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Evos fl

Manufactured by Leica
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The EVOS FL is a fluorescence microscope designed for live-cell imaging. It provides high-resolution, real-time visualization of fluorescently labeled samples. The EVOS FL is equipped with LED illumination, which offers consistent and long-lasting performance. It is a versatile tool for a wide range of applications in life science research.

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Lab products found in correlation

Alexa fluor 488 antibody, by Thermo Fisher Scientific (2 mentions) Lab tek 2 chamber slide, by Thermo Fisher Scientific (2 mentions) Anti phospho histone h2a x ser139 antibody, by Merck Group (2 mentions) X rad smart irradiator, by Precision X-Ray (2 mentions)

2 protocols using evos fl

1

Gamma-labeled Mesoporous Silica Nanoparticles for DNA Damage

Check if the same lab product or an alternative is used in the 5 most similar protocols
MDA-MB-231 cells (1 × 104 cells/well) were seeded on a Lab-Tek II Chamber Slide (cat# 154453, ThermoFisher Scientific). After 24 h, cold MSNs and 68Ga-MSNs (4.5 μg/mL) were prepared and used to label the cells. As a positive control, MDA-MB-231 cells were treated to 3 Gy and 6 Gy of X-ray radiation (225 kVp; X-RAD SmART irradiator, Precision X-Ray Inc). One hour after completion of these procedures, the cells were fixed in 4% formaldehyde and stained with anti-phospho-histone H2A.X (Ser139) antibody (1:100; cat# 05-636, Sigma-Aldrich) overnight at 4°C. Secondary staining was performed using anti-mouse Alexa Fluor 488 antibody (1:100; cat# A-21202, ThermoFisher Scientific). Staining was imaged by fluorescence microscopy (EVOS FL; and DMi8, Leica Microsystems). Mean fluorescence intensity per cell was quantified using ImageJ software.
Jung K.O., Kim T.J., Yu J.H., Rhee S., Zhao W., Ha B., Red-Horse K., Gambhir S.S, & Pratx G. (2020). Whole-body tracking of single cells via positron emission tomography. Nature biomedical engineering, 4(8), 835-844.
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2

Gamma-labeled Mesoporous Silica Nanoparticles for DNA Damage

Check if the same lab product or an alternative is used in the 5 most similar protocols
MDA-MB-231 cells (1 × 104 cells/well) were seeded on a Lab-Tek II Chamber Slide (cat# 154453, ThermoFisher Scientific). After 24 h, cold MSNs and 68Ga-MSNs (4.5 μg/mL) were prepared and used to label the cells. As a positive control, MDA-MB-231 cells were treated to 3 Gy and 6 Gy of X-ray radiation (225 kVp; X-RAD SmART irradiator, Precision X-Ray Inc). One hour after completion of these procedures, the cells were fixed in 4% formaldehyde and stained with anti-phospho-histone H2A.X (Ser139) antibody (1:100; cat# 05-636, Sigma-Aldrich) overnight at 4°C. Secondary staining was performed using anti-mouse Alexa Fluor 488 antibody (1:100; cat# A-21202, ThermoFisher Scientific). Staining was imaged by fluorescence microscopy (EVOS FL; and DMi8, Leica Microsystems). Mean fluorescence intensity per cell was quantified using ImageJ software.
Jung K.O., Kim T.J., Yu J.H., Rhee S., Zhao W., Ha B., Red-Horse K., Gambhir S.S, & Pratx G. (2020). Whole-body tracking of single cells via positron emission tomography. Nature biomedical engineering, 4(8), 835-844.
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