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6 aminopenicillanic acid

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(+)-6-Aminopenicillanic acid is a chemical compound that serves as the core structure of penicillin antibiotics. It is a key intermediate in the production of various penicillin derivatives.

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Lab products found in correlation

Phenylacetic acid, by Merck Group (2 mentions) Penicillin g sodium salt, by Merck Group (2 mentions) P nitroaniline, by Merck Group (2 mentions) Dextro benzylpenicilloic acid hydrate, by Merck Group (2 mentions) P nitroacetanilide, by Merck Group (2 mentions) N phenylacetyl p aminobenzoate, by Merck Group (2 mentions) L glutamate 1 p nitroaniline, by Merck Group (2 mentions) L glutamate γ p nitroaniline, by Merck Group (2 mentions) Nα benzoyl dl arginine p nitroaniline, by Merck Group (2 mentions) M nitrophenol, by Thermo Fisher Scientific (2 mentions) P nitrophenol, by MP Biomedicals (2 mentions) Acetonitrile, by Merck Group (1 mentions) Ammonium acetate, by Merck Group (1 mentions) Acetic acid, by Merck Group (1 mentions) Arium water purification system, by Sartorius (1 mentions)

3 protocols using 6 aminopenicillanic acid

1

Analytical HPLC Characterization of Compounds

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All standard compounds used (glucosamine, erythritol, arabitol and mannitol; purity ≥98%) as well as chemicals (acetic acid, ammonium acetate, 6-aminopenicillanic acid, etc.) and solvents (acetonitrile) were purchased from Sigma (St. Louis, MO, USA) or Merck (Darmstadt, Germany). HPLC-grade water for analysis was obtained from an Arium water purification system (Sartorius, Göttingen, Germany).
Artmann D.J., Amrain W., Murauer A., Ganzera M., Vrabl P., Schinagl C.W, & Burgstaller W. (2019). Critical evaluation of a putative glucosamine excretion by Aspergillus niger CBS120.49 and Penicillium ochrochloron CBS123.824 under citric acid producing conditions. Scientific Reports, 9, 7496.
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2

Enzymatic Assays and Growth Studies

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For growth studies and enzymatic assays high purity penicillin G sodium salt (Sigma, P3032), (+)-6-aminopenicillanic acid (Sigma, A70909), phenylacetic acid (Sigma, W287806), and other antibiotics, were purchased. Dextro-(−)-benzylpenicilloic acid hydrate (Sigma, S341967) was purchased from the Sigma-Aldrich collection of rare and unique chemicals. p-Nitroacetanilide (Sigma, 130648), p-nitroaniline (Sigma, 185310), 6-nitro-3-(phenylacetamido)benzoic acid (aka NIPAB, Pfaltz and Bauer, N10625), N-phenylacetyl-p-aminobenzoate (Sigma, P8529), L-glutamate 1-(p-nitroaniline) (Sigma, 49622), L-glutamate γ-(p-nitroaniline) (Sigma, G1135) and Nα-benzoyl-DL-arginine p-nitroaniline (Sigma, B4875) were purchased as enzyme substrates and standards. For the kinetics assays p-nitrophenol (MP Biomedicals, 102461) and m-nitrophenol (Acros organics, 172300100) were purchased as indicators for monitoring of enzyme reaction kinetics in pH-shift assays. All other compounds and buffers used were of standard molecular biology grade.
Crofts T.S., Wang B., Spivak A., Gianoulis T.A., Forsberg K.J., Gibson M.K., Johnsky L.A., Broomall S.M., Rosenzweig C.N., Skowronski E.W., Gibbons H.S., Sommer M.O, & Dantas G. (2018). Shared strategies for β-lactam catabolism in the soil microbiome. Nature chemical biology, 14(6), 556-564.
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3

Enzymatic Assays and Growth Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
For growth studies and enzymatic assays high purity penicillin G sodium salt (Sigma, P3032), (+)-6-aminopenicillanic acid (Sigma, A70909), phenylacetic acid (Sigma, W287806), and other antibiotics, were purchased. Dextro-(−)-benzylpenicilloic acid hydrate (Sigma, S341967) was purchased from the Sigma-Aldrich collection of rare and unique chemicals. p-Nitroacetanilide (Sigma, 130648), p-nitroaniline (Sigma, 185310), 6-nitro-3-(phenylacetamido)benzoic acid (aka NIPAB, Pfaltz and Bauer, N10625), N-phenylacetyl-p-aminobenzoate (Sigma, P8529), L-glutamate 1-(p-nitroaniline) (Sigma, 49622), L-glutamate γ-(p-nitroaniline) (Sigma, G1135) and Nα-benzoyl-DL-arginine p-nitroaniline (Sigma, B4875) were purchased as enzyme substrates and standards. For the kinetics assays p-nitrophenol (MP Biomedicals, 102461) and m-nitrophenol (Acros organics, 172300100) were purchased as indicators for monitoring of enzyme reaction kinetics in pH-shift assays. All other compounds and buffers used were of standard molecular biology grade.
Crofts T.S., Wang B., Spivak A., Gianoulis T.A., Forsberg K.J., Gibson M.K., Johnsky L.A., Broomall S.M., Rosenzweig C.N., Skowronski E.W., Gibbons H.S., Sommer M.O, & Dantas G. (2018). Shared strategies for β-lactam catabolism in the soil microbiome. Nature chemical biology, 14(6), 556-564.
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