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100 nm liposomes

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The 100 nm liposomes are a type of lipid-based nanoparticle with an average diameter of 100 nanometers. These liposomes are composed of phospholipids and can be used as a platform for various applications, including drug delivery, diagnostics, and research. The core function of the 100 nm liposomes is to provide a controlled and stable environment for the encapsulation and delivery of various payloads.

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Lab products found in correlation

Ivis lumina series 3 imaging system, by PerkinElmer (2 mentions)

2 protocols using 100 nm liposomes

1

Tracing Organ Uptake of DiD-labeled EVs

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20 μg of DiD-labeled EVs were injected via tail-vein into each C3H/HeJ mouse. 24 hours after the injection, the mice were euthanized and the organs were harvested. The organs were imaged with an IVIS Lumina Series III Imaging System (PerkinElmer) to detect the fluorescence of DiD-labeled EVs uptaken by the organs. The imaging for PBS, control and IGF2BP1 groups were repeated twice with n=>3 mice per group. The spleen and bone marrow cells were isolated from each mouse and single cell suspensions were prepared. Each sample was then run in a flow cytometer and cells with the DiD-labeled EVs were detected in the APC channel with red laser excitation. 100 nm liposomes (Encapsula NanoSciences LLC) were also labeled with DiD and injected into the mice as control. Furthermore, EVs from parent SW1 cells (without the shIGF2BP1) were used as controls for this experiment, with or without doxycycline treatment.
Ghoshal A., Rodrigues L.C., Gowda C.P., Elcheva I.A., Liu Z., Abraham T, & Spiegelman V.S. (2019). Extracellular vesicle-dependent effect of RNA-binding protein IGF2BP1 on melanoma metastasis. Oncogene, 38(21), 4182-4196.
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2

Tracing Organ Uptake of DiD-labeled EVs

Check if the same lab product or an alternative is used in the 5 most similar protocols
20 μg of DiD-labeled EVs were injected via tail-vein into each C3H/HeJ mouse. 24 hours after the injection, the mice were euthanized and the organs were harvested. The organs were imaged with an IVIS Lumina Series III Imaging System (PerkinElmer) to detect the fluorescence of DiD-labeled EVs uptaken by the organs. The imaging for PBS, control and IGF2BP1 groups were repeated twice with n=>3 mice per group. The spleen and bone marrow cells were isolated from each mouse and single cell suspensions were prepared. Each sample was then run in a flow cytometer and cells with the DiD-labeled EVs were detected in the APC channel with red laser excitation. 100 nm liposomes (Encapsula NanoSciences LLC) were also labeled with DiD and injected into the mice as control. Furthermore, EVs from parent SW1 cells (without the shIGF2BP1) were used as controls for this experiment, with or without doxycycline treatment.
Ghoshal A., Rodrigues L.C., Gowda C.P., Elcheva I.A., Liu Z., Abraham T, & Spiegelman V.S. (2019). Extracellular vesicle-dependent effect of RNA-binding protein IGF2BP1 on melanoma metastasis. Oncogene, 38(21), 4182-4196.
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