Wedelolactone was provided by Chengdu Pufei De Biotech Co., Ltd. (purity > 98.0%, Chengdu, China). Prednisone acetate was from Huazhong Pharmaceutical Co., Ltd. (Xiangyang, China). Doxorubicin hydrochloride for injection was obtained from KeyGen. Co. Ltd. (Nanjing, China). Lecithin (average molecular weight: 760 g/mol) was purchased from Shanghai Advanced Vehicle Technology Pharmaceutical Ltd (Shanghai, China). Solutol® HS15 (molecular weight: 963 g/mol) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetic acid, acetonitrile, and methanol were Chromatographic Grade and were obtained from Tedia Company Inc. (Fairfield, USA). A 2′,7′-dichlorofluorescein diacetate (DCFDA)-cellular ROS detection assay kit was obtained from Abcam (Cambridge, UK). The other reagents are analytical.
Solutol hs15
Manufactured by Merck Group
Sourced in United States, Germany
Solutol HS15 is a non-ionic solubilizer and emulsifier used in the pharmaceutical and chemical industries. It is a polyethylene glycol 15 hydroxystearate surfactant. Solutol HS15 is used to enhance the solubility and stability of various active ingredients and formulations.
Automatically generated - may contain errors
Lab products found in correlation
Tween 80, by Merck Group (4 mentions)
Capryol 90, by Gattefosse (3 mentions)
Labrafil m 1944 cs, by Gattefosse (3 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (3 mentions)
Plurol oleique, by Gattefosse (2 mentions)
Peg400, by Merck Group (2 mentions)
Transcutol p, by Gattefosse (2 mentions)
Labrasol, by Gattefosse (2 mentions)
Labrafac, by Gattefosse (2 mentions)
Labrafil m 2125 cs, by Gattefosse (2 mentions)
Sp600125, by Cayman Chemical (2 mentions)
Transcutol hp, by Gattefosse (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
N methyl pyrrolidone (nmp), by Merck Group (2 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (2 mentions)
2 7 dichlorofluorescein diacetate dcfda cellular ros detection assay kit, by Abcam (1 mentions)
Methanol, by Tedia (1 mentions)
Acetic acid, by Tedia (1 mentions)
Acetonitrile, by Tedia (1 mentions)
33 protocols using solutol hs15
1
Wedelolactone-Loaded Nanoformulation for Cancer Therapy
2
Sorafenib and Ganoderic Acid Formulation
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Sorafenib (free base) was purchased from Active Biochem, Billion Centre, Hong Kong. Ganoderic Acid (GA) was received from Sigma Aldrich, USA. Capmul MCM C10 and Capmul PG8 were purchased from M/s Gattefosse, Cedex, France. Precirol® ATO5 and Solutol HS15 were purchased from Sigma-Aldrich Inc., Alabama, USA. Solvents with HPLC grades were purchased from local vendors. Span Diagnostics, Surat, India, supplied the kits for detection of the serum sickness Alkaline Phosphatase Test (APT), albumin, Aspartate Transaminase (AST) Test, Alanine transferase (ALT) and total protein.
3
Xenograft Tumor Growth Inhibition
4
Cytotoxicity Evaluation of Lipid-Based Formulations
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NAR (purity > 95%), Solutol HS15, Tween 20, Tween 80, Chremophor EL, PEG 400, propylene glycol, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and chitosan (low-molecular weight) were obtained from Sigma Aldrich (St. Louis, MO, USA). Plurol Oleique, Labrafac, Transcutol P, Labrafil M1944 CS, Labrafil M2125 CS, Labrasol, Lauroglycol 90 and Capryol 90 were gifted by Gattefosse, France and Sefsol 218 was gifted by Nikko Chemicals Co. Ltd., Tokyo, Japan. Invitrogen (Carlsbad, CA, USA) supplied Roswell Park Memorial Institute (RPMI)-1640, fetal bovine serum (FBS)-heat-inactivated and antibiotics including other materials for cell culture. Sunflower oil, oleic acid, castor oil and peanut oil were obtained from local suppliers. Deionized water and analytical grade chemicals were used in each step of the experiments, where the chemicals were used without further purification.
5
Xenograft Tumor Growth Inhibition
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Cell line xenograft experiments were performed in female C.B-17 SCID mice age 8 weeks by injecting 5 X 106 PC9-RR, PC9-OR tumor cells within 50% matrigel. Tumors were allowed to grow until they reached a minimum volume of 150 mm3 and mice were randomized to receive treatment by oral gavage seven days per week for 71 days. Rociletinib was formulated using 5% DMSO, 15% Solutol HS15 (Sigma Inc) in 80% water and osimertinib was formulated in 1% DMSO, 30% PEG300 and 69% water. MLN8237 was formulated using 10% (2-Hydroxypropyl)-β-cyclodextrin in water. Tumor growth was assessed twice weekly by caliper measurements. A minimum of 10 tumors per treatment group were assessed for the duration of the study. For PDX xenografts, patient derived tumor cells were engrafted subcutaneously into the flank C.B-17 SCID mice. Tumors were allowed to grow until they reached a minimum volume of 200 mm3 and then randomly placed into control or treatment groups. Animals were treated daily for 30 days via oral gavage and tumor volume was calculated daily using caliper measurement. Percentage change for tumor growth was based on volumes calculated from size on day 1 at the beginning of treatment. All animal studies were conducted in accordance with the UCSF Institutional Animal Care and Use Committee (IACUC) and all relevant ethical regulations were followed.
6
Quantitative Analysis of Ginsenoside Compounds
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DA-9805 (Lot No. PD15702; containing 0.456% SSa, 5.919% PA, and 0.576% IMP, as determined by HPLC-UV) was obtained from Dong-A ST (Yongin, Korea). SSa (98.32% purity) and IMP (99.17% purity) were products from Letopharm, Ltd. (Shanghai, China), and PA (99.8% purity) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Internal standard (IS) for SSa, PA, and IMP (dexamethasone, bumetanide, indomethacin, respectively), hydroxypropyl-methylcellulose (HPMC), dimethylsulfoxide (DMSO), Tween® 80, and Solutol® HS 15 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Other chemicals were of reagent or HPLC grade.
7
Combination Therapy of Antimalarial and Antifungal
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Griseofulvin (Sigma-Aldrich) was prepared by dissolving 1 or 2 mg in 40 μl DMSO and then making up the volume to 200 μl with 10% solutol HS 15 (Sigma-Aldrich) in saline. The mixture was vortexed thoroughly for 10 min to form an emulsion and injected intraperitoneally into the mice. All single dose injections were carried out at 06:00 h and double dose injections were carried out at 06:00 h and 18:00 h for the respective days. Control mice were injected with the solvent. α,β-arteether (E MAL (75 mg/ml); Themis Medicare Ltd) was administered intramuscularly. For experiments involving the combination of α,β-arteether and Griseofulvin, α,β-arteether was administered intramuscularly at 06:00 h on day 6, followed by two doses of Griseofulvin at 24 h interval (at 09:00 h on day 6 and 7).
8
Tumor Growth Inhibition by Radiation and CCR2/CCR5 Antagonist
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A general tumor protocol was established where 1 x 105 tumor cells were injected intramuscularly in the left legs of female C57BL/6J or BALB/cJ mice. Mice were treated locally with radiotherapy (RT) 7 days after tumor cell injection using a 3200 Curie-sealed 137Cesium source that operates at roughly 1.90 Gy/min. Jigs were constructed and designed to specifically treat the tumor-bearing leg with 15 Gy radiation [2 (link)]. This source and the collimators used are calibrated periodically to ensure equal distribution of radiation. Standard calipers were used to measure tumor growth as described previously [53 (link)]. Tumor-bearing mice were administered 15 mg/kg of a CCR2/CCR5 antagonist (named CVC, Tobira Therapeutics, CA) [54 (link), 55 (link)] or vehicle control (40% Hydroxypropyl-beta-cyclodextrin [Acros Organics] & solutol HS15 [Sigma] in sterile water) subcutaneously (s.c.) 1X/day starting 2 days before RT for the indicated amount of time. CD8+ T cells were depleted by treating mice with 200 ug of anti-CD8 (clone 53-6.7) i.p. every 3 days beginning 4 days post-tumor inoculation. Rat IgG was used as a control in anti-CD8 experiments and did not affect tumor growth when compared to mice that did not receive rat IgG (data not shown).
9
PARP Inhibitor Effects on Lung and Pancreatic Cancer Cells
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All cell lines were used within 6 months of purchase. H460 (wild type p53), H1299 (p53 null), Miapaca2 (mutations in k-ras and p53) were obtained from the American Type Culture Collection (ATCC). PDA cell line (mutations in k-ras and p53) was a kind gift from Dr. Edgar G. Engleman of Stanford University. H1299 and H460 lung cancer cells were maintained in RPMI1640 with 10% fetal bovine serum (FBS). PDA cells were grown in DMEM with 10% FBS and 1% non-essential amino acids. Miapaca2 cells were maintained in DMEM with 10% FBS and 2.5% horse serum. All cell line media also contained 1% v/v penicillin-streptomycin (100 IU/mL, 100 mg/mL; Thermo Scientific Carlsbad, CA.). All cells were propagated at 37°C in a humidified atmosphere maintained at 5% CO2. PARP inhibitor, LT626 was a gift from BioMarin Pharmaceutical Inc. (San Rafael, CA). Synthesis of LT626 has been described previously by McPherson et al [26 (link)]. Solutol HS-15, Dimethyl sulfoxide (DMSO), Methylene blue and MTT was purchased from Sigma Chemicals (St. Louis, MO). N,N-Dimethylacetamide (DMAC) was purchased from EMD Millipore (Billerica, MA).
10
PLGA-based Nanoparticle Formulation
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Poly-dl -lactide-co-glycolide (PLGA) (Purasorb® PDLG 7507; with a molar ratio of 75/25 and an inherent viscosity midpoint of 0.7 dl/g) was gently provided from Corbion Purac Biomaterials (Amsterdam, The Netherlands). The lecithin, Soybean phosphatidylcholine (SPC, Lipoid® S100) was kindly supplied by Lipoid GmbH (Ludwigshafen am Rhein, Germany). TPGS was generously delivered by Isochem (Vert-le-Petit, France). Tween® 80 and Solutol® HS 15 were obtained from Sigma-Aldrich, Chemical Co. (Steinheim, Germany). Methanol (HPLC grade) was purchased from Fluka (Buchs, Switzerland). Potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium chloride, potassium chloride (KCl), acetone, dimethyl sulfoxide (DMSO) and ortho-phosphoric acid were obtained from Adwia, El-Nasr Pharmaceutical Co. (Egypt). Spectra/Por® dialysis membrane, 12,000–14,000 MWCO was purchased from Spectrum Medical Industries (Houston, TX, USA). Nanosep® centrifuge tubes, fitted with an ultra-filter of 100 kDa MWCO, were provided from Pall Life Sciences (East Hills, NY, USA). Deionized water provided from Milli-Q Gradient A10 System was employed all over the research study. All other chemicals and reagents were of analytical grade.
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