Liquid nitrogen-frozen muscle samples were homogenized, and RNA was extracted using TRIsure (Bioline) and the Quick-RNA Miniprep kit (Zymo Research) per manufacturer instructions. Cell populations were sorted directly into lysis buffer, and RNA was isolated using the Quick-RNA Microprep kit (Zymo Research). Complementary DNA (cDNA) was synthesized from 150 ng (sorted cells) or 1000 ng (whole muscle) of DNase-treated RNA using the SensiFAST cDNA synthesis kit (Bioline). Gene expression was quantified using TaqMan expression assay probes (Life Technologies) and 2x SensiFAST probe No-ROX mix (Bioline). All gene expression was normalized to 18 s unless otherwise noted.
Taqman expression assay probes
Manufactured by Thermo Fisher Scientific
TaqMan expression assay probes are a type of molecular biology tool used for gene expression analysis. They are designed to detect and quantify specific RNA or DNA sequences in a sample. The probes utilize a fluorescent reporter dye and a quencher dye to provide a real-time, quantitative measurement of the target sequence.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using taqman expression assay probes
1
RNA Extraction and Gene Expression Analysis
2
RNA Extraction and Gene Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liquid nitrogen-frozen muscle samples were homogenized, and RNA was extracted using TRIsure (Bioline) and the Quick-RNA Miniprep kit (Zymo Research) per manufacturer instructions. Cell populations were sorted directly into lysis buffer, and RNA was isolated using the Quick-RNA Microprep kit (Zymo Research). Complementary DNA (cDNA) was synthesized from 150 ng (sorted cells) or 1000 ng (whole muscle) of DNase-treated RNA using the SensiFAST cDNA synthesis kit (Bioline). Gene expression was quantified using TaqMan expression assay probes (Life Technologies) and 2x SensiFAST probe No-ROX mix (Bioline). All gene expression was normalized to 18 s unless otherwise noted.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!