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Srbcs

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SRBCs, or Sheep Red Blood Cells, are a type of laboratory reagent used in various biological and immunological applications. They are derived from the blood of sheep and are commonly used as a model system for studying cellular processes, such as cell-mediated immunity, complement activation, and cellular signaling. SRBCs provide a standardized and consistent source of red blood cells for experimental purposes.

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Lab products found in correlation

Np ficoll, by Biosearch Technologies (2 mentions) Np cgg, by Biosearch Technologies (1 mentions) Np lps, by Biosearch Technologies (1 mentions) Np klh, by Biosearch Technologies (1 mentions) Np4 bsa or np 32 bsa coated plates, by Vector Laboratories (1 mentions)

7 protocols using srbcs

1

LPS-Induced Immune Responses in Mice

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For the LPS challenge experiments, mice were injected with 50 μg of LPS intraperitoneally. After 4 h, the splenocytes were obtained and analyzed for DC maturation and T-cell activation. For the adoptive transfer experiments, 1 × 106 of CFSE-labeled OT-II CD4+ T-cells were injected intravenously into recipient mice. After 1 day, the recipient mice were immunized with 15 μg OVA and 15 μg LPS mixed in PBS by intraperitoneal injection. Splenocytes were isolated from the mice 3 days later and analyzed for OT-II cell activation and proliferation. For sheep RBC (SRBC) immunization, 100% packed SRBCs (Innovative Research, Novi, MI, USA) were washed and resuspended in PBS, and 2 × 108 SRBCs were injected intraperitoneally into mice. A week later, the splenocytes were isolated and analyzed for follicular helper T-cells.
Lee H.J., Park J.S., Yoo H.J., Lee H.M., Lee B.C, & Kim J.H. (2020). The Selenoprotein MsrB1 Instructs Dendritic Cells to Induce T-Helper 1 Immune Responses. Antioxidants, 9(10), 1021.
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2

Immunomodulatory Effects of Phyllanthus amarus

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Animals were put into six groups, with six rats in each group. The animals in all groups were immunized with sRBCs obtained from Innovative Research, Inc. The antigen was prepared by washing the sRBCs three times with cold PBS. The washed sRBCs were then resuspended in PBS prior to immunization. sRBC immunization was performed by injecting 5.0×108 sRBCs/mL intraperitoneally (IP) in 200 μL, 2 weeks after each group was treated with either P. amarus (100, 200, or 400 mg/kg PO, daily), cyclophosphamide (100 mg/kg, PO, daily), or vehicle.
Ilangkovan M., Jantan I., Mesaik M.A, & Bukhari S.N. (2015). Immunosuppressive effects of the standardized extract of Phyllanthus amarus on cellular immune responses in Wistar-Kyoto rats. Drug Design, Development and Therapy, 9, 4917-4930.
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3

Murine Immunization and Memory Response

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Mice were intraperitoneally injected with 50 µg NP-CGG (in alum; range 30–39; no. N5055D; LGC Biosearch Technologies), NP-Ficoll (no. F-1420; LGC Biosearch Technologies), NP-LPS (no. N5065; LGC Biosearch Technologies), and 100 million SRBCs (no. IC10-0210; Innovative Research) on day 0, followed by a boost on day 10 and analysis of immune response on day 14. For the memory experiment, after the initial scheme as the primary response immunization, the mice were again challenged with 50 µg NP-CGG in PBS at 8 wk and analyzed 1 wk after the rechallenge.
Vaidyanathan B., Chaudhry A., Yewdell W.T., Angeletti D., Yen W.F., Wheatley A.K., Bradfield C.A., McDermott A.B., Yewdell J.W., Rudensky A.Y, & Chaudhuri J. (2017). The aryl hydrocarbon receptor controls cell-fate decisions in B cells. The Journal of Experimental Medicine, 214(1), 197-208.
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4

Investigating T-dependent and T-independent B Cell Responses

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To study T-independent responses, mice were prebled, immunized with 100ug/mL NP-Ficoll (Biosearch Technologies) in PBS, and bled 7 and 14 days later. For T-dependent responses, mice were prebled, immunized with 500 ug/mL NP-KLH (Biosearch Technologies) in alum (ThermoFisher Scientific), and bled 7, 14, and 28 days later. After the 28 day bleed, mice were boosted with 1mg/mL NP-KLH (Biosearch Technologies) in PBS and bled one week later. For germinal center studies, mice were immunized with 2.5×108 sheep red blood cells (SRBCs) (Innovative Research) or PBS and germinal centers measured by flow cytometry 5 days later (see above).
Ottens K., Schneider J., Kane L.P, & Satterthwaite A.B. (2020). PIK3IP1 promotes extrafollicular class switching in T-dependent immune responses. Journal of immunology (Baltimore, Md. : 1950), 205(8), 2100-2108.
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5

Rapamycin Modulates Germinal Center

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Mice were immunized i.p. with 100 μL of a final count of 5 × 108 washed SRBCs (Innovative Research) and sacrificed 8 days after immunization. Mice were immunized with 100 μL of NP(18)-LPS (Biosearch Technologies) mixed at a 1:1 ratio with Imject alum (Pierce) at a final concentration of 0.05 mg/mL and sacrificed on day 10. Mice were injected i.p. with 100 μL PBS vehicle, 100 μL at 1 mg/kg, 0.3 mg/kg or 0.075 mg/kg Rapamycin. Blood was collected for serum and spleen was harvested for measuring germinal center and T follicular helper cells.
Chiu H., Jackson L.V., Oh K.I., Mai A., Ronai Z.A., Ruggero D, & Fruman D.A. (2018). The mTORC1/4E-BP/eIF4E Axis Promotes Antibody Class Switching in B Lymphocytes. Journal of immunology (Baltimore, Md. : 1950), 202(2), 579-590.
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6

Immunization and Influenza Virus Infection in Mice

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For SRBC immunization, SRBCs were obtained from Innovative Research and washed with phosphate-buffered saline (PBS) until the supernatant was clear. Mice were immunized intraperitoneally with 1 × 109 SRBC to induce T-dependent GC response. For influenza virus infection, mice were challenged intranasally with 50 μl of either a sublethal dose [20 plaque-forming units (PFU)] of influenza virus AH3N2 ×31 (PR8 backbone: HA and NA from A/Aichi/2/68) (44 (link)) or 100 PFU of influenza virus AH1N1 (A/Puerto Rico/8/34, PR8) at egg-grown preparation diluted in PBS under mild ketamine/xylazine anesthesia. Body weight loss was measured on a daily basis as a readout for morbidity. All experiments were approved and performed according to the guidelines of the Icahn School of Medicine at Mount Sinai IACUC (IACUC-2016-0074). The methods used were carried out in accordance with the approved guidelines. Mice were euthanized when they had reached the ethical end point of 20% body weight loss.
Horiuchi S., Wu H., Liu W.C., Schmitt N., Provot J., Liu Y., Bentebibel S.E., Albrecht R.A., Schotsaert M., Forst C.V., Zhang B, & Ueno H. (2021). Tox2 is required for the maintenance of GC TFH cells and the generation of memory TFH cells. Science Advances, 7(41), eabj1249.
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7

LCMV Infection and TFH Induction in Mice

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Eight to 12 week-old gender-matched 6Y/6Y and 6F/6F mice were infected intravenously with 2 × 106 PFU of LCMV Armstrong clone 53b. Stocks were prepared by a single passage on BHK-21 cells, and viral titres were determined by plaque formation on Vero cells. For in vivo TFH induction, mice were injected intraperitoneally with SRBCs (5 × 108; Innovative Research) or NP-CGG, NP-KLH (100 μg; Vector Labs). IgM and IgG enzyme-linked immunosorbent assays (BD Biosciences) were performed with NP4-BSA- or NP32-BSA-coated plates (Vector Labs).
Hwang S., Palin A.C., Li L., Song K.D., Lee J., Herz J., Tubo N., Chu H., Pepper M., Lesourne R., Zvezdova E., Pinkhasov J., Jenkins M.K., McGavern D, & Love P.E. (2015). TCR ITAM multiplicity is required for the generation of follicular helper T-cells. Nature Communications, 6, 6982.
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