Xanthine sodium salt

Xanthine sodium salt, xanthine oxidase enzyme, cytochrome C from horse heart, nitroblue tetrazolium (NBT), dimethyl sulfoxide (DMSO), phenyl methosulfate (PMS), phenymethylsulfonyl fluoride (PMSF), 1,4 dithiothreitol (DTT), 1,10 phenanthroline, trypan blue dye, bovine serum albumin (BSA), blue dextran, crystal violet, Sephacryl S-300, DEAE cellulose, and kits of gel filtration molecular weight marker were product of Sigma Co. SDS molecular weight marker proteins were purchased from Pharmacia Co., DMEM, fetal bovine serum, HEPES buffer solution, RPMI-1640, gentamycin, and L-glutamine are purchased from Lonza, Belgium.

Xanthine sodium salt (XSS, CAS:1196-43-6), bovine milk XOD (CAS:9002-17-9), APL (CAS:315-30-6), and formic acid (CAS:67-56-1, MS grade) were provided by Sigma Chemical Co., LTD (Shanghai, China). Methanol (CAS:67-56-1) and acetonitrile (CAS:75-05-8) (all MS grade) were offered by CNW Technologies (Shanghai, China). The 2-chloro-L-phenylalanine (CAS:103616-89-3, MS grade) was bought from Shanghai Hengbai Biotechnology Co., LTD (Shanghai, China). Ethanol absolute was purchased from Chengdu Jinshan Chemical Reagent Co., LTD (Chengdu, China). 10 × PBS buffer (pH 7.4) was offered by Shanghai Thermo Fisher (Shanghai, China). The gym was obtained from the Tibet specialty warehouse naicang supermarket (Tibet, Lhasa, China).

Xanthine, uric acid, hypoXanthine, adenosine, adenine and inosine were purchased from Yuanye Bio-Technology Co. Ltd. (Shanghai). Xanthine sodium salt and 8-azaXanthine monohydrate were purchased from Sigma-Aldrich.