Hydrochlorothiazide hctz

Angiotensin II, hydrochlorothiazide (HCTZ), CGP42112A, losartan and PD123319 were purchased from Sigma (St Louis, MO). The NCC antibody was a gift from Robert Hoover at Emory University, whereas antibody of phosphorylated NCC at threonine 53 (Thr⁵³) was kindly provided by David H. Ellison at Oregon Health & Science University. The data are shown as mean ± SEM. We used the paired t test or one-way ANOVA test to determine the statistical significance.

Na36Cl and H36Cl were from ICN (Irvine, CA) and Na₂³⁵SO₄ from ARC (Saint Louis, MO). ¹⁴C-oxalate originally from NEN-DuPont (Boston, MA) was a gift from C. Scheid and T. Honeyman (Univ. Mass. Med. Ctr.). Restriction enzymes and T4 DNA ligase were from New England Biolabs (Beverly, MA). EXPAND High-fidelity PCR System was from Roche Diagnostics (Indianapolis, IN). QuikChange II XL site-directed mutagenesis kit was from Agilent Technologies (Lexington, MA). MEGAscript^® T7 Transcription Kit was from Thermo Fisher Scientific (Waltham, MA). 4,4'-Diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) was from Calbiochem (La Jolla, CA). Phorbol-12-myristate-13-acetate (PMA) was from LC Laboratories (Woburn, MA). Niflumic acid (NFA), glyoxylate, dibutyryl cyclic AMP sodium salt (dB-cAMP), 3-Isobutyl-1-methylanxthine (IBMX), tenidap and 1,2-Bis(2-aminophenoxy)ethane -N,N,N’,N’-tetraacetic acid tetrakis (acetoxymethyl ester) (BAPTA-AM) were from Santa Cruz Biotechnology (Dallas, TX). Hydrochlorothiazide (HCTZ) and R-(+)-[(2-n-Butyl-6,7-dichloro-2-cyclopentyl-2,3-dihydro-1-oxo-1H-inden-5-yl)oxy]-acetic acid (DIOA) were from Sigma (St. Louis, MO). DMSO Stock solutions of these drugs were stored at −20 °C. All other chemical reagents were from Sigma or Fluka (Milwaukee, WI) and were of reagent grade.