Anti rab5a

The following primary antibodies were used: anti-GAPDH (Sigma-Aldrich, G9545; 1:5000) anti-TOMM20 (Santa Cruz Biotechnology, SC-11415; 1:500), anti-FIS1 (Proteintech, 10956–1-1ap; 1:100), anti-ATP5F1A/ATP5A (Abcam, Ab14748; 1:200), anti-PDHA1 (Abcam, ab110330; 1:200), anti-ATG7 (Cell Signaling Technology, 8558; 1:1000), anti-RAB9A (Cell Signaling Technology, 5118; 1:1000), anti-ULK1 (Cell Signaling Technology, 8054; 1:1000), anti-RAB5A (Cell Signaling Technology, C8B1; 1:1000), anti-RAB7 (ERP7589; Abcam, ab137029; 1:1000), anti-LC3B (Sigma-Aldrich, L7543;1:200). Alexa Fluor 647‐conjugated goat anti-rabbit and anti-mouse IgG (Invitrogen, A21244 and A32728; 1:500) were used as secondary antibodies.

Protein samples were resolved on 10%–12% SDS‐PAGE and transferred to nitrocellulose membranes. MEF protein samples were resolved on Criterion TGX precast gels (4%–20%). Antigen–primary antibody complexes were incubated with horseradish‐peroxidase (HRP)‐conjugated secondary antibodies and visualised by Western blotting luminol reagent (Thermo USA). Anti‐APJ (Millipore‐Sigma #ABD43), anti‐apelin (LSbio‐Rabbit mAb #LS‐C149244‐200), anti‐Rab5a (Cell Signaling‐Mouse mAb #46449), anti‐caveolin‐1 (Cell Signaling‐Rabbit mAb #3267), anti‐clathrin‐heavy chain (Cell Signaling‐Rabbit mAb #4796), anti‐EEA‐1 (Cell Signaling‐Rabbit mAb #3288), anti‐Rab7 (Cell Signaling‐Rabbit mAb #9367), anti‐Rab‐11 (Cell Signaling‐Rabbit mAb #5589), anti‐pERK1/2 (Cell Signaling‐Rabbit mAb #9101), anti‐tERK1/2 (Cell Signaling, mAb #9102), anti‐α2a adrenergic receptor (Alomone‐Rabbit mAb#AAR‐020), anti‐β3 adrenergic receptor (Abcam‐Rabbit mAb#ab94506), anti‐caspase‐3 (Cell Signaling, mAb #9662), anti‐ATPase (Na‐K) α1 (DSHB‐A6F‐C) and anti‐GAPDH (R&D Systems‐Rabbit mAb #2275‐PC‐020) were used at a working dilution of 1:1000. Images were captured and quantified using Image Lab (BioRad, USA) software, and intensity values were normalised to Gapdh. For the MEF, the protein band in the stain‐free gel image was used for the normalisation. The uncropped blots are included in the Figures S12 and S13.