Human il 1β elisa kit

Manufactured by Neobioscience

Sourced in China

The Human IL-1β ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human interleukin-1 beta (IL-1β) in biological samples such as cell culture supernatants, serum, and plasma.

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IL-1β (21 citations)

6 protocols using human il 1β elisa kit

Quantification of Cytokine Levels

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The levels of IL-1β and IL-18 after the indicated drug treatment were measured using the Human IL-1β ELISA Kit (NEOBIOSCIENCE, Shenzhen, China) and Human IL-18 ELISA Kit (NEOBIOSCIENCE) according to the recommended protocol. Moreover, CD8+ T cell culture supernatants were collected and the levels of IL-2 and IFN-γ were quantified by Human IL-2 ELISA Kit (NEOBIOSCIENCE) and Human IFN-γ ELISA Kit (NEOBIOSCIENCE) following the manufacturers’ instructions. Each sample was tested in triplicate.

Ren J., Tao Y., Peng M., Xiao Q., Jing Y., Huang J., Yang J., Lin C., Sun M., Lei L., Yang Z., Yang Z, & Zhang L. (2022). Targeted activation of GPER enhances the efficacy of venetoclax by boosting leukemic pyroptosis and CD8+ T cell immune function in acute myeloid leukemia. Cell Death & Disease, 13(10), 915.

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Cell Death and IL-1β Quantification

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To determine cell death, the CytoTox 96^® Non-Radioactive Cytotoxicity Assay kit (G1780, Promega) was used to quantify lactate dehydrogenase (LDH) release from dTHP-1 variants. The release of IL-1β was quantified with a Human IL-1β ELISA kit (EHC002B, NeoBioscience) following the manufacturer’s protocol.

Zhao Y, & Sun L. (2022). Bacillus cereus cytotoxin K triggers gasdermin D-dependent pyroptosis. Cell Death Discovery, 8, 305.

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Cytokine Levels Under SFP and DSFP-45

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The effects of SFP and DSFP-45 on the levels of pro-inflammatory cytokines, including IL-1β, IL-6, and TNF-α, were studied by ELISA. The level of IL-1β was measured using the Human IL-1β ELISA Kit (EHC002b.48, NeoBioscience Technology Co., Ltd., Shenzhen, China) according to the manufacturer’s protocol. The content of IL-6 was determined using the Human IL-6 ELISA Kit (70-EK106/2-96, Multi Sciences Biotech Co., Ltd., Hangzhou, China). The amount of TNF-α was detected by the Human TNF-α ELISA Kit (70-EK182-96, Multi Sciences Biotech Co., Ltd., Hangzhou, China).

Yao W., Yong J., Lv B., Guo S., You L., Cheung P.C, & Kulikouskaya V.I. (2023). Enhanced In Vitro Anti-Photoaging Effect of Degraded Seaweed Polysaccharides by UV/H2O2 Treatment. Marine Drugs, 21(8), 430.

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Cytokine Secretion Profiling in Cell Lines

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SCC25 and FaDu cells were seeded into six-well plate and treated for 24 and 48 hours, respectively. The supernatants were collected and centrifuged under 2000 × g for 10 minutes to eliminate cell debris. Human IL-1β ELISA kit, Human IL6 ELISA kit, Human IL8 ELISA kit (NeoBioscience, Guangdong, China), and Human HMGB1 ELISA kit (Telenbiotech, Guangzhou, China) were used to detect the concentration of indicated molecules following the manufacture’s instruction.

Li J., Huang S., Zeng L., Li K., Yang L., Gao S., Guan C., Zhang S., Lao X., Liao G, & Liang Y. (2020). Necroptosis in head and neck squamous cell carcinoma: characterization of clinicopathological relevance and in vitro cell model. Cell Death & Disease, 11(5), 391.

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BEAS-2B Cytokine Secretion Assay

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The supernatant media derived from the untreated control, BEAS-2B treated with TNF-α and IFN-β for 24 h and 48 h, were collected, and the concentration of IL-1β in which was measured using a human IL-1β ELISA kit (Neobioscience, Shenzhen, China), according to the instructions of the manufacturer.

Sun R., Jiang K., Zeng C., Zhu R., Chu H., Liu H, & Du J. (2022). Synergism of TNF-α and IFN-β triggers human airway epithelial cells death by apoptosis and pyroptosis. Molecular Immunology, 153, 160-169.

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Intracellular cAMP and IL-1β Quantification

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The detection of intracellular cAMP was performed using cAMP-Glo Assay (Promega, USA). The levels of IL-1β in culture supernatants were measured using human IL-1β ELISA kit (NeoBioScience, China). All procedure were conducted strictly according to the manufacturer’s instructions and analyzed on a grating microplate reader (Corona SH-1000Lab, Hitachi, Ltd, Japan).

Li H., Jiang W., Ye S., Zhou M., Liu C., Yang X., Hao K, & Hu Q. (2020). P2Y14 receptor has a critical role in acute gouty arthritis by regulating pyroptosis of macrophages. Cell Death & Disease, 11(5), 394.

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