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2 protocols using potassium phosphate monobasic

1

Nanoparticle Formulation Development

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CS (low molecular weight (MW), ≥75% deacetylated), GG (MW 967,000 g/mol), TPP (technical grade 85%, MW 367.86 g/mole), GLA (grade II 25% in water, MW 100.12 g/mol), CG, dipotassium hydrogen phosphate and sodium chloride were purchased from Sigma Aldrich, Steinheim, Germany. CX was kindly donated by Getz Pharma Private Limited, Karachi, Pakistan. Potassium phosphate monobasic was purchased from Duksan, Ansan, South Korea. Distilled water of an in-house facility (Department of Pharmaceutics, Faculty of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan) was used throughout the study. All other materials used were of analytical grade.
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2

Quantitative Analysis of Antidiabetic Compounds

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Voglibose (>98.0%) was purchased from Tokyo Chemical Industries (Tokyo, Japan). Erythromycin, oxytetracycline hydrochloride, cefadroxil, 4-nitrophenyl β-D-glucopyranoside, 4-nitrophenyl β-D-glucuronide, 4-nitrophenyl sulfate, soluble starch, ammonium acetate, streptozotocin, and telmisartan, the internal standard (IS), were purchased from Sigma-Aldrich (St. Louis, MO, USA). High-performance liquid chromatography (HPLC)-grade acetonitrile was purchased from J. T. Bakers (Central Valley, PA, USA). An anaerobe gas generating system was purchased from Becton, Dickinson and Company (Sparks, MD, USA). A blood glucose monitoring system was purchased from Bayer Contour TS (Mishawaka, IN, USA). Potassium phosphate monobasic and potassium phosphate dibasic were purchased from Duksan (Seoul, Korea). All other chemicals were of analytical grades and used as received.
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