Anti rabbit ig

Manufactured by Jackson ImmunoResearch

Sourced in United States

Anti-rabbit Ig is a secondary antibody product designed for the detection and quantification of rabbit primary antibodies in various immunoassays and research applications. The product binds specifically to the Fc region of rabbit immunoglobulins, allowing for the visualization or capture of rabbit antibodies in experimental procedures.

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Lab products found in correlation

Lamp1, by Merck Group (2 mentions) Tsg101, by Abcam (1 mentions) Fugene hd, by Promega (1 mentions) Calnexin, by Enzo Life Sciences (1 mentions) Anti goat ig, by Jackson ImmunoResearch (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Rabbit anti-rat Ig (3 citations) Goat anti-rabbit Ig-HRP (3 citations) Donkey anti-rabbit Ig (2 citations) HRP-conjugated donkey anti-rabbit and anti-mouse immunoglobulin (Ig) G secondary antibodies (2 citations) HRP-coupled goat anti-rabbit Ig (2 citations) Goat-anti rabbit mouse Ig conjugated to Cy3 (2 citations) Horseradish peroxidase-conjugated donkey anti-rabbit Ig (2 citations)

3 protocols using anti rabbit ig

Antibodies for Protein Analysis

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The following antibodies were purchased: c-Kit (M-14), STAT3 (C-20), STAT5 (C-17), Erk2 (K-23), Jak2 (C-20), cathepsin D (H-75), CD63 (H-93), actin (I-19) and CD28 (H-193) from Santa Cruz Biotechnology; Kit[pTyr719], Akt, Akt[pSer473], STAT5[pTyr694] (D47E7), cleaved caspase-3 and Erk[pThr202/pTyr204] (E10) from Cell Signaling Technology; TGN46, EEA1, Rab11, Tsg101 and TfR from Abcam; calnexin and ubiquitin (FK2) from Enzo; GM130 (35) and AP2α (8) from BD Transduction Laboratories; p85 from Millipore and LAMP1 from Sigma-Aldrich. Anti-phosphotyrosine antibody (4G10) was kindly provided by Dr Toshinori Nakayama (Chiba University). Alexa-fluor 488 anti-Kit (AF488-anti-Kit; 2B8; Biolegend) was used for the experiments in Figs 2b and 5k. The list of antibodies with source and conditions of immunoblotting and immunofluorescence is shown in Supplementary Table 1. HRP-labelled anti-mouse Ig, anti-rabbit Ig and anti-goat Ig secondary antibodies were purchased from The Jackson Laboratory. AF488-anti-goat IgG, AF568-anti-rabbit IgG, AF647-anti-goat IgG and AF647-anti-mouse secondary antibodies were obtained from Molecular Probes.

Obata Y., Toyoshima S., Wakamatsu E., Suzuki S., Ogawa S., Esumi H, & Abe R. (2014). Oncogenic Kit signals on endolysosomes and endoplasmic reticulum are essential for neoplastic mast cell proliferation. Nature Communications, 5, 5715.

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Antibody and Cell Line Protocols

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Chemicals were from Sigma, unless otherwise indicated. Monoclonal and polyclonal anti-ERp44 antibodies (36C9 and JDA1 respectively), anti-myc (9E10) and anti-GM130 were previously described (Anelli et al., 2002 (link); Anelli et al., 2007 (link)). Polyclonal anti-PDI was a kind gift of I. Braakman (Utrecht, NL). Unconjugated goat anti-mouse Ig were from Southern Biotechnology Associates, Inc. (Birmingham, AL), while horse-radish peroxidase (HRP) bound goat anti-mouse Ig and anti-rabbit Ig were from Jackson ImmunoResearch Laboratories, Inc. Fluorescent goat anti-mouse and anti-rabbit IgG (H+L) conjugated to Alexa Fluor 700, 647, 546 and 488 were from Invitrogen Molecular Probes (Eugene, Oregon, USA).
HeLa and HepG2 cell lines were purchased from ATCC. Tissue culture, transfection, and silencing were performed as described previously (Anelli et al., 2007 (link)). HepG2 cells were transfected by FugeneHD (Promega Corporation, Madison, USA) following the manufacturer instructions. Primary endometrial cells were collected from healthy donors and cultured as described by Di Blasio at al. (1995) (link) (Di Blasio et al., 1995 (link)).

Sannino S., Anelli T., Cortini M., Masui S., Degano M., Fagioli C., Inaba K, & Sitia R. (2014). Progressive quality control of secretory proteins in the early secretory compartment by ERp44. Journal of cell science, 127(19), 4260-4269.

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Antibody Inventory for Protein Profiling

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The following antibodies were purchased: Kit (M-14), STAT5 (C-17), Erk2 (K-23) and cathepsin D (H-75) from Santa Cruz Biotechnology (Dallas, TX); Kit[pTyr719], Kit[pTyr703], Akt (40D4), Akt[pT308] (C31E5E), STAT5[pTyr694] (D47E7), Erk[pThr202/pTyr204] (E10), and cleaved caspase-3 from Cell Signaling Technology (Danvers, MA); p85, phospho-tyrosine (4G10), and Kit[pTyr568/570] from Millipore (Billerica, MA); LAMP1 from Sigma (St. Louis, MO); Kit[pTyr936] from Thermo Scientific Pierce (Rockford, IL) and calnexin from Enzo Life Sciences (Farmingdale, NY). HRP-labeled anti-mouse Ig, anti-rabbit Ig, and anti-goat Ig secondary antibodies were purchased from the Jackson Laboratory (Bar Harbor, MA). Alexa-Fluor-conjugated secondary antibodies were obtained from Molecular Probes (Eugene, OR).

Hara Y., Obata Y., Horikawa K., Tasaki Y., Suzuki K., Murata T., Shiina I, & Abe R. (2017). M-COPA suppresses endolysosomal Kit-Akt oncogenic signalling through inhibiting the secretory pathway in neoplastic mast cells. PLoS ONE, 12(4), e0175514.

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