A powder X-ray diffraction (PXRD) pattern was obtained to perform crystal structure analysis using BRUKER (D2 Phaser) with Ni-filtered Cu-Kα irradiation (λ = 1.5406 Å) over 2θ range from 5° to 50°. FEI NOVA Nano 450 scanning electron microscope (SEM) equipped with an energy dispersive X-ray spectroscope (EDX) was used to analyze the morphology of the samples. The samples’ Zeta potential (ZP) was obtained through Zetasizer (Nano ZS, Malvern) at room temperature in water. N2 adsorption-desorption isotherm was obtained to Brunauer-Emmett-Teller (BET) surface area and porous makeup of the samples using Quantachrome Nova 2200e. Infrared studies were performed using Bruker Alpha Platinum ATR between the 500–4,500 cm-1 range. Thermogravimetric analysis (TGA) was obtained through the TA instrument under an N2 atmosphere in a temperature ranging from 10°C to 600°C with a heat ramp of 10°/min. UV-Vis spectrophotometry was used to characterize drug loading/release and TMB oxidation studies by Shimadzu UV-1800 spectrophotometer. The cellular uptake fluorescence studies were performed through confocal laser scanning microscope (CLSM) model ZEISS LSM—880, Jena, Germany.
Alpha platinum atr
Manufactured by Bruker
Sourced in United States
The Alpha Platinum ATR is a Fourier Transform Infrared (FTIR) spectrometer designed for Attenuated Total Reflectance (ATR) analysis. It provides accurate and reliable infrared spectroscopy measurements for a wide range of solid, liquid, and semi-solid samples.
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Lab products found in correlation
Avance 500 mhz spectrometer, by Bruker (3 mentions)
Toluene, by Thermo Fisher Scientific (2 mentions)
Acs grade methanol, by Thermo Fisher Scientific (2 mentions)
Anhydrous dmf, by Thermo Fisher Scientific (2 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (2 mentions)
Avance 3 400, by Bruker (2 mentions)
Sdt q600, by TA Instruments (2 mentions)
Uv 1800 spectrophotometer, by Shimadzu (1 mentions)
N methyl pyrrolidone (nmp), by Thermo Fisher Scientific (1 mentions)
Apex 2 ccd detector, by Bruker (1 mentions)
Microstar, by Bruker (1 mentions)
Opus software version 7, by Bruker (1 mentions)
Cary 60 uv vis spectrometer, by Agilent Technologies (1 mentions)
Tecnai 12 g2 spirit biotwin, by Thermo Fisher Scientific (1 mentions)
Zetasizer nano z, by Malvern Panalytical (1 mentions)
Cary eclipse fluorescence spectrometer, by Agilent Technologies (1 mentions)
S 3000, by Hitachi (1 mentions)
Sz 100, by Horiba (1 mentions)
Opus software, by Bruker (1 mentions)
Spectrospin spectrometer, by Bruker (1 mentions)
27 protocols using alpha platinum atr
1
Comprehensive Physicochemical Characterization of Materials
2
Characterization of Synthesized Silver Nanoparticles
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Fourier transform infrared spectroscopy (FTIR) was used to study the organic functional groups attached to the surface of AgNPs. The synthesized nanoparticles were purified and dried at 60 °C. The dried samples were mixed with a fine powder of potassium bromide (KBr) and analyzed by FTIR (Bruker Alpha Platinum ATR). The size surface charge of silver nanoparticles was determined using a zeta sizer. Particle size measurement was based on the time-dependent fluctuation of laser light scattering by the nanoparticles undergoing Brownian motion78 .
3
Synthesis and Characterization of ITZ Analogues
4
FTIR Profiling of DM1 Fibroblasts
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The DM1-derived fibroblasts and controls spectra were acquired using a FTIR spectrometer (Alpha Platinum ATR, Bruker Corporation, Billerica, MA, USA) equipped with a diamond ATR crystal, and preprocessed using OPUS software version 7.0 (Bruker Corporation, Billerica, MA, USA) Five µL of the DM1-derived fibroblasts and controls were placed in the crystal. To overcome the water interference, DM1-derived fibroblasts were air-dried before spectra were acquired. The spectra were obtained in the wavenumber range 4000–600 cm−1, with a resolution of 8 cm−1 and 64 co-added scans. Three replicates were obtained from DM1-derived fibroblasts and controls. Between different samples reading, the crystal was cleaned with 70% ethanol and distilled water and a background spectrum was acquired with the crystal empty (cleaned) to eliminate possible interference from fluctuations in the conditions of the room. All spectra acquisition was performed in a controlled room with a temperature of 23 °C and humidity of 35%.
5
Characterization of Py-P3HT Nanoparticles
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Ultraviolet-visible (UV-vis) absorption spectra were recorded on Cary 60 UV-vis spectrometer (Agilent). Fluorescence spectra were recorded on a Cary Eclipse Fluorescence Spectrometer (Agilent). The UV-vis and fluorescence spectra of all samples were measured by using a quartz cuvette (4 mL). Fourier-transform infrared spectra (FTIR) were recorded on a Bruker FTIR spectrometer (ALPHA Platinum-ATR). Py-P3HT NPs sample for FTIR characterization was prepared by dropping the aqueous Py-P3HT NPs solution (20μL) to the top of glass, which was repeated over times after the solvent was evaporated by dry nitrogen gas flow, the red solid power was then collected from the surface of glass and subject to testing. Dynamic light scattering and Zeta potential was tested on Zetasizer Nano ZS (Malvern Instrument). Hydrodynamic diameter (Dh) of NPs was measured by DLS at scattering angle θ of 173°. Transmission electron microscopy (TEM) images were recorded on FEI Tecnai 12 G2 Spirit BioTWIN. Lifetime measurement was conducted at a Mini-tau lifetime spectrometer (Edinburgh Instruments). The TEM sample were prepared by dropping the Py-P3HT NP solution to the TEM gird placed on the top of a filter paper, which stand at room temperature for 1 h to fully evaporate the solvent.
6
Comprehensive Nanoparticle Characterization
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The characterization of the synthesized nanoparticles was carried out using different characterization techniques. The crystalline phases of the nanoparticles obtained after calcination were analyzed by X-ray diffraction (XRD), using an X’Pert PRO PANalytical instrument with Cu kα = 1.54056, 20 kV, that scanned from 5° to 80° at 2°/min scanning speed. The functional groups and the vibrational band of bonds were determined by Fourier Transformed Infrared spectroscopy (FTIR) using a Bruker Alpha-Platinum ATR instrument with 40 scans and resolution of 4 cm−1 measured between a wavelength of 4000 to 400 cm−1. Scanning electron microscopy (SEM; HITACHI S-3000) was used to determine the microstructure and morphology of the nanoparticles with an energy-dispersive X-ray spectrometer (EDX) used to analyze the component and sample purity of the nanoparticles. The mean particle size and zeta potential of the nanoparticles were measured using Horiba Scientific, SZ 100 instrument.
7
ATR-FTIR Analysis of Plasma Proteins
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FTIR spectra were acquired using an ATR-FTIR spectrometer (Alpha Platinum ATR, Bruker, Billerica, MA, EUA), and pre-processed using OPUS software (Bruker, Billerica, MA, EUA). All spectra were recorded in the medium infrared region (4000–600 cm−1), with a resolution of 8 cm−1 and 64 co-added scans. A background spectrum was acquired with the crystal empty before each sample measurement. Each plasma sample (5 μL) was spotted into the crystal and air-dried prior to analysis in a room with controlled temperature (23 °C) and relative humidity (35%). Between each measurement, the crystal was cleaned with ethanol 70% and distilled water and dried to avoid cross-contamination and interferences in the spectra. Spectra were baseline corrected and normalized to amide I band. To resolve overlapping bands, a second-derivative analysis with a Savitzky–Golay algorithm and 3 smoothing points was performed. Principal Component Analysis (PCA) was applied to the 1700–1600 cm−1 spectral region, assigned to amide I band of proteins, to assess differences between groups related to protein secondary structures.
8
Spectroscopic Analysis of Organometallic Compounds
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UV–visible absorption spectra
were recorded using a Varian CARY 50 Bio UV–visible spectrophotometer
at 298 K using 1 cm path length quartz cuvettes. All infra-red spectra
were recorded on a Bruker Alpha Platinum ATR. All NMR spectra were
recorded on a 400 MHz Bruker Spectrospin spectrometer using deuterated
solvents. Chemical shifts are reported as δ in parts per million
using the residual protonated solvent as internal standard.31 (link)
were recorded using a Varian CARY 50 Bio UV–visible spectrophotometer
at 298 K using 1 cm path length quartz cuvettes. All infra-red spectra
were recorded on a Bruker Alpha Platinum ATR. All NMR spectra were
recorded on a 400 MHz Bruker Spectrospin spectrometer using deuterated
solvents. Chemical shifts are reported as δ in parts per million
using the residual protonated solvent as internal standard.31 (link)
9
Characterization of Colloidal Samples
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ATR-FTIR spectra of dry samples were recorded on a Bruker FTIR (Fourier Transform Infrared Spectroscopy) Alpha Platinum ATR spectrophotometer (Bruker, Billerica, Massachusetts, USA). Samples were dried on to the surface of the ATR diamond crystal prior to measurements. Dynamic light scattering was measured using Malvern Zetasizer NanoZS (Malvern Industries, Malvern, UK) with the non-invasive backscatter algorithm. Z-average size, polydispersity index (PDI), and hydrodynamic diameter based on number distribution of particles are reported. Zeta potential was also measured on Zetasizer NanoZS, with disposable folded capillary cells and the M3-PALS measurements technology. The measurement was conducted after 5 min of stirring of colloidal suspension in the sample cell. The refractive index of 1.10 was used.
10
Comprehensive Characterization of Metal-Organic Frameworks
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The X-ray diffraction
(XRD) pattern of the samples was obtained on a BRUKUER (D2 Phaser)
diffractometer over a 2θ range from 5 to 80° using Ni-filtered
Cu Kα irradiation (λ = 1.5406 Å). The surface morphology
of the MOFs was characterized through an FEI NOVA Nano SEM 450 scanning
electron microscope (SEM) equipped with an energy dispersive X-ray
spectroscope (EDX). The nitrogen adsorption–desorption isotherm
was calculated using Quantachrome Nova 2200e. The FT-IR spectra of
the samples were assessed in the range of 400–4000 cm–1 using a Bruker Alpha Platinum ATR instrument. Thermogravimetric
analysis (TGA) was performed under an N2 atmosphere in
the temperature range of 10 to 600 °C (10°/min ramp) using
the TA Instruments (SDT Q600). The particle size of the MOFs was determined
on a Malvern Zetasizer (Nano ZS, Malvern) using dynamic light scattering
(DLS) at room temperature. The magnetic properties of MIL-88B (Fe)
and FeMn-MIL-88B were analyzed by a physical magnetic system and vibrating-sample
magnetometer (Cryogenic Ltd.). The high-performance liquid chromatography
(HPLC) system used was manufactured by The Waters Alliance (Model
e2695) and equipped with a (Waters 2998) photodiode array detector
and fitted with a C18 column. UV–vis spectroscopy was performed
to determine the drug content in the liquid samples using a spectrophotometer
(Shimadzu UV-1800).
(XRD) pattern of the samples was obtained on a BRUKUER (D2 Phaser)
diffractometer over a 2θ range from 5 to 80° using Ni-filtered
Cu Kα irradiation (λ = 1.5406 Å). The surface morphology
of the MOFs was characterized through an FEI NOVA Nano SEM 450 scanning
electron microscope (SEM) equipped with an energy dispersive X-ray
spectroscope (EDX). The nitrogen adsorption–desorption isotherm
was calculated using Quantachrome Nova 2200e. The FT-IR spectra of
the samples were assessed in the range of 400–4000 cm–1 using a Bruker Alpha Platinum ATR instrument. Thermogravimetric
analysis (TGA) was performed under an N2 atmosphere in
the temperature range of 10 to 600 °C (10°/min ramp) using
the TA Instruments (SDT Q600). The particle size of the MOFs was determined
on a Malvern Zetasizer (Nano ZS, Malvern) using dynamic light scattering
(DLS) at room temperature. The magnetic properties of MIL-88B (Fe)
and FeMn-MIL-88B were analyzed by a physical magnetic system and vibrating-sample
magnetometer (Cryogenic Ltd.). The high-performance liquid chromatography
(HPLC) system used was manufactured by The Waters Alliance (Model
e2695) and equipped with a (Waters 2998) photodiode array detector
and fitted with a C18 column. UV–vis spectroscopy was performed
to determine the drug content in the liquid samples using a spectrophotometer
(Shimadzu UV-1800).
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