Leaf and twigs samples were freeze-dried, ground and passed through a 0.02 mm (mesh 70) sieve (Glenammer Sieves Ltd., UK). MIR spectra were acquired at ambient temperature (20 ± 2 °C) using a Nicolet iS50 FT-IR spectrometer Thermo Nicolet iS50 coupled with an ATR iD7 accessory (Thermo Fisher Scientific, Dublin, Ireland). The instrument uses a typical diamond crystal, ZnSe lens, and DTGS KBr detector. More specifically, after the sample holder was cleaned with alcohol and dust-free tissue, the samples were placed on the flat surface of the crystal while the slip clutch tower applied equal pressure. Thirty-two scans per sample were collected in the mid-infrared range from 550 to 4000 cm−1 in transmission mode at a spectral resolution of 4 cm−1. The spectra were corrected against air as background. Three different replicates of each sample were measured and averaged before data preprocessing. Each IR spectrum was normalized and the baseline corrected with OMNIC 7.3 software (Spectra-Tech Inc., Madison, WI, USA).
Id7 atr accessory
Manufactured by Thermo Fisher Scientific
Sourced in United States, Ireland, United Kingdom
The ID7 ATR accessory is a laboratory instrument designed to perform attenuated total reflectance (ATR) spectroscopy. The core function of the ID7 ATR accessory is to enable the collection of infrared spectra of solid, liquid, or gel samples with minimal sample preparation.
Automatically generated - may contain errors
Lab products found in correlation
Nicolet is5 ftir spectrometer, by Thermo Fisher Scientific (3 mentions)
Nicolet is5 spectrometer, by Thermo Fisher Scientific (2 mentions)
Nicolet is5, by Thermo Fisher Scientific (1 mentions)
Omnic software, by Thermo Fisher Scientific (1 mentions)
Infinite 200 pro plate reader, by Tecan (1 mentions)
400 mhz spectrometer, by Bruker (1 mentions)
Nicolet is50 ftir spectrometer, by Thermo Fisher Scientific (1 mentions)
10 protocols using id7 atr accessory
1
Leaf and Twig Sample Preparation for MIR Analysis
2
Spectroscopic Analysis of Food Samples
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Molecular spectroscopic experiments were conducted at the Institute for the Global Food Security, Queen's University Belfast. The analysis of samples by ATR-FT/IR was performed at ambient temperature using a Nicolet iS5 FT-IR spectrometer Thermo Nicolet iS5 and ATR iD7 accessory (Thermo Fisher Scientific, Dublin, Ireland) , with diamond crystal, ZnSe lens, and DTGS KBr detector. After the sample holder was cleaned with an alcohol swab, the samples were placed on the flat surface of the crystal while the slip clutch tower applied equal pressure. Thirty-two scans per sample were collected in the mid-infrared range from 550 to 4000 cm -1 in transmission mode at a spectral resolution of 4 cm -1 . The collected spectra were analysed against air as background. Three replicates of each sample were measured and averaged before data pre-processing. Before the measurements of peak heights and areas, each IR spectrum was normalised, and then its second derivative was generated and autosmoothed. The collection of the functional spectral bands associated with nutrient molecular structures, the corrections with the background spectrum and the data pre-processing were done with OMNIC 7.3 software (Spectra-Tech Inc., Madison, WI, USA).
3
ATR-FTIR Spectroscopy of Samples
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IR spectra were recorded using an iD7 ATR accessory and a Nicolet iS5 Spectrometer (ThermoFisher). 200 scans per sample.
4
Structural Analysis of Silk Fibroin Films
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Attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR) was used to analyze the structural changes of the SF in four kinds of films (the same as XRD). All infrared spectra were recorded in the range of 4000–550 cm−1 using the Nicolet iS5 spectrometer, equipped with an iD7 ATR accessory (Thermo Scientific, Waltham, MA, USA). Each spectrum was acquired by the accumulation of 32 scans with a resolution of 4 cm−1.
5
Polymer Identification in Microplastics
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Mid-infrared spectra were collected in an ATR mode in the wave-number range of 400–4000 cm−1 using a Nicolet iS5 (Thermo Scientific Inc., Waltham, MA, USA) FTIR spectrometer equipped with an iD7 ATR accessory (Thermo Scientific) and DTGS detector. For each sample, 32 scans were acquired at a resolution of 4 cm−1. The ATR-FTIR analysis was performed for all samples. The obtained spectra were compared with those of the reference samples representing the six most popular synthetic polymers (i.e., polyethylene (PE), polypropylene (PP), polyurethane (PUR), nylon-66 (Nyl-66), polystyrene (PS) and poly(ethylene terephthalate) (PET)). Additionally, to verify the hypothesis of the presence of particles of rubber in the MP originating from grated tyres, the ATR-FTIR spectra of two types of commercial rubber were added (the typical tyre materials are marked T3 and T7).
6
Functional Groups Analysis of Hydroxyapatite
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Fourier-transform infrared spectroscopy (FT-IR) was used to identify characteristic functional groups in the synthesized hydroxyapatite. Using a Thermo Scientific Nicolet iS5 FT-IR spectrometer (Thermo Scientific, Loughborough, UK) equipped with an iD7 ATR accessory operating under room conditions in the range of 4000 cm−1–400 cm−1 (32 scans at 4.0 cm−1 resolution), spectra were analyzed for the resulting ceramic powders as previously described [42 (link)].
7
FT-IR Characterization of Coatings
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In the present study, the individual functional groups in the obtained coatings were identified using Fourier transform infrared spectroscopy (FT-IR). The analysis was carried out using a Nicolet iS5 FT-IR spectrometer equipped with an iD7 ATR accessory (Thermo Scientific, Loughborough, UK) in the range from 4000 to 400 cm−1 (32 scans at 4.0 cm−1 resolution) under room conditions.
8
Structural Analysis of β-Glucan via FT-IR
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The structural analysis of β-glucan was confirmed using FT-IR. The FT-IR analysis was subjected to Nicolet™ iS™5 FTIR Spectrometer coupled with iD7 ATR accessory (Thermo Scientific™, Wisconsin, USA). Samples were analyzed with 32 scans and resolution at 4 cm−1 between wavelength 4000–525 cm−1. Ambient air was subtracted from all samples as a background by OMNIC software (version 9.3.32, Thermo Scientific, Massachusetts, USA) (https://www.thermofisher.com/order/catalog/product/833-036200 ).
9
Spectroscopic Characterization of Compounds
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The 1H, 13C NMR,
COSY, and HSQC spectra were collected with a Bruker 400 MHz spectrometer.
Electronic absorption spectroscopy was performed by using a PerkinElmer
Lambda 6 spectrophotometer in a 1 × 1 cm2 quart cuvette.
Fourier transform infrared (FTIR) spectra of compounds were obtained
between 500 and 4000 cm–1 by a Nicolet iS5 Spectrometer
equipped with an iD7 ATR accessory (Thermo Fischer Scientific Inc).
HPLC analysis was performed on a Water Alliance 2690 HPLC equipped
with a Waters 2487 Abs UV–vis detector set at 270 nm and a
KROMASIL 100 Å C–18 150 × 4.6 column. In the determination
of inhibitory concentration, the OD 600 values were registered by
using an Infinite Pro 200 plate reader (Tecan, Switzerland); the experiments
performed under anaerobic conditions were conducted in an anaerobiosis
jar (Oxoid jar with Anaerogen 2.5 L), with the system described in
paragraph 4 ofSI .
COSY, and HSQC spectra were collected with a Bruker 400 MHz spectrometer.
Electronic absorption spectroscopy was performed by using a PerkinElmer
Lambda 6 spectrophotometer in a 1 × 1 cm2 quart cuvette.
Fourier transform infrared (FTIR) spectra of compounds were obtained
between 500 and 4000 cm–1 by a Nicolet iS5 Spectrometer
equipped with an iD7 ATR accessory (Thermo Fischer Scientific Inc).
HPLC analysis was performed on a Water Alliance 2690 HPLC equipped
with a Waters 2487 Abs UV–vis detector set at 270 nm and a
KROMASIL 100 Å C–18 150 × 4.6 column. In the determination
of inhibitory concentration, the OD 600 values were registered by
using an Infinite Pro 200 plate reader (Tecan, Switzerland); the experiments
performed under anaerobic conditions were conducted in an anaerobiosis
jar (Oxoid jar with Anaerogen 2.5 L), with the system described in
paragraph 4 of
10
FTIR Analysis of Nanoemulsion Formulations
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A Nicolet IS 50 FTIR spectrometer equipped with an iD7 ATR accessory (Thermo Scientific Fisher, Waltham, MA, USA) was used to perform spectroscopic measurements with a background run used as a negative control. The spectra used ranged from 400 to 4000 cm−1 with a resolution of 4 cm−1 and 32 scans. The samples investigated by FTIR were a blank nanoemulsion of EO, blank nanoemulsion of EU, MUP dissolved in MeOH nanoemulsion of EO- and EU-loaded MUP.
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