Ez rna 2 isolation kit

Total RNA was extracted 3 days post infection using EZ-RNA II isolation kit (Biological Industries, Beit Haemek, Israel), according to the manufacturer’s protocol. Aliquots of total RNA were used directly for miRNAs quantification using qPCR or subjected to small RNA library construction.

Total RNA was extracted using the EZ-RNA II Isolation Kit reagent (Biological Industries Beit Haemek, Israel) according to manufacturer's instructions. Briefly, cells were lysed with guanidine thiocyanate detergent solution, followed by organic extraction and alcohol precipitation of the RNA. Quantification and the purity of total RNA were assessed by using the NanoDrop1000® spectrophotometer (Thermo Fisher Scientific, MA, USA).
RNA isolation from exosomes was performed by using Total Exosome RNA and Protein Isolation Kit (Invitrogen, CA, USA). Exosomes were lysed with denaturing solution and then precipitated by acid-phenol: chloroform solution followed by several washes and centrifugation steps. The supernatant was then placed on filter cartridge tubes for further purification using numerous washing solutions provided by the kit. RNA was eluted with PCR grade water.

RNA extraction from cells was performed using the EZ-RNA II Isolation Kit (Biological Industries Beit Haemek, Israel) according to the manufacturer's instructions. Extracted RNA was quantified by the NanoDrop spectrophotometer with ND-1000 software (Thermo scientific, MA, USA). The RNA extraction from exosomes was executed using the Total Exosome RNA and Protein Isolation Kit (Invitrogen, CA, USA) according to manufacturer's instructions.