The siRNAs specifically targeting human cyclin D1 mRNA (Hs_CCND_1, Hs_CCND_2, Hs_CCND_3), purchased from Qiagen, were used for suppressing endogenous cyclin D1 expression in HT1080 cells. For suppression of endogenous cyclin D1 expression in G9a−/− MEFs rescued with wild type G9a or vector control, mouse Ccnd1_2 FlexiTube siRNAs (Catalog #SI00943642) specifically targeting mouse cyclin D1 mRNA were also purchased from Qiagen. The cyclin D1 siRNAs or negative control siRNA (Qiagen) were transfected into the cells with the Lipofectamine 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer’s instruction. For HT1080 cells which stably expressing the Shield1-inducible Dam-Lamin B1 and the Tet-off m6A-Tracer, after transfection, the cells were sequentially treated with 10 μM Nocodazole (Sigma) for 72 hours and 0.5 μM Shield1 (Clontech) in DMEM with 10% tetracycline-free FBS for 2 hours to induece Dam-Lamin B1 and m6A-Tracer expressing. The immunofluorescence staining were performed another 24 hours later. For G9a−/− MEFs, immunofluorescence staining were processed 72 hours later after siRNA transfection.
Mouse ccnd1 2 flexitube sirnas
Manufactured by Qiagen
Mouse Ccnd1_2 FlexiTube siRNAs are a set of small interfering RNAs (siRNAs) targeting the Ccnd1 gene in mice. The Ccnd1 gene encodes the cyclin D1 protein, which is involved in cell cycle regulation. These siRNAs are designed to facilitate the study of Ccnd1 gene function and its role in cellular processes.
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2 protocols using mouse ccnd1 2 flexitube sirnas
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Cyclin D1 siRNA Knockdown Assay
2
Cyclin D1 Knockdown Techniques
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The siRNAs specifically targeting human cyclin D1 mRNA (Hs_CCND_1, Hs_CCND_2, and Hs_CCND_3), purchased from Qiagen, were used for suppressing endogenous cyclin D1 expression in HT1080 cells. For suppression of endogenous cyclin D1 expression in G9a−/− MEFs rescued with wild-type G9a or vector control, mouse Ccnd1_2 FlexiTube siRNAs (Catalog #SI00943642) specifically targeting mouse cyclin D1 mRNA were also purchased from Qiagen. The cyclin D1 siRNAs or negative control siRNA (Qiagen) were transfected into the cells with the Lipofectamine 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer’s instruction. For HT1080 cells, which stably expressing the Shield1-inducible Dam-Lamin B1 and the Tet-off m6A-tracer, after transfection, the cells were sequentially treated with 10 μM Nocodazole (Sigma) for 72 h and 0.5 μM Shield1 (Clontech) in DMEM with 10% tetracycline-free FBS for 2 h to induce Dam-Lamin B1 and m6A-tracer expressing. The immunofluorescence staining was performed another 24 h later. For G9a−/− MEFs, immunofluorescence staining was processed 72 h later after siRNA transfection.
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