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5 protocols using phosphate buffer tablets

1

Pluronic F127-based Mesoporous Silica

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Triblock poly(ethylene oxide)-b-poly(propylene oxide)-bpoly(ethylene oxide) copolymer EO 106 PO 70 EO 106 (Pluronic F127, MW=12 600), tetraethoxysilane (TEOS, 99%), 1,3,5trimethylbenzene (TMB), 3-aminopropyltriethoxysilane (APTES, 99%), potassium chloride (KCl), phosphate buffer tablets and Tween 80 were purchased from Aldrich. A fluorocarbon surfactant (FC-4) was purchased from Yick-Vic Chemicals & Pharmaceuticals (HK) Ltd. All chemicals were used as received without purification.
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2

Ibuprofen Ester Synthesis Protocol

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Ibuprofen, dichloromethane, dimethylaminopyridine (DMAP), dicyclohexylcarboiimide (DCC), acetone, hexane, 2-hydroxyethylmethacrylate, ethylene glycol dimethacrylate, benzyl peroxide, phosphate buffer tablets, silica and silica plates were all obtained from Aldrich, Gillingham, Dorset, UK. dichloromethane (DCM), ethanol (EtOH), ethyl acetate (EtOAc), hexane, acetonitrile and acetone were all obtained from BDH Laboratories, Poole, Dorset, UK. All other chemicals used were of analytical reagent grade.
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3

Aptamer-based assay for NT-proBNP and cTnI

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Phosphate buffer tablets (PBS, 137 mM NaCl, 2.7 mM KCl, and 10 mM phosphate) and Tween were purchased from Sigma-Aldrich (Burlington, MA, USA). The 3′-azide-modified NT-proBNP aptamer (5′-NH2-GGCAGGAAGACAAACAGGTCGTAGTGGAAACTGTCCACCGTAGACCGGTTATCTAGTGGTCTGTGGTGCTGT-N3-3′) was purchased from DNA Synthesis, LLC (Moscow, Russia) and purified using denaturing polyacrylamide gel electrophoresis (PAGE) to reach a mass of 3.1 nM (69.5 µg). The azide group was introduced in the oligonucleotide during the synthesis using azide-modified controlled pore glass (CPG). Frozen solutions of 1.18 mg/mL of NT-proBNP in 10 mM potassium phosphate, pH 7.4, 150 mM NaCl, and 0.7 mg/mL of cTnI in 10 mM HCl were purchased from HyTest Ltd. (Turku, Finland).
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4

Synthesis of Flurbiprofen and Naproxen Derivatives

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Flurbiprofen, naproxen, anhydrous dichloromethane (DCM), dimethylaminopyridine (DMAP), dicyclohexylcarbodiimide (DCC), dimethylformamide (DMF) ethylene glycol dimethacrylate, 2-methoxyethyl acrylate, phosphate buffer tablets (pH 7.3 ± 0.2 @ 25°C), silica, silica plates and triethylamine were all purchased from Sigma-Aldrich (Gillingham, Dorset, UK). All other chemicals were of AnalaR or equivalent grade and purchased from BDH laboratories (Poole, Dorset, UK).
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5

Nutritional Composition and Antioxidant Properties of Lesser Mealworm

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The insect powder of lesser mealworm (A. diaperinus L.) containing 59.6% protein, 28.7% fat, 3.7% fiber, 2.7% carbohydrate, and 0.9% salt was purchased from Snack-Insects Co. (Witzeeze, Germany). The total content of protein, fat, and carbohydrate of LMLPs had been determined by the Kjeldahl, Soxhlet, and Bradford (the rapid colorimetrics of phenol-sulfuric acid with the standard of D-glucose) techniques, respectively. Ethanol, isopropanol, n-hexane, diethyl ether, methanol, toluene, iso-octane, cyclohexane, anhydrous sodium sulfate, sodium carbonate, iron (III) chloride, potassium hydroxide (KOH), potassium ferricyanide, hydrochloric acid (HCl), trichloroacetic acid (TCA), gallic acid (GA), 1,1- diphenyl-2-picrylhydrazyl (DPPH), Folin–Ciocalteu’s reagent, phenolphthalein, phosphate buffer tablets, and vitamin C were purchased from Sigma-Aldrich Chemical Co. (Darmstadt, Germany). All other chemicals used were of analytical grade and obtained from either Sigma-Aldrich or Merck Chemical Co. (Darmstadt, Germany).
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