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Elisas

Manufactured by Thermo Fisher Scientific
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ELISAs (Enzyme-Linked Immunosorbent Assays) are a type of analytical biochemistry technique used to detect and quantify specific substances, typically proteins, in a liquid sample. The core function of ELISAs is to measure the concentration of a target analyte in a sample by using antibodies and color changes.

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Lab products found in correlation

Cobas 6000, by Roche (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Taqman probe, by Thermo Fisher Scientific (1 mentions) Gm csf, by Thermo Fisher Scientific (1 mentions) Cobas mira plus, by Roche (1 mentions) Ab38994, by Abcam (1 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (1 mentions) Macs column, by Miltenyi Biotec (1 mentions) 8 oh dg, by Elabscience (1 mentions) Ripa buffer, by Beyotime (1 mentions) Griess reagent, by Merck Group (1 mentions) F4 80, by Bio-Rad (1 mentions) Multiplex assay, by Bio-Rad (1 mentions) Alexa fluor 647, by Bio-Rad (1 mentions) Ready set go, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

KC and MIP-2 ELISAs Caspase-3 Human Instant ELISA Kit Mouse Myeloperoxidase ELISA Kit Complement C3a Human ELISA Kit PrioCHECK FMDV NS Antibody ELISA Leptin Rat ELISA Kit Varioscan ELISA reader Human IL-17 Quantikine ELISA kits Quantitative ELISA kits BMP-2 ELISA kit

35 protocols using elisas

1

Cytokine Profiling in BA-Infected hDCs

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Cell-free culture fluids from control and BA infected hDCs were analyzed for secreted IL-1β, IL-6, IL-12p70 and IL-23 heterodimer by ELISAs (eBioscience) following the manufacturer’s protocols. Supernatants from primary and secondary exposure of T helper cells to uninfected and infected hDCs were analyzed for IL-17A and IFN-γ by ELISAs (eBioscience) following the manufacturer’s protocols.
Harris K.M., Ramachandran G., Basu S., Rollins S., Mann D, & Cross A.S. (2014). The IL-23/Th17 axis is involved in the adaptive immune response to B. anthracis in humans. European journal of immunology, 44(3), 752-762.
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2

Cytokine Production Analysis in BAL

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Production of the cytokines TNF-α, IL-8, and IL-6 were determined from first lavage BAL using enzyme-linked immunosorbent assays (ELISAs) according to the manufacturers protocol (Life Technologies, Grand Island, NY) and samples were run in duplicate.
Dunnick K.M., Morris A.M., Badding M.A., Barger M., Stefaniak A.B., Sabolsky E.M, & Leonard S.S. (2016). Evaluation of the effect of valence state on cerium oxide nanoparticle toxicity following intratracheal instillation in rats. Nanotoxicology, 10(7), 992-1000.
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3

Biomarker Analysis in Euthanized Rats

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Blood was sampled from the heart at euthanisation, processed and stored at −80 °C until analysis. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), bilirubin, albumin and haptoglobin levels were measured using Cobas 6000 (Roche Diagnostics, Hvidovre, Denmark). Serum levels of IL-6 and TNF-α were determined using commercial enzyme-linked immunosorbent assays (ELISAs; Life Technology, Naerum, Denmark). Rat acute phase protein alpha-2-macroglobulin (α2M) was detected using a quantitative ELISA-based test kit (Consultants Laboratory, Portland, USA). All assays were performed as specified by the manufacturers' instructions.
Jepsen B.N., Andersen K.J., Knudsen A.R., Nyengaard J.R., Hamilton-Dutoit S., Svendsen P., Etzerodt A., Møller H.J., Moestrup S.K., Graversen J.H, & Mortensen F.V. (2015). Anti-inflammatory liposomes have no impact on liver regeneration in rats. Annals of Medicine and Surgery, 4(4), 452-461.
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4

Biochemical and Cytokine Analysis of Serum

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Blood was centrifuged at 1500 × g for 10 min to collect clear serum and analysed within 2 h. The serum activities of alanine transaminase (ALT), aspartate aminotransferase (AST), kinase isoenzyme‐MB (CK‐MB) and lactate dehydrogenase (LDH) were measured using the ALT, AST, CK‐MB and LDH Reagent Kits (Jiancheng, Nanjing, Jiangsu, China), respectively. The serum levels of tumour necrosis factor‐α (TNF‐α), interleukin‐1β (IL‐1β) and interleukin‐6 (IL‐6) were measured by ELISAs (Thermo Fisher Scientific) according to the manufacturer's instructions.
Zhang G., Huang X., Xiu H., Sun Y., Chen J., Cheng G., Song Z., Peng Y., Shen Y., Wang J, & Cai Z. (2020). Extracellular vesicles: Natural liver‐accumulating drug delivery vehicles for the treatment of liver diseases. Journal of Extracellular Vesicles, 10(2), e12030.
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5

Fecal Neopterin Quantification Protocol

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Stool aliquots were extracted into a saline solution as published previously [29 (link)] and fecal neopterin was quantified from the extracts with ELISAs (B·R·A·H·M·S/ThermoFisher, Hennigsdorf, Germany; cat number 14-HD-99.1).
Bouzid Y.Y., Chin E.L., Spearman S.S., Alkan Z., Stephensen C.B, & Lemay D.G. (2023). No Associations between Dairy Intake and Markers of Gastrointestinal Inflammation in Healthy Adult Cohort. Nutrients, 15(16), 3504.
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6

Lung Gene Expression and Cytokine Analysis

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RNA from lungs was extracted with Trizol (Invitrogen). Gene expression was calculated using ΔΔCT method relative to naïve sample using TaqMan probes (Applied Biosystems) and normalized to GAPDH. IFN-γ, IFN-λ, and TNF-α cytokines in lung homogenates were measured by ELISAs (ThermoFisher and R&D systems).
Blais B.S., Shouval H.Z, & Cooper L.N. (1999). The role of presynaptic activity in monocular deprivation: Comparison of homosynaptic and heterosynaptic mechanisms. Proceedings of the National Academy of Sciences of the United States of America, 96(3), 1083-1087.
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7

Bone Marrow Dendritic Cell Responses to Bacterial Cells

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Bacterial cells were grown in defined medium as above for 24 and 48 h. Cultures were centrifuged and bacterial pellets were resuspended in 1× PBS and corrected to an optical density measured at 600 nm (OD600) of 1.0. Bone marrow was isolated from C57BL/6 mice and grown in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) (PreProTech) for 7 d. Once matured, the BMDCs were scraped and plated in 96-well plates and allowed to adhere. After at least 3 h, BMDCs were treated with whole bacterial cells overnight. Additionally, BMDCs were treated with pure capsule preparation overnight and then, the following morning, were treated with lipopolysaccharide (LPS) for 1.5 h. TNF-α production was detected using ELISAs (ThermoFisher) as described previously (16 (link)).
Henke M.T., Brown E.M., Cassilly C.D., Vlamakis H., Xavier R.J, & Clardy J. (2021). Capsular polysaccharide correlates with immune response to the human gut microbe Ruminococcus gnavus. Proceedings of the National Academy of Sciences of the United States of America, 118(20), e2007595118.
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8

Quantifying IL-17 and IFNγ in LPMC

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Levels of IL-17 and IFNγ in LPMC culture supernatants were quantified using ELISAs (ThermoFisher). Assays were carried out according as per manufacturers protocols (Analytical sensitivity: IL-17 4pg/ml, IFNγ 4pg/ml).
Kibbie J.J., Dillon S.M., Thompson T.A., Purba C.M., McCarter M.D, & Wilson C.C. (2021). Butyrate directly decreases human gut lamina propria CD4 T cell function through histone deacetylase (HDAC) inhibition and GPR43 signaling.. Immunobiology, 226(5), 152126.
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9

Evaluating Cytokine Modulation by Drug-Loaded ZIF-8

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RAW264.7 cells were seeded into a 24-well plate (5 × 105 cells/well) and incubated with LPS at a final concentration of 10 µg/mL at 37 °C for 24 h. The culture medium was then replaced with fresh medium containing free MTX, MTX@ZIF-8, MV/MTX@ZIF-8, FPD/MV/MTX@ZIF-8, MV/ZIF-8 or FPD/MV/ZIF-8 at a final MTX concentration of 20 µg/mL. Cells cultured in PBS-containing medium were used as negative controls. After incubation at 37 °C for 24 h, the culture medium was collected and centrifuged at 2000×g for 5 min. The levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-10 in the supernatant were determined using commercial enzyme-linked immunosorbent assays (ELISAs, Thermo Fisher, Austria) following the manufacturer’s instructions [49 (link)].
Wang Y., Jia M., Zheng X., Wang C., Zhou Y., Pan H., Liu Y., Lu J., Mei Z, & Li C. (2022). Microvesicle-camouflaged biomimetic nanoparticles encapsulating a metal-organic framework for targeted rheumatoid arthritis therapy. Journal of Nanobiotechnology, 20, 253.
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10

Cytokine Secretion Quantification by ELISA

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Cytokine secretion for TNF-α, IL-6, and CCL2 was determined using enzyme-linked immune sorbent assay kits (ELISAs) from Peprotech (Hamburg, Germany), following the manufacturer’s instructions (Article numbers: TNF-α: 900-K25; IL-6: 900-K16; CCL2: 900-K31).
Rinderknecht H., Mayer A., Histing T., Ehnert S, & Nüssler A. (2023). Herbal Extracts of Ginseng and Maqui Berry Show Only Minimal Effects on an In Vitro Model of Early Fracture Repair of Smokers. Foods, 12(15), 2960.
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