Cd11b human and mouse macs microbeads

RPMI 1640, Lipopolysaccharide (LPS), Penicillin Streptomycin solution, NaNO₂, Sphingosine 1 Phosphate and anti-Actin antibody were purchased from Sigma (Taufkirchen, Germany). Sulphanilamide and N-(naphthyl) ethylene-diaminedihydrochloride were purchased from E-Merck (Darmstadt, Germany). CD11b+ human and Mouse MACS Microbeads and MS Columns were purchased from MiltenyiBiotec (Auburn, CA).Recombinant mouse IFN cytokines, rabbit polyclonal anti-mouseNOS2,eNOS, and pAKT and Caveolin-1 antibodies were purchased from Cell Signaling (Frankfurt, Germany). VEGFR2 and anti-HIF-1antibodies were purchased from Novus Biological (Wiesbaden, Germany). VEGFpeptide was purchased from Pepro-Tech GmBH (Hamburg, Germany). Goat anti-rabbit and mouse-HRP conjugated secondary antibodies were purchased from Sigma (Taufkirchen, Germany)and Santa Cruz (Heidelberg Germany), respectively. Mouse HIF-1 specific siRNAwas purchased from Invitrogen (Schwerte, Germany). Control siRNA was purchased from Qiagen (Hilden, Germany). Goat anti-rabbit Alexa flour 488, goat anti-rat -Alexa flour 569, anti-rat-Cy3 and goat anti-mouse-Cy3 conjugated antibodies were purchased from Di-nova (Koenigswinter, Germany).