Protein a dynabead magnetic beads

Manufactured by Thermo Fisher Scientific

Protein A Dynabead magnetic beads are spherical superparamagnetic beads coated with recombinant Protein A, a bacterial protein that binds to the Fc region of immunoglobulins. These beads can be used to capture and isolate antibodies from complex biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Stat6 antibody, by Cell Signaling Technology (1 mentions) Srebp1 antibody, by Santa Cruz Biotechnology (1 mentions) Epitect chip pcr assay, by Qiagen (1 mentions)

Close spelling variants of this product

Dynabeads (2112 citations) Dynabeads Protein A (819 citations) Magnetic beads (264 citations) Magnetic Dynabeads (60 citations) Dynabeads magnetic beads (38 citations) Dynabeads Protein A beads (29 citations) Dynabead Protein A (16 citations) Dynabeads Protein A magnetic beads (12 citations) Protein A Magnetic Dynabeads (8 citations) Dynabead magnetic beads (3 citations)

2 protocols using protein a dynabead magnetic beads

Chromatin Immunoprecipitation (ChIP) Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

ChIP was performed as described previously^{62 (link)} using Stat6 antibody (Cell signaling Technology, 5397). Briefly, 5 μg rabbit IgG (Santa Cruz) or Stat6 antibody was incubated with Protein A Dynabead magnetic beads (Invitrogen) for 4 h, followed by extensive washing to remove unbound antibody. Antibody beads were then added to the chromatin and incubated overnight.

Dey P., Li J., Zhang J., Chaurasiya S., Strom A., Wang H., Liao W.T., Cavallaro F., Denz P., Bernard V., Yen E.Y., Genovese G., Gulhati P., Liu J., Chakravarti D., Deng P., Zhang T., Carbone F., Chang Q., Ying H., Shang X., Spring D.J., Ghosh B., Putluri N., Maitra A., Wang Y.A, & DePinho R.A. (2020). Oncogenic Kras driven metabolic reprogramming in pancreas cancer cells utilizes cytokines from the tumor microenvironment. Cancer discovery, 10(4), 608-625.

+ Open protocol

+ Expand

ChIP Assay for SREBP1 Binding

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

ChIP was performed as described previously^{31 (link)}using SREBP1 antibody (Santa Cruz, sc-367x). Briefly, 5 μg rabbit IgG (Santa Cruz) or SREBP1 antibody was incubated with Protein A Dynabead magnetic beads (Invitrogen) for 4 h, followed by extensive washing to remove unbound antibody. Antibody beads were then added to the chromatin and incubated overnight. The ChIP primers were purchased from Qiagen (EpiTect ChIP PCR assay) and used for qPCR analysis: BCAT2 (GPH1020848(+)03A).

Dey P., Baddour J., Muller F., Wu C.C., Wang H., Liao W.T., Lan Z., Chen A., Gutschner T., Kang Y., Fleming J., Satani N., Zhao D., Achreja A., Yang L., Lee J., Chang E., Genovese G., Viale A., Ying H., Draetta G., Maitra A., Wang Y.A., Nagrath D, & DePinho R.A. (2017). Genomic deletion of malic enzyme 2 confers collateral lethality in pancreatic cancer. Nature, 542(7639), 119-123.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!