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Vevo 3100 ultrasound system

Manufactured by Fujifilm
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The Vevo 3100 ultrasound system is a medical imaging device designed for high-resolution, real-time visualization and analysis of small animal anatomy. It utilizes high-frequency sound waves to generate detailed images of internal structures.

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Lab products found in correlation

Mx550d transducer, by Fujifilm (2 mentions) Vevo lab 3, by Fujifilm (1 mentions) Ms 550 transducer, by Fujifilm (1 mentions) Vevo software, by Fujifilm (1 mentions)

16 protocols using vevo 3100 ultrasound system

1

Cardiac Function Evaluation in Pdgfrα-CreER;ROSA-DTA Mice

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8-week-old Pdgfrα-CreERT2;ROSA-DTA mice had intraperitoneal tamoxifen injection as described in Figure S3H. Echocardiography was performed using a Vevo 3100 ultrasound system (Fujifilm VisualSonics), equipped with a MX550S transducer. B-mode and M-mode data were acquired following the manufacturer’s guidelines.
Mohr M.E., Li S., Trouten A.M., Stairley R.A., Roddy P.L., Liu C., Zhang M., Sucov H.M, & Tao G. (2023). Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury. bioRxiv.
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2

In vivo evaluation of KRAS-targeted nanotherapeutics

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Mice were randomly assigned to five groups (n = 5) and IP injected with PBS, PCX/(anti-miR-NC+siNC), PCX/(anti-miR-210+siNC), PCX/(anti-miR-NC+siKRASG12D), and PCX/(anti-miR-210+siKRASG12D), respectively. PCX/(anti-miRNA +siRNA) nanoparticles (5 mg/kg PCX) were injected at days 14, 16, 18, 20, 22, 24, and 26 post tumor-implantation. Mouse body weight was recorded during treatment. Tumor growth was monitored using a Vevo 3100 ultrasound system (Fujifilm, VisualSonics, Toronto, ON) with an MX550D transducer (40 MHz center frequency, 40 μM axial resolution) in B-mode. Briefly, mice were anesthetized using isoflurane and placed on a stationary, heated platform equipped with a motorized transducer adapter to acquire 3D images. Vevo Lab software was used to reconstruct tumor shapes and assess tumor volumes. On days 28, blood was harvested from retro-orbital venous plexus of the mouse for whole blood analysis and serum biochemistry test. Mice were sacrificed, and primary tumor weight and metastatic sites were evaluated in each experimental group. Total RNA in the tumor was isolated using mirVana miRNA Isolation Kit. Protein was also harvested from tumor tissues by RIPA Lysis buffer. Tumors and major organs were fixed in 4% paraformaldehyde and sectioned for histochemical analysis. Survival analysis was performed on mice with the same treatment method but using nine mice per group.
Xie Y., Hang Y., Wang Y., Sleightholm R., Prajapati D.R., Bader J., Yu A., Tang W., Jaramillo L., Li J., Singh R.K, & Oupický D. (2020). Stromal Modulation and Treatment of Metastatic Pancreatic Cancer with Local Intraperitoneal Triple miRNA/siRNA Nanotherapy. ACS nano, 14(1), 255-271.
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3

Echocardiographic Assessment of Murine Cardiac Function

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All mice underwent a transthoracic echocardiography protocol using VEVO 3100 Ultrasound system (FUJIFILM VisualSonics, Inc., Toronto, ON, Canada). A 2-dimensional (2D) parasternal short- and long-axis brightness (B-mode) of the mid-left ventricle (LV) was obtained at the level of the papillary muscles. Additionally, a 2D targeted motion-mode (M-mode) tracing was recorded to measure end diastolic and end systolic wall thicknesses and internal chamber dimensions. LV ejection fraction (%EF) and fractional shortening (%FS) were calculated as an index of LV systolic performance. Doppler-based flow velocity measurements were also taken to determine mitral valve E-wave/A-wave (MV E/A) ratios for diastolic LV function. MV E/A ratio marks changes in the peak velocity of blood flow from the LV during early diastole (E-wave) to the peak velocity of blood flow into the LV by atrial contraction during late diastole (A-wave). The MV E/A ratio represents LV filling velocity between systolic periods. Mice were maintained at ~1% isoflurane anesthesia during the echocardiography procedure. A minimum of 5 cardiac cycles were used to generate the data obtained. Heart measurements were conducted as described previously [58 (link),59 (link)] after either 2- or 21-days of wash-out from Dox.
Qrareya A.N., Wise N.S., Hodges E.R., Mahdi F., Stewart JA J.r, & Paris J.J. (2022). HIV-1 Tat Upregulates the Receptor for Advanced Glycation End Products and Superoxide Dismutase-2 in the Heart of Transgenic Mice. Viruses, 14(10), 2191.
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4

Quantifying Popliteal Lymph Nodes via 3D Ultrasound

Cited 2 times
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Mice were anesthetized with 1–3% isoflurane and imaged using a Vevo 3100 ultrasound system with the MX700 probe (Fujifilm, Toronto, ON, Canada). Depilatory cream was used to remove the fur on the posterior aspect of both hindlimbs, and mice were placed in the prone position on a temperature-controlled platform set at 37 °C. Hindpaws were secured with adhesive tape to identify the PLNs posterior to the knee, and B-mode images were collected as stacks across the PLN volume. Image stacks (.tif) were then imported into Amira software as luminance image types (v2020.2; ThermoFisher Scientific, FEI, Hillsboro, OR, USA; https://www.thermofisher.com/in/en/home/electron-microscopy/products/software-em-3d-vis/amira-software.html). The lasso tool with auto-trace was used to segment multiple sections of the PLN, which were connected using the interpolate function for volume quantification, as previously described15 (link),29 (link). Ultrasound was performed biweekly starting at 2-months-old in the TNF-Tg cohorts, and continued through the 6-week treatment period for all WT and TNF-Tg treatment groups.
Kenney H.M., Peng Y., Bell R.D., Wood R.W., Xing L., Ritchlin C.T, & Schwarz E.M. (2022). Persistent popliteal lymphatic muscle cell coverage defects despite amelioration of arthritis and recovery of popliteal lymphatic vessel function in TNF-Tg mice following anti-TNF therapy. Scientific Reports, 12, 12751.
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5

Ultrasound Evaluation of Liver Nodules in Mice

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Mice were weighed weekly and regular husbandry checks were performed for signs of anemia, jaundice, weight loss, and dermatitis. Mice were euthanized if they appeared sick per institutional guidelines. Ultrasound evaluation was performed at 46 weeks of age using the VisualSonics Vevo 3100 ultrasound system (Fujifilm, VisualSonics, Toronto, ON) with the MX550D transducer (40 MHz center frequency, 40 µM axial resolution). In brief, mice were anesthetized using 1-3% isoflurane, placed on a heated monitoring table, and had abdominal hair removed. B-mode images and 3D volumes were acquired in the abdominal area where liver nodules were investigated. Vevo Lab software was used to reconstruct nodule shapes and to measure nodule volumes. Nodules that measured 1mm × 1mm were considered false positives.
Vlock E.M., Karanjit S., Talmon G, & Farazi P.A. (2020). Reduction of Polyunsaturated Fatty Acids with Tumor Progression in a Lean Non-Alcoholic Steatohepatitis-Associated Hepatocellular Carcinoma Mouse Model. Journal of Cancer, 11(19), 5536-5546.
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6

Echocardiographic Evaluation of Cardiac Function

Cited 1 time
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The cardiac function of the mice was measured using echocardiography as described previously (23 (link)). Serial Doppler echocardiography was performed using the Vevo® 3100 ultrasound system with a 40-MHz high-frequency ultrasonic probe (Fujifilm VisualSonics, Toronto, ON, Canada). Left ventricular end-diastolic diameter (LVEDD), left ventricular end-systolic diameter (LVESD), left ventricular end-diastolic volume (LVEDV), and left ventricular end-systolic volume (LVESV) were measured. Fractional shortening (FS) was calculated as (LVEDD − LVESD)/LVEDD×100%, and left ventricular ejection fraction (LVEF) was calculated as (LVEDV − LVESV)/LVEDV ×100%.
Zhao Q., Liu F., Zhao Q., Zhang J., Luo J., Li X, & Yang Y. (2021). Constitutive activation of ERK1/2 signaling protects against myocardial ischemia via inhibition of mitochondrial fragmentation in the aging heart. Annals of Translational Medicine, 9(6), 479.
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7

Measuring Abdominal Aorta Diameter by Ultrasound

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SMAs were scanned using a Vevo 3100 ultrasound system with an MS550 transducer (FUJIFILM VisualSonics Inc.). Mice were placed on a heated platform (37°C) to avoid hypothermia and anesthetized with isoflurane (1–2% vol/vol) to adjust the heart rate between 400 and 550 beats/minute. Color Doppler was used to confirm the pulsatile flow of the abdominal aorta. Then the probe was moved from the diaphragm caudally to visualize the SMA. A cine loop of the SMA was captured to define the maximum dilation of the SMA. Maximal luminal diameters were measured on the captured images using Vevo LAB 3.1.1 software (FUJIFILM VisualSonics Inc.).
Zhang J.M., Au D.T., Sawada H., Franklin M.K., Moorleghen J.J., Howatt D.A., Wang P., Aicher B.O., Hampton B., Migliorini M., Ni F., Mullick A.E., Wani M.M., Ucuzian A.A., Lu H.S., Muratoglu S.C., Daugherty A, & Strickland D.K. (2023). LRP1 protects against excessive superior mesenteric artery remodeling by modulating angiotensin II–mediated signaling. JCI Insight, 8(2), e164751.
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8

Tamoxifen-Induced Pdgfrα-CreER Mice Cardiac Analysis

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8-week-old Pdgfrα-CreERT2;ROSA-DTA mice had intraperitoneal tamoxifen injection as described in Figure S3H. Echocardiography was performed using a Vevo 3100 ultrasound system (Fujifilm VisualSonics), equipped with a MX550D transducer. B-mode and M-mode data were acquired following the manufacturer’s guidelines.
Mohr M.E., Li S., Trouten A.M., Stairley R.A., Roddy P.L., Liu C., Zhang M., Sucov H.M, & Tao G. (2024). Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury. iScience, 27(3), 109219.
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9

Echocardiographic Assessment of Cardiac Function in Mice

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In order to detect the heart function, all the mice were anesthetized (2% isoflurane) and trans-thoracic M-mode echocardiography to measure systolic function by a VisualSonics Vevo 3100 ultrasound system (FUJIFILM VisualSonics Inc., Toronto, Canada) [15] (link). All hair was removed before the echo examination. Parasternal longaxis and short-axis views were acquired. The left ventricle (LV) ejection fraction (EF) and fractional shortening (FS) were calculated.
Li Z., Wang H., Zoungrana L.I., James A., Slotabec L., Didik S., Fatmi M.K., Krause-Hauch M., Lesnefsky E.J, & Li J. (2023). Administration of metformin rescues age-related vulnerability to ischemic insults through mitochondrial energy metabolism. Biochemical and biophysical research communications, 659.
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10

Echocardiographic Assessment of Cardiac Function in Mice

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Transthoracic echocardiography was performed after MI using a Vevo 3100 Ultrasound system (VisualSonics, Toronto, Canada). Mice were anesthetized with 1.5% isoflurane, and the heart rate of mice was preserved at approximately 550 bpm. Two-dimensional parasternal long-axis views of the left ventricle were obtained for guided M-mode measurements of the Dd and Ds. EF and FS were measured based on M-mode images.
Sun J.Y., Du L.J., Shi X.R., Zhang Y.Y., Liu Y., Wang Y.L., Chen B.Y., Liu T., Zhu H., Liu Y., Ruan C.C., Gan Z., Ying H., Yin Z., Gao P.J., Yan X., Li R.G, & Duan S.Z. (2023). An IL-6/STAT3/MR/FGF21 axis mediates heart-liver cross-talk after myocardial infarction. Science Advances, 9(14), eade4110.
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