Cd27 fitc m t271

Manufactured by BD

Sourced in United States

CD27 FITC (M-T271) is a fluorescently labeled monoclonal antibody that binds to the CD27 cell surface antigen. CD27 is a member of the tumor necrosis factor receptor superfamily and is expressed on various immune cell types.

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Lab products found in correlation

Cd19 pe lt19, by Miltenyi Biotec (2 mentions) Ifn α, by Merck Group (1 mentions) Cd138 allophycocyanin b b4, by Miltenyi Biotec (1 mentions) Cd38 af700, by BioLegend (1 mentions) Cd20 efluor v450 2h7, by Thermo Fisher Scientific (1 mentions) Cd27 af647 lt27, by Bio-Rad (1 mentions) Cd19 percp cy5.5 sj225c1, by BD (1 mentions) Cd24 fitc ml5, by BD (1 mentions) Cd95 bv421, by BioLegend (1 mentions) Cd27 bv605, by BioLegend (1 mentions) Cd3viogreen bw264 56, by Miltenyi Biotec (1 mentions) Unconjugatedgoat anti irf4 m 17, by Santa Cruz Biotechnology (1 mentions) Donkey anti goat igg af488, by Thermo Fisher Scientific (1 mentions) Goat anti human f ab 2 fragments anti igm and igg, by Jackson ImmunoResearch (1 mentions) Human il 2, by Thermo Fisher Scientific (1 mentions) Hybridomax hybridoma growth supplement, by Gentaur (1 mentions) Mem amino acids solution, by Merck Group (1 mentions) Ki 67 fitc b56, by BD (1 mentions) Il 21, by Thermo Fisher Scientific (1 mentions) Lipid mixture 1, by Merck Group (1 mentions)

2 protocols using cd27 fitc m t271

Multiparametric Flow Cytometry of B Cells

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Antibodies used were CD19 PE (LT19; Miltenyi), CD138 allophycocyanin
(B-B4; Miltenyi Biotec), CD38 PE-Cy7 (HB7; BD Biosciences), CD38 AF700 (HIT2;
Biolegend), CD20 efluor V450 (2H7; eBioscience); CD27 AF647 (LT27; AbD Serotec),
CD27 FITC (M-T271), CD19 PerCP-Cy5.5 (SJ225C1; BD Biosciences), CD19 PE-Cy7
(SJ225C1; BD Biosciences), CD24 FITC(ML5; BD Biosciences), CD84 PE (CD84.1.21;
Biolegend), CD38 PerCP-Cy5.5 (HIT2; BD Biosciences), CD95 BV421 (DX2;
Biolegend), CD20 APC-H7(L27; BD Biosciences), CD27 BV605 (O323; Biolegend), CD3
VioGreen (BW264/56; Miltenyi), Ki67 FITC (B56; BD Biosciences), unconjugated
goat anti-IRF4 (M-17; Santa Cruz) and donkey anti-goat IgG AF488 (Polyclonal;
Invitrogen). Controls were isotype-matched mouse mAbs. Annexin V FITC was from
eBioscience and 7-AAD from BD Biosciences.
Reagents included human IL-2 (Roche); IL-6 (Peprotech) and IFN-α
(Sigma); IL-21 (PeproTech); goat anti-human F(ab′)₂ fragments
(anti-IgM and -IgG; Jackson Immunoresearch); HybridoMax hybridoma growth
supplement (Gentaur); Lipid Mixture 1, chemically defined (200X) and MEM Amino
Acids Solution (50X; Sigma).

Arumugakani G., Stephenson S.J., Newton D.J., Rawstron A., Emery P., Doody G.M., McGonagle D, & Tooze R.M. (2017). Early Emergence of CD19-Negative Human Antibody-Secreting Cells at the Plasmablast to Plasma Cell Transition. The Journal of Immunology Author Choice, 198(12), 4618-4628.

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Multiparameter Flow Cytometry for Plasma Cell Phenotyping

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Flow cytometry was performed with 6-color direct immunofluorescence detection using a Cytoflex LX (Beckman Coulter, Brea, CA, USA). Staining was performed with the following antibodies: CD19 PE (LT19, Miltenyi 130113169), CD138 APC (44F9, Miltenyi 130117395), CD20 e450 (2H7, Thermo Fischer 48020942) (Thermo Fischer, Waltham, MA, USA), CD27 FITC (M-T271, BD 55540), and CD38 PECy7 (HB7, BD 335825). Isotype-matched antibodies were used as controls (IgG1—PE Miltenyi 130113200; IgG1—APC Miltenyi 130113196; IgG2b—eFlour450 Thermo Fisher 48473282; IgG1—FITC BD 348808; IgG1—PECy7 Miltenyi 130113196,). The zombie UV fixable viability kit (Biolegend 423108, San Diego, CA, USA) was used to exclude dead cells. FlowJo version 10 (BD Biosciences, Franklin Lakes, NJ, USA) and Prism 9 (GraphPad, San Diego, CA, USA) with Spearman correlation statistical analysis were applied for analysis.

Care M.A., Stephenson S., Owen R., Doody G.M, & Tooze R.M. (2023). Spontaneous EBV-Reactivation during B Cell Differentiation as a Model for Polymorphic EBV-Driven Lymphoproliferation. Cancers, 15(12), 3083.

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