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C340 tmd a

Manufactured by Thorlabs
Sourced in United States

The C340 TMD-A is a compact and modular scientific instrument designed for precision optical measurements. It features a high-resolution tilt and tip platform that allows for accurate angular adjustments. The device is suitable for a variety of laboratory applications that require precise control and positioning of optical components.

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2 protocols using c340 tmd a

1

Single-Molecule Microscopy Setup for SMLM Imaging

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We adapted a standard upright widefield fluorescent microscope (Leica DM RXA) for SMLM acquisition. A 405 nm laser (LuxX + 405–120, Omicron) and a 488 nm laser (LuxX + 488–100, Omicron) were used for activation and excitation, respectively, which were coupled in using a polarising-maintaining optical fibre, all from Omicron-Laserage Laserprodukte GmbH, Germany. Standard Leica fluorescent cubes were used (FITC filter or N2.1 filter), with the excitation filters removed from the filter blocks. Fluorescence was collected using either a long-working distance lens (HCX PL Fluotar L 100x/0.75, Leica) in combination with a cryostage (CMS-196, Linkam Scientific, UK), or an oil-immersion lens for experiments at 294 K (PL Fluotar 100x/1.3, Leica). Fluorescent images were recorded by a sCMOS camera (pco.edge 4.2, DVision, Oostakker, Belgium).
Fluorescence emission spectra were recorded by a home-made set-up that was mounted on an extra camera port via a beam-splitter (100-50-0%, Leica). The emitted light was coupled in a multimode optical fibre (M15L01, Thorlabs Inc, USA) by an aspheric glass lens (C340 TMD-A, Thorlabs Inc, USA) and connected to a fibre-optic spectrometer (AvaSpec-2048L, Avantes, the Netherlands). Software written in-house was utilised to read-out the camera, and to synchronise the lasers and camera acquisition.
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2

Smartphone-based Fluorescence Imaging Platform

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The platform structure for assembling the smartphone and other optical/mechanical components was designed using AUTODESK® 123D® DESIGN and prototyped using a 3D printer (Form 2; FormLabs, Somerville, MA, USA). The following components were assembled on the platform: a power LED (365 nm at 3 W; Epileds, Taiwan), a battery-operated micro linear actuator (LA003; Seiko, Tokyo, Japan), a micrometer head (SHS2-13, Sigma), an aspherical lens (C340TMD-A; Thorlabs, Newton, NJ, USA), a long pass filter for emissions (λ = 410 nm, SC-41; Fujifilm, Tokyo, Japan), and a microcomputer system (Genuino 101; Arduino LLC, USA). The aspherical lens was set into the hole beneath which the smartphone camera was inserted. The long pass filter was placed between the aspherical lens and the FRAD (Fig. 1). The micro linear actuator enabled the lateral movement of the sample slide and enabled observation at a 1 cm distance. The micrometer head was used to move the sample slide in the z direction for focusing. The power LED and battery-operated linear actuator were operated via a Genuino 101 board, which communicated to the smartphone through a Bluetooth Low Energy (BLE) module. The size of the smartphone-based platform was approximately 23 × 10 × 7 cm (Fig. S1 †), and the cost was estimated to be <$360 (USD), excluding the smartphone.
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