Human recombinant egf

Manufactured by Abcam

Human recombinant EGF is a protein product that represents the epidermal growth factor (EGF) derived from human cells. EGF is a signaling molecule that plays a role in cell growth, proliferation, and differentiation.

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Lab products found in correlation

Mepi500ca, by Thermo Fisher Scientific (1 mentions) 3 isobutyl 1 methyl xanthine (ibmx), by Abcam (1 mentions) Gentamicin amphotericin, by Thermo Fisher Scientific (1 mentions) 3 isobutyl 1 methylxanthine ibmx, by Merck Group (1 mentions) Bovine pituitary extract, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Abcam (1 mentions) Hydrocortisone, by Tokyo Chemical Industry (1 mentions) Cnt prime, by CELLnTEC (1 mentions)

Spelling variants of this product

Recombinant human EGF protein (2 citations)

2 protocols using human recombinant egf

Optimized Cell Culture Media for Urinary Epithelial Cells

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High-glucose DMEM supplemented with 10% FBS (Gibco), MEM non-essential amino acids, 100 mM sodium pyruvate and 100 U/mL penicillin-streptomycin was used as “Standard Medium”. CnT-Prime was purchased from CELLnTEC. “Uromedium” was prepared according to Osborn et al.^{6 (link)} with slight modifications. Briefly, “Uromedium” was comprised of EpiLife medium with 60 µM calcium (Gibco; MEPI500CA) supplemented with 60 μg/mL bovine pituitary extract (Gibco), 5 μg/mL human recombinant insulin (Diagnocine), 500 ng/mL hydrocortisone (Tokyo Chemical Industry), gentamicin/amphotericin (Gibco), 2% FBS, 0.1 ng/mL human recombinant epidermal growth factor (EGF) (RSD) and 100 μM 3-Isobutyl 1-methylxanthine (IBMX) (Sigma-Aldrich). “UCM” was comprised of EpiLife medium with 60 µM calcium supplemented with 60 μg/mL bovine pituitary extract, 5 μg/mL human recombinant insulin, gentamycin/amphotericin, 2% FBS, 0.01 ng/mL human recombinant EGF, 1 mM IBMX and 1 μM tranylcypromine (Abcam).

Inoue Y., Kishida T., Kotani S.I., Akiyoshi M., Taga H., Seki M., Ukimura O, & Mazda O. (2019). Direct conversion of fibroblasts into urothelial cells that may be recruited to regenerating mucosa of injured urinary bladder. Scientific Reports, 9, 13850.

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Scratch Wound Assay for RPE Cells

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Control or stable KDM4A overexpression RPE cells were plated in 6 well tissue culture plates at a density of 2×10⁵. Cells were allowed to adhere to plates for 24 hours. After 24 hours in culture, a p200 pipette tip was used to introduce a scratch wound in the centre of the well from the 12 o’clock to 6 o’clock position. Following the induction of the scratch wound, media was removed from each well and 1ml of DMEM was used to rinse the wells, removing any cellular debris. After this wash, 3ml of DMEM supplemented with vehicle or human recombinant EGF (Abcam) to a final concentration of 50ng/ml, was added to each well. Cells were imaged at 0hrs, 12hrs and 24hrs, using the EVOS imaging platform at 4x magnification. Scratch wound measurements were performed using the EVOS software with a minimum of 5 measurements taken at various locations, per scratch wound. All measurements were averaged. Each condition was performed in triplicate for each independent experiment.

Clarke T.L., Tang R., Chakraborty D., Rechem C.V., Ji F., Mishra S., Ma A., Kaniskan H.Ü., Jin J., Lawrence M.S., Sadreyev R.I, & Whetstine J.R. (2019). Histone Lysine Methylation Dynamics Control EGFR DNA Copy Number Amplification. Cancer discovery, 10(2), 306-325.

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