequenced on the Illumina MiSeq v.3 instrument (600 cycle cartridge, 300 bp paired end, San Diego, CA, USA) with 10% PhiX, at the Center for Genomic Regulation in Barcelona (Spain).
Miseq v 3 instrument
Manufactured by Illumina
Sourced in United States
The MiSeq v.3 instrument is a next-generation sequencing platform designed for small-scale genomic projects and targeted applications. It provides a compact, benchtop solution for DNA and RNA sequencing.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using miseq v 3 instrument
1
16S rRNA Gut Microbiome Profiling
2
RNA Detection in Extracellular Vesicles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For detection of RNA species in ELV preparations, small RNAs were preferentially isolated from vesicle-containing pellets using the miRCURY RNA Isolation Kit (Exiqon, Vedbaek, Denmark) and RNA samples were examined with an Agilent 2100 Bioanalyzer using the RNA 6000 Nano Kit. For small RNA sequencing (RNA-Seq), total RNA was isolated from ELVs released by ∼5,000 L3s over a 24 hr incubation period using the Total RNA and Protein Isolation Kit (Invitrogen, Carlsbad, CA). In parallel, total RNA was isolated from whole worm tissue using a TRIzol (Invitrogen) protocol, where a 6 hr precipitation step was carried out at -80°C to improve small RNA recovery. RNA NGS libraries were constructed using modified Illumina adapter methods using SBI’s XRNA Sample Preparation Kit (System Biosciences, Mountain View, CA) and indexed with separate bar codes for multiplex sequencing on an Illumina MiSeq v3 instrument using a 2 × 75 bp paired end run setting.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!