Arterial blood gas analyses were performed immediately after collection of the blood samples. The remaining blood was centrifuged (15 min at 3000 rpm, 4 °C); the separated plasma was collected and stored at −70 °C until needed for the blood chemistry profile analyses. Serum lactic acid, albumin, amylase, alanine aminotransferase (ALT), and glucose levels were measured using a blood gas analyzer (RAPIDPoint®500 Blood Gas Systems, Siemens Healthcare Diagnostics, Munich, Germany) and a chemistry analyzer (VetScan VS2, Abaxis, Union City, CA, USA).
Rapidpoint 500 blood gas system
Manufactured by Siemens
Sourced in Germany, Canada
The RAPIDPoint 500 Blood Gas Systems is a laboratory equipment product manufactured by Siemens. It is designed to analyze blood samples and provide measurements of various blood parameters, such as pH, partial pressure of oxygen and carbon dioxide, and electrolyte levels. The core function of the RAPIDPoint 500 is to deliver accurate and reliable blood analysis results to support medical decision-making.
Automatically generated - may contain errors
9 protocols using rapidpoint 500 blood gas system
1
Blood Chemistry Profile Analysis
2
Media Characterization Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Media samples were taken with 1-mL syringes from both chambers daily to determine changes in pH, glucose, and lactate. RAPIDPoint 500 Blood Gas Systems (Siemens Healthcare Limited, Canada) was used to measure these parameters in media samples. This analyzer can measure pH, partial pressure of carbon dioxide and oxygen, and concentrations of many ions such as sodium, potassium, chloride, bicarbonate, and metabolites, namely calcium, magnesium, glucose, lactate.
3
Metabolic Monitoring of Cell Cultures
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In each experiment, we removed the plates from the respective incubator and took 200 μL media samples and measured glucose, lactate, oxygen, potassium, sodium, and calcium concentrations using RAPIDPoint 500 Blood Gas Systems (Siemens Healthcare Limited, Canada) six hours after seeding and then every 12 hours (Experiment 1), or 24 hours (Experiments 2, 3, and 4), with the last measurement taken at 114 hours (5 days). At the same intervals, we took five images of the wells (EVOS FL Cell Imaging System) to estimate the total cell count.
4
Arterial and Venous Sampling for Metabolic Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Arterial and venous samples were collected at baseline, following DCD induction, hourly during ESHP, and hourly following transplantation. Electrolyte, lactate and hemoglobin concentrations, pH, PO 2 , PCO 2 , and oxygen saturation were measured using a blood gas analyzer (RAPIDPoint 500 Blood Gas Systems; Siemens). All metabolic parameters were assessed in Langendorff mode.
5
Arterial and Venous Blood Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Arterial and mixed venous blood samples were collected for measurements of pH, lactate levels, pO 2 , hemoglobin, hematocrit, and oxygen saturation using a point-of-care blood gas analyzer (RAPIDPoint 500 Blood Gas Systems, Siemens).
6
Serum MDA and NGAL Biomarker Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum MDA concentration was determined by using enzyme-linked immunosorbent assay kit Cloud-Clone Corp. (Katy, TX, USA). The minimum detectable dose of malondialdehyde was typically less than 8.84 ng/mL.
For NGAL level measurements, Human Lipocalin-2/NGAL ELISA kit Biovendor (Brno, Czech Republic) was used. The limit of detection was calculated from the real lipocalin-2 values in wells and was 0.02 ng/mL. Blood-gas analysis was performed using the Siemens RAPIDPoint 500 Blood Gas System.
For NGAL level measurements, Human Lipocalin-2/NGAL ELISA kit Biovendor (Brno, Czech Republic) was used. The limit of detection was calculated from the real lipocalin-2 values in wells and was 0.02 ng/mL. Blood-gas analysis was performed using the Siemens RAPIDPoint 500 Blood Gas System.
7
Quantification of Inflammatory Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total white blood cells counts were quantified with an ADVIA-2120 system (Siemens, Germany). The plasma concentration of C-Reactive Protein (CRP) was determined by ultra-sensitive quantitative turbidimetric test (Bayer Diagnostics, Germany), and those of interleukin (IL)-6 (IL-6) and IL-8 by high sensitivity ELISA (Invitrogen, USA). Blood carboxyhemoglobin levels were determined with a Rapid Point 500 Blood-Gas-System (Siemens, Germany).
8
Hematological Analyses and Derived Ratios
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hematology analyses were performed using a Sysmex XN-550™ Hematology Analyzer (Sysmex America Inc., United States). Arterial blood gasometry was conducted on a RAPIDPoint® 500 blood gas system (Siemens Healthcare GmbH; Germany) under a controlled atmosphere. The calculation of the ratios was as follows: NLR, absolute neutrophil count divided by the absolute lymphocyte count; MLR, absolute monocyte count divided by absolute lymphocyte count; PLR, platelet count divided by absolute lymphocyte count; and MPR, mean platelet volume divided by platelet count.
9
Ovarian Follicle Viability Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The concentrations of glucose, lactate, potassium, sodium, calcium and chloride in the perfusion medium were measured by a standard blood-gas analysis according to the manufacturer's protocols (RapidPoint 500 Blood Gas System, Siemens Healthcare, Erlangen, Germany) to assess the ovarian Signed Rank test for related samples was used to compare the number of non-damaged and damaged follicles before perfusion, after cold ischaemia and after perfusion. Wilcoxon matched-pairs signed-rank test was used to compare the percentage of Ki-67 and cleaved caspase 3 positive follicle cells before and after perfusion. To assess any changes over time for hormonal-, metabolic-and electrolytic biomarker metabolic activity.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!