Serum concentrations of cytokines: IL-1α, IL-1β, IL-6, IL-10, IL-12p70, IL-17α, IL-23, IL-27, MCP-1, interferon-β (IFN-β), IFN-γ, tumor necrosis factor-α (TNF-α), and GM-CSF were measured using LEGENDplex Mouse Inflammation Panel (13-plex) immunoassay (Biolegend; San Diego, CA, USA) and BD LSRFortessa flow cytometer and analyzed using LEGENDplex software (Biolegend).
Legendplex mouse inflammation panel 13 plex immunoassay
Manufactured by BioLegend
Sourced in United States
The LEGENDplex Mouse Inflammation Panel (13-plex) immunoassay is a multiplex assay designed to simultaneously measure the levels of 13 different mouse inflammation-related protein targets in a single sample. The assay utilizes bead-based technology and flow cytometry analysis to provide quantitative data on the concentrations of these analytes.
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Lab products found in correlation
2 protocols using legendplex mouse inflammation panel 13 plex immunoassay
1
Multiplex Cytokine Profiling in Mice
2
Comprehensive Euthanasia Blood Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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On the day of euthanasia, blood
was first taken from the facial vein for hematological analyses performed
with a fully automated veterinary analyzer ABC Vet (HORIBA, UK): counts
of white blood cells, red blood cells, platelets, granulocytes, lymphocytes
(LYM), and monocytes; hematocrit value, mean corpuscular volume; hemoglobin
concentration; and red blood cell distribution width. Next, blood
was drawn postmortem by cardiac puncture, allowed to clot, and centrifuged
(800g, 10 min, RT). Serum isolated by centrifugation
(800g, 10 min, RT) was used for biochemical analysis
(using a SPOTCHEM EZ Chemistry Analyzer—Woodley Equipment and
multiparameter strips SPOTCHEM II Panel V, according to the manufacturer’s
instructions) and cytokine profiling. The levels of IL-1α, IL-1β,
IL-6, IL-10, IL-12p70, IL-17α, IL-23, IL-27, MCP-1, IFN-β,
IFN-γ, TNF-α, and GM-CSF were examined using the LEGENDplex
Mouse Inflammation Panel (13-plex) immunoassay (BioLegend) and a BD
LSRFortessa flow cytometer, and analyzed using LEGENDplex software
(BioLegend).
was first taken from the facial vein for hematological analyses performed
with a fully automated veterinary analyzer ABC Vet (HORIBA, UK): counts
of white blood cells, red blood cells, platelets, granulocytes, lymphocytes
(LYM), and monocytes; hematocrit value, mean corpuscular volume; hemoglobin
concentration; and red blood cell distribution width. Next, blood
was drawn postmortem by cardiac puncture, allowed to clot, and centrifuged
(800g, 10 min, RT). Serum isolated by centrifugation
(800g, 10 min, RT) was used for biochemical analysis
(using a SPOTCHEM EZ Chemistry Analyzer—Woodley Equipment and
multiparameter strips SPOTCHEM II Panel V, according to the manufacturer’s
instructions) and cytokine profiling. The levels of IL-1α, IL-1β,
IL-6, IL-10, IL-12p70, IL-17α, IL-23, IL-27, MCP-1, IFN-β,
IFN-γ, TNF-α, and GM-CSF were examined using the LEGENDplex
Mouse Inflammation Panel (13-plex) immunoassay (BioLegend) and a BD
LSRFortessa flow cytometer, and analyzed using LEGENDplex software
(BioLegend).
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